Expression of hypoxia-inducible factor-1 in the cochlea of newborn rats

Gross, Johann; Rheinländer, Cornelia; Fuchs, Julia; Mazurek, Birgit; Machulik, Astrid; Андреева, Н. И.; Kietzmann, Thomas

doi:10.1016/s0378-5955(03)00222-3

Cited by 24 publications

(7 citation statements)

References 49 publications

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“…In fact, the most hypoxia sensitive cells, such as the ganglion neurons and the Schwann cells 36 are effectively localized in the region of the SG. These results are in line with findings of Stroka et al 37 and Gross et al 38 who showed that functionally different cochlea regions explanted in vitro have a specific HIF1a expression. In our in vivo study, we demonstrated clearly that HIF-1a protein, effectively expressed in specific areas, is the cogent demonstration of the presence of a hypoxic environment in the cochlea, as previously reported.…”

Section: Discussionsupporting

confidence: 92%

Hypoxia-inducible factor 1-alpha target protein up-regulation in Hypoxic cochlear neurons is associate with aged-related hearing loss in C57BL/6 mice

Donadieu

Riva

2012

Ageing Res

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Molecular mechanisms underlining hypoxia-induced aged-hearing loss were studied. 3-months C57BL/6 mice were subjected to four weeks of hypoxia (10% 0 2 ), whereas, controls were kept under normoxic condition for up to six months. Auditory function was explored by CAP and Preyer's reflex measurements and correlated with histological analysis of the cochlea. The presence of oxidative damage, HIF-1 responsive target genes regulation involved in cell death, inflammation and neovascularization were assessed by immunofluorescence analysis. Hypoxia was associated to severe hearing loss at 4-8 and 16 KHz and degeneration of the cochlea, with significant cell loss (30%) in the spiral ganglion, the lateral wall, and the hair cells with a basal-apical alteration gradient. This was correlated with ROS formation and HIF-1a overexpression. Cochlear degeneration was due to apoptosis via activated caspase-3, P53, Bax and Bcl-2 protein differential expression in spiral ganglion, modiolus and spiral ligament. On the other hand, Hsp70, NF-kB transcription factor pathway and inflammatory mediators (caspase-1 and TNF-a) were induced in the stria vascularis. Furthermore, a phenomenon of neovascularization was observed with significant thickening of stria vascularis and increased expression of VEGF. In total, we demonstrated that the tandem-HIF-ROS is responsible for the caspase-3 and Bax-mediated apoptosis via P53 protein accumulation in the cochlear neurons, while inflammatory response mediated by Hsp70 stress protein and NF-kB transcription factor generating a neovascularization phenomenon occurred in stria vascularis.

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Section: Discussionsupporting

confidence: 92%

Hypoxia-inducible factor 1-alpha target protein up-regulation in Hypoxic cochlear neurons is associate with aged-related hearing loss in C57BL/6 mice

Donadieu

Riva

2012

Ageing Res

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“…Our Wndings provide new evidence that auditory cells are related to HIF-1 expression under hypoxic conditions. Gross et al (2003) reported that HIF-1 is expressed in the cochlea of newborn rats by hypoxia. NF-kB, a ubiquitous transcription factor that plays a major role in the regulation of stress-related genes, is activated during environmental hypoxia in the dorsocaudal brainstem of adult rats (Simakajornboon et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Hypoxia-induced IL-6 production is associated with activation of MAP kinase, HIF-1, and NF-κB on HEI-OC1 cells

Jeong¹,

Hong²,

Park³

et al. 2005

Hearing Research

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“…Immunohistochemical evidence in rodents suggests that neurotrophin signaling increases first in the cochlea, and subsequently, in brainstem and cortical auditory regions during a period that begins one week before hearing onset and ramps up during the first month of life [ 24 – 27 ]. In contrast, Hif1a mRNA expression has been identified in cochlear explants and dissociated cochlear preparations of one-week old rats, but it has not been investigated in the central auditory system during postnatal development [ 28 ]. The limited availability of gene expression screens from multiple auditory brain regions represents a major challenge to understand the relationship between different signaling pathways and auditory system development.…”

Section: Introductionmentioning

confidence: 99%

Defining the relationship between maternal care behavior and sensory development in Wistar rats: Auditory periphery development, eye opening and brain gene expression

et al. 2020

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Defining the relationship between maternal care, sensory development and brain gene expression in neonates is important to understand the impact of environmental challenges during sensitive periods in early life. In this study, we used a selection approach to test the hypothesis that variation in maternal licking and grooming (LG) during the first week of life influences sensory development in Wistar rat pups. We tracked the onset of the auditory brainstem response (ABR), the timing of eye opening (EO), middle ear development with micro-CT X-ray tomography, and used qRT-PCR to monitor changes in gene expression of the hypoxia-sensitive pathway and neurotrophin signaling in pups reared by low-LG or high-LG dams. The results show the first evidence that the transcription of genes involved in the hypoxia-sensitive pathway and neurotrophin signaling is regulated during separate sensitive periods that occur before and after hearing onset, respectively. Although the timing of ABR onset, EO, and the relative mRNA levels of genes involved in the hypoxia-sensitive pathway did not differ between pups from different LG groups, we found statistically significant increases in the relative mRNA levels of four genes involved in neurotrophin signaling in auditory brain regions from pups of different LG backgrounds. These results suggest that sensitivity to hypoxic challenge might be widespread in the auditory system of neonate rats before hearing onset, and that maternal LG may affect the transcription of genes involved in experience-dependent neuroplasticity.

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Expression of hypoxia-inducible factor-1 in the cochlea of newborn rats

Cited by 24 publications

References 49 publications

Hypoxia-inducible factor 1-alpha target protein up-regulation in Hypoxic cochlear neurons is associate with aged-related hearing loss in C57BL/6 mice

Hypoxia-inducible factor 1-alpha target protein up-regulation in Hypoxic cochlear neurons is associate with aged-related hearing loss in C57BL/6 mice

Hypoxia-induced IL-6 production is associated with activation of MAP kinase, HIF-1, and NF-κB on HEI-OC1 cells

Defining the relationship between maternal care behavior and sensory development in Wistar rats: Auditory periphery development, eye opening and brain gene expression

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