Expression of HOX homeogenes in human neuroblastoma cell culture lines

Peverali, Fiorenzo A.; D’Esposito, Maurizio; Acampora, Dario; Bunone, Giuseppe; Negri, Mario; Faiella, A.; Stornaiuolo, Anna; Pannese, Maria; Migliaccio, Enrica; Simeone, Antonio; Valle, Giuliano Della; Boncinelli, Edoardo

doi:10.1111/j.1432-0436.1990.tb00458.x

Cited by 36 publications

(33 citation statements)

References 36 publications

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“…2 B ). It is of interest that this sequence shares 50% identity with the COOH-terminal tail of the human homeobox protein HoxB4 (SGGAAGSAGGPPGRPPNGGPRAL; underlined are the identical sequences between the two proteins) (12).…”

Section: Resultsmentioning

confidence: 99%

A novel variant of human Grb7 is associated with invasive esophageal carcinoma.

Tanaka

Mori²,

Akiyoshi

et al. 1998

J. Clin. Invest.

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The cDNAs of a putative growth factor-bound (Grb) 7 signal transduction molecule and Grb7V novel splice variant were isolated from an invasive human esophageal carcinoma. Although both Grb7 isoforms share homology with the Mig-10 cell migration gene, the Grb7V isoform lacks 88 base pairs in the C terminus; the resultant frame shift leads to substitution of an SH2 domain with a short hydrophobic sequence. The wild-type Grb7 protein, but not the Grb7V isoform, is rapidly tyrosyl phosphorylated in response to EGF stimulation in esophageal carcinoma cells. Analysis of human esophageal tumor tissues and regional lymph nodes with metastases revealed that Grb7V was expressed in 40% of Grb7-positive esophageal carcinomas. More importantly, Grb7V expression was enhanced after metastatic spread to lymph nodes as compared to the original tumor tissues.

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Section: Resultsmentioning

confidence: 99%

A novel variant of human Grb7 is associated with invasive esophageal carcinoma.

Tanaka

Mori²,

Akiyoshi

et al. 1998

J. Clin. Invest.

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“…and (3) Do alternatively spliced forms of the HOX homeodomain proteins account for the observed differences in subcellular distribution? For HOXB4, several different sized mRNA transcripts have been reported but only a single protein coding sequence has been described (Peverali et al, 1990) and its relationship to the various transcripts is unknown. Questions concerning the possible roles of cytoplasmic HOX proteins or possible regulation of the movement of HOX proteins from the cytoplasm to the nucleus remain almost completely unanswered.…”

Section: Discussionmentioning

confidence: 99%

“…A HOXB4 cDNA encoding a full-length, homeodomain containing protein (Peverali et al, 1990) was subcloned into the MSCVneoEB retroviral vector such that expression of the cDNA is under control of the retroviral 5Ј long terminal repeat (Sauvageau et al, 1995). Plasmid DNA was transfected by calcium phosphate precipitation into GϩPE cells, and virus containing supernatants were collected 24 and 48 hours after transfection.…”

Section: Retroviral Infection Of Keratinocytesmentioning

confidence: 99%

HOXB4 homeodomain protein is expressed in developing epidermis and skin disorders and modulates keratinocyte proliferation

Kömüves

Michael

Arbeit

et al. 2002

Developmental Dynamics

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The HOX homeodomain proteins are fundamental regulators of organ and tissue development, where they are thought to function as transcription factors, and HOX gene expression has been associated with numerous types of cancers. Previous studies have demonstrated that enforced expression of the HOXB4 protein transforms cultured fibroblasts and leads to a selective expansion of the hematopoietic stem cell pool, suggesting that this protein might play a role in cellular proliferation. In support of this concept, we now show that enforced expression of HOXB4 in human neonatal keratinocytes results in increased cellular proliferation and colony formation as well as decreased expression of the alpha-2-integrin and CD44 cell surface adhesion molecules. We previously have reported HOXB4 gene expression in the basal and suprabasal layers of developing human skin and now show extensive HOXB4 mRNA in psoriatic skin and basal cell carcinoma. In fetal human skin HOXB4 protein expression was both nuclear and cytoplasmic within epidermal basal cells and in hair follicle inner and outer root sheath cells, whereas strong nuclear signals were observed in the bulge region. In adult skin, HOXB4 protein expression was both nuclear and cytoplasmic, but was predominantly localized to the intermediate and differentiated cell layers. In contrast to the striking gradient patterns of HOX gene and protein expression previously described in developing spinal cord and limb, HOXB4 protein was uniformly detected in all regions of the fetal and adult skin. Although little HOXB4 signal localized to proliferative cell layers, as marked by proliferating cell nuclear antigen (PCNA) staining, in normal adult epidermis, nuclear HOXB4 protein expression substantially overlapped with PCNA-positive cell in a series of samples of hyperproliferative skin. Taken together, these data suggest that nuclear HOXB4 protein may play a role in the regulation of cellular proliferation/adhesion in developing fetal human epidermis and in hyperproliferation conditions, including cancers, in adult epidermis.Published 2002 Wiley-Liss, Inc. †

