Expression of hsp90 mediates cytoprotective effects in the gastrodermis of planarians

Conte, Maria; Isolani, Maria Emilia; Deri, Paolo; Mannini, Linda; Batistoni, Renata

doi:10.1007/s12192-010-0218-6

Cited by 15 publications

(8 citation statements)

References 48 publications

(46 reference statements)

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“…The presence of Djhsp90 transcripts in the intestine appears to sustain this role ( Fig. 4A,B; Conte et al, 2011). However, expression of Djhsp90 was also detected in the parenchyma of unstressed animals, localized in small neoblastlike cells (Fig.…”

Section: Hsp90 Genesmentioning

confidence: 83%

“…We recently reported the first characterization of an hsp90-related gene (Djhsp90) in the planarian D. japonica (Conte et al, 2011). The dynamics of changes in the expression profile in response to various stress conditions clearly demonstrates that Djhsp90 encodes a stress-induced protein.…”

Section: Hsp90 Genesmentioning

confidence: 99%

“…4C). Although Djhsp90 expression may be limited only to neoblasts committed towards the gastrodermal fate (Conte et al, 2011), the multiplicity of roles assigned to HSP90 proteins supports the possibility that DjHSP90 plays different function(s) in planarian stem cells, possibly influencing signal transduction and/or chromatin remodelling mechanisms. Further studies are necessary to identify the relationships between DJHSP90 and planarian stem cells.…”

Section: Hsp90 Genesmentioning

confidence: 99%

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Stem cell protection mechanisms in planarians: the role of some heat shock genes

Isolani¹,

Conte²,

Deri³

et al. 2012

Int. J. Dev. Biol.

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Planarians contain a large population of stem cells, named neoblasts, and use these for continuous turnover of all cell types. In addition, thanks to the amazing flexibility of these cells, planarians respond well to the effects of stressful situations, for example activating regeneration after trauma. How neoblasts respond to stress and support continuous proliferation, maintaining long-term stability, is still an open question. Heat shock proteins (HSPs) are a complex protein family with key roles in maintaining protein homeostasis, as well as in apoptosis and growthrelated processes. We recently characterized some planarian homologs of hsp genes that are highly expressed in mammalian stem cells, and observed that some of them are critical for neoblast survival/maintenance. The results of these studies support the notion that some HSPs play crucial roles in the modulation of pathways regulating stem cell activity, regeneration and tissue repair. In this review we compare the evidence available for planarian hsp genes and focus on questions emerging from these results.

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Section: Hsp90 Genesmentioning

confidence: 83%

Section: Hsp90 Genesmentioning

confidence: 99%

Section: Hsp90 Genesmentioning

confidence: 99%

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Stem cell protection mechanisms in planarians: the role of some heat shock genes

Isolani¹,

Conte²,

Deri³

et al. 2012

Int. J. Dev. Biol.

Self Cite

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“…An increase in HSPs was already demonstrated in flatworms after exposure to other toxicants such as copper and magnesium chloride (Guecheva et al, ; Navarro et al, ). Candidates belonging to the HSP class of proteins are associated with stem cell biology and are involved in the neoblast metabolism of flatworms (Pfister et al, ; Altincicek and Vilcinskas, ; Eisenhoffer et al, ; Conte et al, , Conte et al, , Conte et al, , Fernandez Taboada et al, ; Ma et al, ). The ultrastructure of the neoblasts was barely affected after 24 h of exposure, even when exposed to the high concentration of 250 μM CdCl 2 .…”

Section: Discussionmentioning

confidence: 99%

Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms

et al. 2015

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Stem cells or undifferentiated cells can cope more easily with external stresses. To evaluate the impact of toxic compounds on stem cell dynamics in vivo, in relation to other biological responses, we use the carcinogenic element cadmium and the regenerating model organism Macrostomum lignano. Through both BrdU and anti-histone H3 immunostainings, cadmium-induced effects were investigated at different stages of the stem cell cycle. A 24-h exposure to 100 and 250 μM CdCl2 significantly decreased the number of stem cells (neoblasts) in mitosis, whereas the number of cells in the S phase remained unchanged. After this short-term exposure, the ultrastructure of the neoblasts was minimally affected in contrast to the epidermal tissues. These results were supported by gene expression data: transcripts of cdc2 and pig3 were significantly upregulated during all treatments. Both genes are involved in the cell cycle progression and are transcribed in the gonadal region, where stem cells are highly represented. Based on a substantial increase in gene expression of heat shock proteins (HSP) and their high activity in the gonadal region, we hypothesize that these proteins are key players in the protection of stem cells against external stresses. Apart from the strong HSP induction, other protective processes including cell division, apoptosis and anti-oxidative defence, were also activated. We, therefore, conclude that the protection of stem cells against external stressors may be based on the interplay between stem cell maintenance, i.e. repair and recovery through division, on one hand and apoptosis on the other hand. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1217-1228, 2016.

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“… All phenotypes characterized that cause a loss or reduction in blastema formation (not associated with a known polarity defect) and/or ventral curling and lysis are listed in alphabetical order for each species 39–41,57,84,87,90,94,96,203,205,209,217,223–242. Reported data regarding numbers of neoblasts, cell proliferation based on Phospho‐Histone H3 (Ser10) staining, expression of early and late neoblast progeny markers,90 results from clonogenic expansion assays,240 and miscellaneous phenotype data are included. …”

Section: Neoblasts: Cellular Agents Of Planarian Regenerationmentioning

confidence: 99%

The history and enduring contributions of planarians to the study of animal regeneration

Elliott

Alvarado

2012

WIREs Developmental Biology

183

161

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Having an almost unlimited capacity to regenerate tissues lost to age and injury, planarians have long fascinated naturalists. In the Western hemisphere alone, their documented history spans more than 200 years. Planarians were described in the early 19th century as being “immortal under the edge of the knife,” and initial investigation of these remarkable animals was significantly influenced by studies of regeneration in other organisms and from the flourishing field of experimental embryology in the late 19th and early 20th centuries. This review strives to place the study of planarian regeneration into a broader historical context by focusing on the significance and evolution of knowledge in this field. It also synthesizes our current molecular understanding of the mechanisms of planarian regeneration uncovered since this animal’s relatively recent entrance into the molecular-genetic age.

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Expression of hsp90 mediates cytoprotective effects in the gastrodermis of planarians

Cited by 15 publications

References 48 publications

Stem cell protection mechanisms in planarians: the role of some heat shock genes

Stem cell protection mechanisms in planarians: the role of some heat shock genes

Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms

The history and enduring contributions of planarians to the study of animal regeneration

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