Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines

Niemeyer, CM; Sieff, CA; Mathey-Prévôt, Bernard; Wimperis, JZ; Bierer, B E; Clark, SC; Nathan, DG

doi:10.1182/blood.v73.4.945.bloodjournal734945

Cited by 14 publications

(16 citation statements)

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“…Ferrer et al [24] demonstrated that pre-incubating basophils with IL-3 increased the amount of histamine release, induced by sera from chronic urticaria patients. It is of importance to notice that IL-3 gene expression has been demonstrated in activated T cells [25]. The sources of MMP-9 were not identified in this study.…”

Section: Discussionmentioning

confidence: 63%

Increased plasma levels of matrix metalloproteinase‐9 are associated with the severity of chronic urticaria

Kessel

Bishara

Amital

et al. 2005

Clin Experimental Allergy

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Section: Discussionmentioning

confidence: 63%

Increased plasma levels of matrix metalloproteinase‐9 are associated with the severity of chronic urticaria

Kessel

Bishara

Amital

et al. 2005

Clin Experimental Allergy

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“…As CD2 mRNA was also detected it can be surmised that T cells are present in the adherent stroma layer (Niemeyer et a!, 1989) and it raises the question of whether IL-3 production is exclusive to this subpopulation within the stroma. Ongoing studies employing single-cell identification techniques such as in situ hybridization with RNA probes, wili establish the contribution of specific stroma cell types to 1L-3 production.…”

Section: Discussionmentioning

confidence: 99%

IL‐3 is produced by normal stroma in leng‐term bone marrow cultures

Gibson¹,

Scopes²,

Daly³

et al. 1995

Br J Haematol

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Interleukin-3 (IL-3) has been shown to have significant effects on haemopoiesis in vitro, but early investigations of normal human long-term bone marrow cultures (LTBMC) have failed to demonstrate IL-3 production by stromal cells, either by Northern blotting for mRNA, or assaying for bioactivity in culture supernatants. One recent report, using reverse transcription-polymerase chain reaction (RT-PCR), demonstrated IL-3 expression in only one of eight cultures. We have developed a sensitive bioassay for the detection of IL-3 production from normal stroma in LTBMC. LTBMC were grown until confluent, irradiated, and stroma harvested by trypsinization to yield single-cell suspensions. These cells were then cocultured with target bone marrow mononuclear cells (BMMC), or CD34+ cells in clonogenic assays, either in the presence or absence of anti-IL-3 neutralizing antibodies. We have demonstrated IL-3 production in 32/34 cases. In addition, by separating stroma from target cells using cell culture inserts, we have shown that direct stroma:stem cell contact is not necessary for colony growth, suggesting that IL-3 diffuses into the supernatant. However, when supernatants from LTBMC were assayed by enzyme-linked immunoassay (ELISA), no IL-3 was detected. This suggests that IL-3 is probably produced at low levels and has a short-range interaction. Stroma production of IL-3 was confirmed by the detection by RT-PCR of IL-3 mRNA in 3/3 cases. The simultaneous detection of CD2 mRNA demonstrated that T cells are part of the bone marrow stroma. It is therefore possible and probably likely that these cells are the source of IL-3.

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“…This was further supported by the observation that stromal cells remained of host origin following marrow transplantation, even in those patients cured of their disease (Simmons et al, 1987). However, more recent studies suggest that there are non-stromal components of the microenvironment, i.e., monocytes and lymphocytes, which play critical roles in affecting stromal cell function (Vellenga et al, 1988;Niemeyer et al, 1989). Acquired defects within these cell compartments could interfere with the function of the microenvironment, causing marrow failure and resulting in pancytopenia.…”

Section: Discussionmentioning

confidence: 99%

Production of granulocyte colony‐stimulating factor and granulocyte/macrophage‐colony‐stimulating factor after interleukin‐1 stimulation of marrow stromal cell cultures from normal or aplastic anemia donors

Migliaccio

Adamson

et al. 1992

Journal Cellular Physiology

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We have studied stromal cell function in naive or interleukin-1 (IL-1)-stimulated (100 pg/ml) long-term marrow cultures (LTC) from 12 normal donors and 21 patients with severe aplastic anemia (AA). Conditioned media (CM) from normal LTC contained levels of erythroid burst-promoting activity (BPA) and granulocyte/macrophage (GM) colony-stimulating activity (CSA) comparable to those previously described (Migliaccio et al., [1990] Blood, 75:305-312). The addition of IL-1 to these cultures increased the level of CSA and, specifically, of granulocyte colony-stimulating factor (G-CSF) released. Anti-GM-CSF antibody neutralized BPA and CSA in normal naive LTC CM but only the CSA in the CM from IL-1-stimulated LTC. Since the concentrations of GM-CSF, as detected with a specific immunoassay, did not increase after IL-1 treatment, these data suggest that IL-1-stimulated cultures contain an unidentified growth factor having BPA. CM from AA stromal cells contained levels of CSA comparable to those observed in normal stromal cell CM but had significantly lower levels of BPA. Neither anti-GM-CSF nor anti-IL-3 antibodies neutralized the BPA in AA stromal cell CM. This activity may be related to that found in the CM of IL-1-treated normal stromal cells. In nearly 50% of stromal cell cultures of AA patients, addition of IL-1 failed to increase the BPA, CSA, or G-CSF. The presence of an inhibitor in naive or IL-1-treated AA stromal cell CM was excluded by adding the CM to IL-3-stimulated cultures. These findings suggest that G-CSF and GM-CSF genes are differentially regulated in the marrow microenvironment. Furthermore, a marrow microenvironment, deficient in BPA production and, in some cases, unresponsive to IL-1 could contribute to marrow failure in some patients with AA.

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Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines

Cited by 14 publications

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Increased plasma levels of matrix metalloproteinase‐9 are associated with the severity of chronic urticaria

Increased plasma levels of matrix metalloproteinase‐9 are associated with the severity of chronic urticaria

IL‐3 is produced by normal stroma in leng‐term bone marrow cultures

Production of granulocyte colony‐stimulating factor and granulocyte/macrophage‐colony‐stimulating factor after interleukin‐1 stimulation of marrow stromal cell cultures from normal or aplastic anemia donors

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