Expression of human liver fatty acid-binding protein in Escherichia coli and comparative analysis of its binding characteristics with muscle fatty acid-binding protein

Maatman, R.; Moerkerk, H.T.B. van; Nooren, Irene; Zoelen, E.J.J. van; Veerkamp, J.H.

doi:10.1016/0005-2760(94)90002-7

Cited by 61 publications

(47 citation statements)

References 45 publications

(34 reference statements)

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“…Although two earlier studies separately examined the LCFA binding profiles of rat [7;9;93] and human [94] wild-type L-FABPs, direct comparisons are difficult since different displacement assays were used in each of these studies and both differed from that presented herein. Both human and rat wild-type L-FABP bound the key ligand substrates for triglyceride synthesis: i) LCFA-CoA, consistent with earlier studies [7;9;93;94]; ii) lysophosphatidic, consistent with earlier studies [93;94]; iii) phosphatidic acid (POPA); and iv) L-FABP binding to monoacylglycerol is controversial depending on the technique used. Gel filtration chromatography of liver cytosol, solution NMR, Lipidex, and binding assay with fluorescent MG analog 12-(9-anthroyloxy)oleoyl- sn -1-glycerol (MG12AO) showed that L-FABP binds MG [95;96].…”

Section: Discussionmentioning

confidence: 99%

Structural and functional interaction of fatty acids with human liver fatty acid‐binding protein (L‐FABP) T94A variant

et al. 2014

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The human liver fatty acid binding protein (L-FABP) T94A variant, the most common in the FABP family, has been associated with elevated liver triglyceride (TG) levels. How this amino acid substitution elicits these effects is not known. This issue was addressed with human recombinant wild-type (WT, T94T) and T94A variant L-FABP proteins as well as cultured primary human hepatocytes expressing the respective proteins (genotyped as TT, TC, and CC). T94A substitution did not or only slightly alter L-FABP binding affinities for saturated, monounsaturated, or polyunsaturated long chain fatty acids (LCFA), nor did it change the affinity for intermediates in TG synthesis. Nevertheless, T94A substitution markedly altered the secondary structural response of L-FABP induced by binding LCFA or intermediates of TG synthesis. Finally, T94A substitution markedly diminished polyunsaturated fatty acid, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), induction of peroxisome proliferator-activated receptor alpha (PPARα) - regulated proteins such as L-FABP, fatty acid transport protein 5 (FATP5), and PPARα itself in cultured primary human hepatocytes. Thus, while T94A substitution did not alter the affinity of human L-FABP for LCFAs, it significantly altered human L-FABP structure and stability as well as conformational and functional response to these ligands.

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Section: Discussionmentioning

confidence: 99%

Structural and functional interaction of fatty acids with human liver fatty acid‐binding protein (L‐FABP) T94A variant

et al. 2014

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“…To our knowledge, no differences between oleate and palmitate binding affinities were reported in the human fatty acid binding proteins (FABPs) studied so far, including liver, muscle (40), and adipose tissue (41). Furthermore, studies that have measured fasting fractional uptake of fatty acids by heart (42), liver, or forearm (43) have failed to demonstrate any difference between palmitate and oleate.…”

Section: Discussionmentioning

confidence: 99%

Physical Inactivity Differentially Alters Dietary Oleate and Palmitate Trafficking

