Expression of HOTAIR and MEG3 are negatively associated with H. pylori positive status in gastric cancer patients

Amini, Farnaz; Khalaj‐Kondori, Mohammad; Moqadami, Amin; Rajabi, Ali

doi:10.18632/genesandcancer.219

Cited by 8 publications

(4 citation statements)

References 33 publications

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“…It is challenging to achieve advancements in traditional approaches regarding the side effects of chemotherapies and molecular features of the tumor cells (17). As a result, lncRNAs have been identified as beneficial indicators for cancer onset and cell growth (9,18). Comprehending the role of lncRNAs in cancer biology could be helpful for the treatment, prognosis, and diagnosis of cancer (7,19,20).…”

Section: Discussionmentioning

confidence: 99%

lncRNA VLDLR-AS1 Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran

Moqadami,

Ahmadi,

Khalaj-Kondori

2023

Jentashapir J Cell Mol Biol

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Background: Colorectal cancer (CRC) is the third most common cancer that frequently spreads to other parts of the body, with a low chance of recovery and a high mortality rate. Long non-coding RNAs are considered significant prognostic and diagnostic indicators because they are dysregulated in various cancers and have distinctive expression patterns and high tissue- and cell-specificity. VLDLR-AS1 lncRNA deregulation has been associated with several malignancies. Objectives: This study aimed to evaluate the expression levels of VLDLR-AS1 in CRC. It was the first time this assessment was conducted. Methods: We studied 188 samples, including 94 tumor samples and 94 paired adjacent non-tumor tissues. Total RNA was extracted, and its quantity and purity were assessed. TaKaRa PrimeScript 1st Strand cDNA Synthesis Kit (Kusatsu, Japan) was used for the reaction. The StepOnePlus Real-Time PCR System (Applied Biosystems) was set up to assess the relative expression of VLDLR-AS1. Results: According to the qRT-PCR data, the VLDLR-AS1 expression levels in CRC tissues were significantly lower than in tumor margins (P < 0.0001). In addition, receiver operating characteristic curve analysis demonstrated that VLDLR-AS1 expression can discriminate between tumor and non-tumor samples with the sensitivity and specificity of 72.34% and 51.06%, respectively (P = 0.03, AUC = 0.6274). However, no significant association was found between the expression levels of VLDLR-AS1 and clinicopathological features in CRC patients. Conclusions: The results of this study indicated that VLDLR-AS1 lncRNA is significantly downregulated in the tumor tissues of CRC patients compared to healthy tumor margin tissues. This evidence shows the potential of this gene as a promising biomarker for the early detection of CRC development.

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Section: Discussionmentioning

confidence: 99%

lncRNA VLDLR-AS1 Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran

Moqadami,

Ahmadi,

Khalaj-Kondori

2023

Jentashapir J Cell Mol Biol

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“…Ali Rajabi et al reported that lncRNA HOXA-AS2 was elevated significantly in cancerous tissues and related to HP infection [ 6 ]. Furthermore, lncRMA HOTAIR, FOXD2-AS1, AF147447 and OC1 play important role in HP infection associated GC [ 7 – 10 ]. In addition, miRNAs plays a significant role in HP- infected GC cells [ 11 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

CircRNA_15430 reduced by Helicobacter pylori infection and suppressed gastric cancer progression via miR-382-5p/ZCCHC14 axis

Liu,

Cao,

Yang

et al. 2023

Biol Direct

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Background Helicobacter pylori (H.pylori, HP) is one of the main causes of gastric cancer (GC). CircRNAs have been reported to play a crucial role in developing many types of cancer. However, the role of circRNAs in the development and progression of HP infected-GC has not been studied. Methods The location of circRNA_15430 in GC cells were detected by nuclear and cytoplasmic RNA fractionation and RNA fluorescence in situ hybridization analysis (FISH) assays, and circRNA_15430, miR-382-5p and ZCCHC14 expression in GC cell lines and tissues were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The function of circRNA_15430 in GC cells were examined by using colony formation, cell counting kit-8 (CCK-8) and Transwell assays, flow cytometry and laser scanning confocal microscopy. The protein levels were detected by Western blotting. Whether circRNA_15430 sponges miR-382-5p was monitored with a dual-luciferase reporter assay. Furthermore, circRNA_15430 was analyzed in vivo in tumor growth with nude mice. Results CircRNA_15430 is primarily localized in the cytoplasm of GC cells, and downregulated in the GC cell lines and tissues, and is negatively correlated with the tumor size. Downregulation of circRNA_15430 promotes proliferation, migration and suppresses cell apoptosis and autophagy in GC cells. Mechanically, circRNA_15430 acts as a miR-382-5p sponge, alleviating the inhibitory effect of miR-382-5p on its target ZCCHC14. Knockdown circRNA_15430 enhances tumor growth in vivo. In addition, circRNA_15430 was reduced in HP + gastritis tissues and HP-infected MGC-803 cells, reversing the pro-HP effect on autophagy. Additionally, miR-382-5p was increased in HP + gastritis tissue and HP-infected MGC-803 cells while ZCCHC14 decreased in HP-infected MGC-803 cells. MiR-382-5p reverses the effect of si-ZCCHC14 on autophagosome numbers in MGC-803 cells. Conclusions Therefore, circRNA_15430 plays an inhibitory role in GC and regulates the progression of HP infection-related GC, providing a novel molecular marker for GC therapy.

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“…Maternally expressed gene 3 (MEG3), despite limited expression in various tumors, functions as a tumor suppressor gene in ovarian cancer [ 3 ]. Although the roles of MEG3 in the context of other cancers have been reported earlier [ 4 ], the effects of MEG3 in ODMECs have not been well elucidated. Long noncoding RNAs (lncRNAs) are at least 200 nt transcripts lacking protein-coding potential.…”

Section: Introductionmentioning

confidence: 99%

MEG3 sponges miRNA-376a and YBX1 to regulate angiogenesis in ovarian cancer endothelial cells

Zhang²,

Zhao³

et al. 2023

Heliyon

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Expression of HOTAIR and MEG3 are negatively associated with H. pylori positive status in gastric cancer patients

Cited by 8 publications

References 33 publications

lncRNA VLDLR-AS1 Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran

lncRNA VLDLR-AS1 Gene Expression in Colorectal Cancer in Patients from East Azerbaijan Province, Iran

CircRNA_15430 reduced by Helicobacter pylori infection and suppressed gastric cancer progression via miR-382-5p/ZCCHC14 axis

MEG3 sponges miRNA-376a and YBX1 to regulate angiogenesis in ovarian cancer endothelial cells

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