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“…By the way of above, HOX genes can affect genes transcription by specifying the positional identities of chromosomes [43]. Aberrant expressions of HOX genes may affect cells differentiation and ontogeny, causing abnormal tissues and organs, and even contribute to a variety of tumors, such as ovarian cancer [44], gastric cancer [45], colorectal cancer [46,47], neuroblastoma [48], kidney cancer [49], prostate cancers [50] and hematological malignancies [51,52,53].…”

Section: Homeobox Genesmentioning

confidence: 99%

HOTAIR: a cancer-related long non-coding RNA

Cai¹,

Song²,

Cai³

et al. 2014

neo

149

126

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Long non-coding RNA was dismissed as merely transcriptional "noise" in the past decades. Numerous researches have shown that lncRNAs regulated gene expression at the epigenetic level. Moreover, lncRNAs played important roles in proliferation, apoptosis and invasiveness of tumor cells, and participated in metastatic capacity of cancers. Recent studies revealed HOX transcript antisense RNA, a lncRNA with regulatory functions of transcription, could bind PRC2 and LSD1/CoREST/REST complexes and direct to the specific gene sites, resulted in H3K27 methylation and H3K4 demethylation and ultimately gene silencing. Aberrant HOTAIR expression was associated with various sites of cancers such as breast, hepatocellular, gastric, colorectal, pancreatic et al; and affected survival and prognosis of cancer patients. In this review, we introduce an overall view of HOTAIR by describing the known molecular mechanisms and potential functions of HOTAIR and summarizing the latest progresses on the research of HOTAIR in various human cancers.Key words: HOTAIR, LncRNA, HOX genes, cancer Long non-coding RNAs (LncRNAs) are commonly defined as RNA moleculars larger than 200 nucleotides. LncRNA was first found among transcribed DNA product of the mouse by Okazaki [1]. Many identified lncRNAs were transcribed by RNA polymerase II (RNA pol II) and spliced [2,3]. One view existed in the past few decades that lncRNAs were as diverse as their better known counterpart messenger RNAs (mRNAs), having no protein-coding capacity, were described as transcriptional "noise" [4,5,6]. However, a number of studies have shown that some lncRNAs were involved in embryogenesis and differentiation [7,8]. These lncRNAs are expressed in specific cell types [9, 10, 11] and located in specific subcellular compartments [12,13,14]. Moreover, recent studies have indicated that lncRNAs participated in a wide range of biological pathways and cellular processes. They could regulate gene expression and function, including dosage compensation [15,16] [18,36].HOX transcript antisense RNA (HOTAIR) is a lncRNA which has regulatory functions of transcription and are transcribed from the antisense strand of homeobox C gene locus in chromosome 12. HOTAIR recruits Polycomb Repressive Complex 2 (PRC2) and histone demethylase complex [LSD1 (lysine specific demethylase 1)/CoREST (Co-repressor of RE1-silencing transcription factor)/REST]; then leads to histone H3 tri-methylated at lysine 27 (H3K27me3) and histone H3 dimethyl Lys4 (H3K4me2); consequently results in gene silencing. In addition to its scaffold function to assemble transcription regulators, and a latest study reported that HO-TAIR could also serve as a platform to control protein levels via the ubiquitin-proteasome pathway. HOTAIR facilitated the ubiquitination of Ataxin-1 by Dzip3 and Snurportin-1 by Mex3b, and then accelerated their degradation. Moreover, HOTAIR levels were highly upregulated in senescent cells. It

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Expression of HOX homeogenes in human neuroblastoma cell culture lines

Cited by 36 publications

References 36 publications

A novel variant of human Grb7 is associated with invasive esophageal carcinoma.

A novel variant of human Grb7 is associated with invasive esophageal carcinoma.

HOXB4 homeodomain protein is expressed in developing epidermis and skin disorders and modulates keratinocyte proliferation

HOTAIR: a cancer-related long non-coding RNA

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Expression of HOX homeogenes in human neuroblastoma cell culture lines

Cited by 36 publications

References 36 publications

A novel variant of human Grb7 is associated with invasive esophageal carcinoma.

A novel variant of human Grb7 is associated with invasive esophageal carcinoma.

HOXB4 homeodomain protein is expressed in developing epidermis and skin disorders and modulates keratinocyte proliferation

HOTAIR: a cancer-related long non-coding RNA

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HOTAIR: a cancer-related long non-coding RNA