et al. 2009

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1OBJECTIVE-Obesity and diabetes are characterized by the incapacity to use fat as fuel. We hypothesized that this reduced fat oxidation is secondary to a sedentary lifestyle. RESEARCH DESIGN AND METHODS-We investigated the effect of a 2-month bed rest on the dietary oleate and palmitate trafficking in lean women (control group, n ϭ 8) and the effect of concomitant resistance/aerobic exercise training as a countermeasure (exercise group, n ϭ 8). Trafficking of stable isotope-labeled dietary fats was combined with muscle gene expression and magnetic resonance imaging-derived muscle fat content analyses. 31 ]palmitate oxidation by Ϫ8.2 Ϯ 4.9% (P Ͻ 0.0001). Despite a decreased spontaneous energy intake and a reduction of 1.9 Ϯ 0.3 kg (P ϭ 0.001) in fat mass, exercise training did not mitigate these alterations but partially maintained fat-free mass, insulin sensitivity, and total lipid oxidation in fasting and fed states. In both groups, muscle fat content increased by 2.7% after bed rest and negatively correlated with the reduction in [d 31 ]palmitate oxidation (r 2 ϭ 0.48, P ϭ 0.003). I n our search of the environmental factors that fuelled the pandemic of obesity, we face a paradox. Although sedentary lifestyle has been highlighted for decades as one of the main factors triggering weight gain, the physiology of physical inactivity has received little attention (1). Clearly, the causal relationships between sedentary behaviors and obesity are essentially based on epidemiological studies or on the indirect beneficial effects of exercise training (2). None of these studies provide evidence to support a cause-and-effect relationship. RESULTS-In CONCLUSIONS-WhileObesity is a fat storage disease characterized by insulin resistance and a decreased capacity to oxidize lipids (3) in fasting (4) and postprandial (5) conditions. Because weight reduction was not associated with improvement in fat utilization (6), it was suggested as a primary impairment in the etiology of obesity, rather than an adaptive response. Consequently, the delineation of the causes responsible for this reduced capacity to oxidize fat appears to be a fundamental prerequisite to develop efficient strategies against obesity.We previously extended the early Mayer hypothesis (7) and hypothesized that the decreased fat oxidation observed in obese and postobese subjects is due to the generalized adoption of sedentary behaviors (8). Using strict bed rest as a model, we showed that physical inactivity, per se (i.e., independent of the known physical inactivity-induced energy balance changes), lowers fasting and postprandial fat oxidation (9). Unexpectedly, whereas monounsaturated dietary fat (oleate) oxidation remained unaffected by bed rest, saturated fat (palmitate) oxidation decreased by 11% (9). These results are interesting when considering the north/south gradient in obesity prevalence in France that was not associated with the overall energy intake but in the greater amount of saturated fat in the diet (10).The main objective of our present study wa...

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“…Fatty acids are hydrophobic molecules that must be solubilized and transported within the cell by intracellular lipid chaperones known as fatty acid-binding proteins (FABP) 2 (1-4). These low molecular mass (14 -15 kDa) polypeptides are conserved from Caenorhabditis elegans to humans and are involved in fatty acid uptake, transport, and oxidation (5,6). FABPs are abundant in tissues that either require large fluxes of fatty acids or have high demand for fatty acids as an energy source.…”

mentioning

confidence: 99%

Novel Functions of Lipid-binding Protein 5 in Caenorhabditis elegans Fat Metabolism

Joo

Paik

2011

Journal of Biological Chemistry

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The lipid-binding protein (LBP) family is conserved from Caenorhabditis elegans to mammals and essential for fatty acid homeostasis. RNAi-mediated knockdown of nine C. elegans lbp family members revealed that lbp-5 regulates fat accumulation. C. elegans LBP-5 bound directly to various fatty acids with varying affinities. lbp-5 expression in nhr-49(nr2041) worms was much lower than in N2 worms. Nhr-49 transcriptional activity also decreased with lbp-5 deletion, suggesting that they may work together as functional partners in fat metabolism. In support of this notion, LBP-5 translocated into nuclei, where it appeared to influence C. elegans NHR-49 target genes involved in energy metabolism. Interestingly, LBP-5 is required for stearic acid-induced transcription of NHR-49 target genes. Thus, this knowledge could help identify therapeutic targets to treat obesity and diseases associated with nematode-host interactions.

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Expression of human liver fatty acid-binding protein in Escherichia coli and comparative analysis of its binding characteristics with muscle fatty acid-binding protein

Cited by 61 publications

References 45 publications

Structural and functional interaction of fatty acids with human liver fatty acid‐binding protein (L‐FABP) T94A variant

Structural and functional interaction of fatty acids with human liver fatty acid‐binding protein (L‐FABP) T94A variant

Physical Inactivity Differentially Alters Dietary Oleate and Palmitate Trafficking

Novel Functions of Lipid-binding Protein 5 in Caenorhabditis elegans Fat Metabolism

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