Expression of hyaluronic acid in N-nitrosodimethylamine induced hepatic fibrosis in rats

George, Joseph; Tsutsumi, Mikihiro; Takase, Shujiro

doi:10.1016/s1357-2725(03)00253-x

Cited by 46 publications

(41 citation statements)

References 38 publications

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“…The increased acid mucopolysaccharide in the areas might be the result of inflammatory process. It has been suggested that glycosaminoglycan, hyaluronic acid, is involved in the pathogenesis of liver diseases and its increase in the liver and serum has been used as a reliable marker of hepatic fibrosis and fibrogenesis (Frebourg et al, 1986;Murawaki et al, 1996;Peres et al, 1996;George et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Pathological changes and local defense reaction occurring in spontaneous hepatic coccidiosis in rabbits (Oryctolagus cuniculus).

Darzi¹,

Mir²,

Kamil³

et al. 2010

World rabbit sci.

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Hepatic coccidiosis occurred in 56 rabbits belonging to different ages, sexes and breeds during the years from 2002 to 2005. Clinically, the severely affected rabbits showed decreased growth rate, anorexia, debilitation, listlessness, diarrhea, icterus and rough hair coat. Postmortem examinations revealed increased dirty dull straw colored peritoneal fluid. Hepatomegaly, with presence of discrete yellowish-white nodules of 1mm to 1 cm size on the surface and throughout the parenchyma, was seen. Smears from peritoneal fluid showed rafts of mesothelial cells. Impression smears from the liver nodules revealed presence of numerous developmental stages of Eimeria stiedae corresponding with the stage of the liver lesion. These were intermixed with sheats of hepatobiliary cells and inflammatory cells and the changes were reflective of the histological changes like biliary hyperplasia, cholangitis and vacuolar changes occurring in the liver. There was increase in the thickness of basement membrane with the advanced stage of the lesion. The basement membrane was up to 1-2 µm in thickness forming loop like structures over which resided hyperplastic biliary cells containing different stages of the parasite. Some thickened basement membranes were devoid of overlying cells which had desquamated and were present in the ductal lumens suggesting that the basement might have the local defensive role in warding off the coccidial infection. The basement membrane was positive for neutral mucopolysaccharides while as the inflammatory zones and biliary epithelia along with the gametogonic stages of the coccidium reacted positively to the increased acid mucopolysaccharides qualitatively. The results showed that the occurrence of hepatic coccidiosis in female rabbits coincided with the breeding and kindling periods. Direct wet mount smears from bile aid in diagnosis of the disease. Impression smears from the liver nodules might represent the histological changes in the liver. The basement membrane thickening might have a defensive role in warding off the coccidial infection.

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Section: Discussionmentioning

confidence: 99%

Pathological changes and local defense reaction occurring in spontaneous hepatic coccidiosis in rabbits (Oryctolagus cuniculus).

Darzi¹,

Mir²,

Kamil³

et al. 2010

World rabbit sci.

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“…This linear polysaccharide is a constituent of the extracellular matrix of peripheral tissues (7). Its concentration in the serum increases as liver fibrosis progresses because of an increase in its synthesis by activated hepatic stellate cells and in later fibrosis stages also because of reduced clearance by the liver sinusoidal endothelial cells (8,9). In patients with clinically significant fibrosis, ␣ 2 -macroglobulin concentrations also increase (10).…”

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confidence: 99%

GlycoFibroTest Is a Highly Performant Liver Fibrosis Biomarker Derived from DNA Sequencer-based Serum Protein Glycomics

Vanderschaeghe

Laroy

Sablon

et al. 2009

Molecular & Cellular Proteomics

111

119

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Liver fibrosis is currently assessed by liver biopsy, a costly and rather cumbersome procedure that is unsuitable for frequent patient monitoring, which drives research into biomarkers for this purpose. To investigate whether the serum N-glycome contains information suitable for this goal, we developed a 96-well plate-based serum N-glycomics sample preparation protocol that only involves fluid transfer steps and incubations in a PCR thermocycler yielding 8-aminopyrene-1,3,6-trisulfonic acid-labeled N-glycans. These N-glycans are then ready for analysis on the capillary electrophoresis-based DNA sequencers that are the current standard in clinical genetics laboratories worldwide. Subsequently we performed a multicenter, blinded study of 376 consecutive chronic hepatitis C virus patients for which liver biopsies and extensive serum biochemistry data were available. Among patients, the METAVIR fibrosis stage distribution was as follows: 10.6% F0, 44.4% F1, 20.5% F2, 18.4% F3, and 6.1% F4. We found that the ratio of two N-glycans, here called GlycoFibroTest, correlates with the histological fibrosis stage equally well as FibroTest ( ‫؍‬ 0.4 -0.5 in F1-

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“…The transformation of quiescent HSC into myofibroblast-like cells with the expression of cytoskeletal protein a-smooth muscle actin (a-SMA) initiates the chronic process of hepatic fibrosis, finally leading to the fatal stage of liver cirrhosis. 11 A few papers have recently reported that H. pylori caused liver damages as an independent etiological factor, and that the gene belonging to it was demonstrated by PCR in mice. 12 After being infected with H. pylori for 2 years, the mice developed primary HCC, and bacteria were shown by immunohistopathological staining.…”

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confidence: 99%

Helicobacter pylori promotes hepatic fibrosis in the animal model

Goo

Lee

et al. 2009

Laboratory Investigation

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Helicobacter pylori infection has been reported to be very common in patients with chronic liver diseases, including cirrhosis. To elucidate the pathological effect of H. pylori infection on the progression of hepatic fibrosis, C57BL/6 mice and Sprague-Dawley rats were orally inoculated with H. pylori, and hepatic fibrosis was induced with carbon tetrachloride (CCl 4 ) administration. We observed the histopathological changes and the presence of H. pylori genes by PCR in the liver. Significant increase in the fibrotic score as well as in serum alanine aminotransferase and aspartate aminotransferase levels was shown in the CCl 4 þ H. pylori group compared with that in the CCl 4 -treated group. Compared with the CCl 4 -treated group, a-smooth muscle actin and transforming growth factor-b1 were enhanced; however, senescence marker protein-30, a multifunctional protein protecting hepatocytes against oxidative stress and apoptosis, was suppressed in the CCl 4 þ H. pylori group. The 16S rRNA (400 bp) was demonstrated by PCR for H. pylori genes from genomic DNA extracted from the liver, and H. pylori-infected mice showed 93.8% (15 of 16) seropositivity by contrast with seronegativity in all H. pylori-noninfected mice. In addition, immunohistochemical study against H. pylori showed positive antigen fragments in the liver of the infected groups. Consequently, our data suggest that H. pylori infection could be an important contributing infectious factor to the development of liver cirrhosis.

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Expression of hyaluronic acid in N-nitrosodimethylamine induced hepatic fibrosis in rats

Cited by 46 publications

References 38 publications

Pathological changes and local defense reaction occurring in spontaneous hepatic coccidiosis in rabbits (Oryctolagus cuniculus).

Pathological changes and local defense reaction occurring in spontaneous hepatic coccidiosis in rabbits (Oryctolagus cuniculus).

GlycoFibroTest Is a Highly Performant Liver Fibrosis Biomarker Derived from DNA Sequencer-based Serum Protein Glycomics

Helicobacter pylori promotes hepatic fibrosis in the animal model

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