Expression of human β‐defensin‐3 in gingival epithelia

Abstract

This study shows that hBD-3 is frequently expressed in gingival epithelia. The appropriate expression of hBD-3 peptide may contribute to the maintenance of periodontal homeostasis, possibly through its antimicrobial effect and promotion of adaptive immune responses.

Periodontal Disease and Gingival Innate Immunity – Who Has the Upper Hand?

“…In addition, compared to the samples from healthy subjects, the level of beta-defensin mRNA expression was lower and less frequently found in samples from periodontitis patients (Dunsche, Acil et al 2002;Bissell, Joly et al 2004). Similar results have also been reported testing mRNA level by in situ hybridization and protein level using immunohistochemistry (Lu, Jin et al 2004;Lu, Samaranayake et al 2005;Hosokawa, Hosokawa et al 2006). These studies suggest a decrease in the expression of hBD-2 and hBD-3 in both patient groups of gingivitis and periodontitis.…”

“…The protein expression of hBD-1 and hBD-2 was mostly found in the granular and spinous cell layer in healthy and diseased gingival tissue samples (Lu, Jin et al 2004). On the contrary, the expression of hBD-3 was found in basal cell layer in healthy samples, while in the basal and spinous cell layers in samples from periodontal disease (Lu, Samaranayake et al 2005). The levels of AMPs in GCF are thought to be associated with periodontal disease, as demonstrated by Puklo et al that the GCF HNP1-3 levels were higher in patients with aggressive or chronic periodontitis when compared to healthy controls (Puklo, Guentsch et al 2008).…”

“…How the expression of various AMPs varies during gingival and periodontal inflammation has been reported by various groups, and these studies show high inter-individual variability in both gene and protein expression of AMPs in patient samples (Dunsche, Acil et al 2002;Lu, Jin et al 2004;Dommisch, Acil et al 2005;Lu, Samaranayake et al 2005). Analyses of gene expression by RT-PCR showed hBD-1 and hBD-2 mRNA expression was less frequently detected in tissues with gingivitis than in healthy gingiva.…”

Di̇ş Mobi̇li̇te Tedavi̇si̇nde Güçlendi̇ri̇lmi̇ş Poli̇eti̇len Fi̇bri̇ler Şeri̇t Kullaniminin Di̇ş Eti̇ Oluğu Sividaki̇ HBD-1 Mi̇ktari Üzeri̇ne Etki̇si̇

“…22 Bununla birlikte hBD-1'in aynı zamanda enflame dişeti dokusunda, hBD-2'nin ise sağlıklı durumlarda eksprese edildiğini gösteren bazı araştırmalar da mevcuttur. 24 Bu çalışmada yapılan çalışmalara paralel olarak periodontal hastalığın tedavi edilmesine ilaveten mobilitenin azalması ile beraber hBD-1 düzeyinin azaldığı belirlenmiştir. Bu çalışmada sadece periodontal hastalığın tedavi edildiği ve mobilite tedavisi uygulanmayan kontrol grubu oluşturulmadığı için hBD-1 düzeyindeki azalmaya mobilite tedavisinin ne derece etkisi olduğunu bilinmemektedir.…”

Resposta imune inata na estomatite protética: interação in vitro entre células epiteliais de palato humano e Candida albicans

“…Seis moléculas distintas de hBDs (hBD-1 a -6) foram identificados (HARADA et al, 2004), com os subgrupos hBD-1, hBD-2 e hBD-3 sendo encontrados em epitélio bucal, glândulas salivares, saliva e fluido crevicular gengival (LU et al, 2006;ABIKO et al, 2007). hBD-1 e hBD-2 são intensamente detectadas nas camadas espinhosa, granular e queratinizada do epitélio (DALE et al, 2001), protegendo a mucosa mediante atividade antifúngica direta, além de participarem no recrutamento de células dendríticas e linfócitos T (YANG et al, 1999); enquanto que hBD-3, encontrada principalmente na camada basal do epitélio (LU et al, 2005) albicans e as respostas epiteliais de defesa diante desse microrganismo. Não se tem o conhecimento se os processos de agressão e resposta de defesa epitelial são alterados tanto pelo efeito direto quanto indireto do fungo sobre a célula, e nem ao menos, se tais processos ocorrem ao mesmo tempo ou em tempos distintos.…”