Expression of human DEC-205 (CD205) multilectin receptor on leukocytes

Abstract

DEC-205 (CD205) belongs to the macrophage mannose receptor family of C-type lectin endocytic receptors and behaves as an antigen uptake/processing receptor for dendritic cells (DC). To investigate DEC-205 tissue distribution in human leukocytes, we generated a series of anti-human DEC-205 monoclonal antibodies (MMRI-5, 6 and 7), which recognized epitopes within the C-type lectin-like domains 1 and 2, and the MMRI-7 immunoprecipitated a single approximately 200 kDa band, identified as DEC-205 by mass spectrometry. MMRI-7 and another DEC-205 mAb (MG38), which recognized the epitope within the DEC-205 cysteine-rich and fibronectin type II domain, were used to examine DEC-205 expression by human leukocytes. Unlike mouse DEC-205, which is reported to have predominant expression on DC, human DEC-205 was detected by flow cytometry at relatively high levels on myeloid blood DC and monocytes, at moderate levels on B lymphocytes and at low levels on NK cells, plasmacytoid blood DC and T lymphocytes. MMRI-7 F(ab')2 also labeled monocytes, B lymphocytes and NK cells similarly excluding reactivity due to non-specific binding of the mAb to FcgammaR. Tonsil mononuclear cells showed a similar distribution of DEC-205 staining on the leukocytes. DEC-205-specific semiquantitative immunoprecipitation/western blot and quantitative reverse transcriptase-PCR analysis established that these leukocyte populations expressed DEC-205 protein and the cognate mRNA. Thus, human DEC-205 is expressed on more leukocyte populations than that were previously assumed based on mouse DEC-205 tissue localization studies. The broader DEC-205 tissue expression in man is relevant to clinical DC targeting strategies and DEC-205 functional studies.

Dendritic cells are abundant in non-lesional gray matter in multiple sclerosis

“…CD205 is also expressed by CD8 + DCs that are believed to play a role in cross priming (Kato et al, 2006). We found CD205 positive cells mainly in perivascular cuffs of NLGM, NLWM and MS plaques (Fig.…”

“…Fewer perivascular DCs expressing CD205 and CD83 makers for the mature cells were found compared to the CD209 + DCs. Since CD205 is also expressed by CD8 + DCs it is possible that these perivascular CD205 + cells play a role in CD8 + T cells priming (Kato et al, 2006).…”

“…Further, we investigated if DCs in MS brain also express CD205; CD205 is highly expressed by mature DCs, belong to the macrophage mannose receptor family of C-type lectin endocytic receptors and is involved in antigen uptake and processing (Kato et al, 2006). CD205 is also expressed by CD8 + DCs that are believed to play a role in cross priming (Kato et al, 2006).…”

Antibody-targeted vaccines

“…DEC-205 is a CLR belonging to the MR family (Kato et al, 2006). DEC-205 contains 10 CRDs for which the specific ligands remain to be identified.…”

“…DEC-205 contains 10 CRDs for which the specific ligands remain to be identified. The human and murine DEC-205 share 77% identity, and their expression is primarily restricted to DCs and thymic cortical epithelium, although human DEC-205 can also be detected on peripheral T and B cells (Kato et al, 2006). A distinguishing feature of DEC-205 is its highlevel expression on DCs in the T-cell areas of lymphoid organs, suggesting a role in regulating T-cell responses.…”