Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle

Langfort, Józef; Ploug, Thorkil; Ihlemann, Jacob; SALDO, Michele; Holm, Cecilia; Galbo, H.

doi:10.1042/0264-6021:3400459

Cited by 53 publications

(101 citation statements)

References 28 publications

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“…HSL assays are based on measurements of release of [ 3 H]oleic acid from 1(3)-mono[ 3 H]oleoyl¡2-oleylglycerol (a diacylglycerol analogue not hydrolysable at position 2), referred to as HSL (DG) activity, and from tri[ 3 H]olein, referred to as HSL (TG) activity, under conditions optimal for HSL. In the basal state, the catalytic activity of adipose tissue HSL towards diacylglycerol (DG) substrates is about ten-fold higher than the activity against triacylglycerol (TG) substrates Langfort et al 1999;Straalfors et al 1987). Upon phosphorylation by protein kinase A, the activity of adipose tissue and soleus muscle HSL towards TG increases markedly, whereas the HSL (DG) activity does not change signiWcantly Langfort et al 1999;Straalfors et al 1987).…”

Section: Analytical Proceduresmentioning

confidence: 98%

“…Upon phosphorylation by protein kinase A, the activity of adipose tissue and soleus muscle HSL towards TG increases markedly, whereas the HSL (DG) activity does not change signiWcantly Langfort et al 1999;Straalfors et al 1987). Furthermore, the increases in muscle HSL (TG) activity induced by adrenaline or contractions can be completely abolished by an anti-HSL antibody present during analysis (Langfort et al 1999(Langfort et al , 2000. In addition, also the increase in human muscle HSL (TG) activity induced by exercise can be totally abolished by an anti-HSL antibody present during analysis (RoepstorV et al 2004).…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…In the basal state, the catalytic activity of adipose tissue HSL towards diacylglycerol (DG) substrates is about ten-fold higher than the activity against triacylglycerol (TG) substrates Langfort et al 1999;Straalfors et al 1987). Upon phosphorylation by protein kinase A, the activity of adipose tissue and soleus muscle HSL towards TG increases markedly, whereas the HSL (DG) activity does not change signiWcantly Langfort et al 1999;Straalfors et al 1987). Furthermore, the increases in muscle HSL (TG) activity induced by adrenaline or contractions can be completely abolished by an anti-HSL antibody present during analysis (Langfort et al 1999(Langfort et al , 2000.…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…Hormone-sensitive lipase (HSL) is thought to be the most important lipase in human skeletal muscle, and the HSL activity is increased both by adrenaline stimulation and muscle contraction (Langfort et al 1999(Langfort et al , 2000Donsmark et al 2003;Watt et al 2003b). In a series of studies, Watt et al (2003a) colleagues found that the muscle HSL activity was increased after 1 h, but had returned to base line after 4 h of cycle exercise.…”

Section: Introductionmentioning

confidence: 95%

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Muscle triacylglycerol and hormone-sensitive lipase activity in untrained and trained human muscles

Helge¹,

Biba

Galbo³

et al. 2006

Eur J Appl Physiol

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During exercise, triacylglycerol (TG) is recruited in skeletal muscles. We hypothesized that both muscle hormone-sensitive lipase (HSL) activity and TG recruitment would be higher in trained than in untrained subjects in response to prolonged exercise. Healthy male subjects (26 +/- 1 years, body moss index 23.3 +/- 0.5 kg m(-2)), either untrained (N = 8, VO(2max) 3.8 +/- 0.2 l min(-1)) or trained (N = 8, VO(2max) 5.1 +/- 0.1 l min(-1)), were studied. Before and after 3-h exercise (58 +/- 1% VO(2max)), a biopsy was taken. Muscle citrate synthase (32 +/- 2 vs. 47 +/- 6 mumol g(-1) min(-1) d.w.) and beta-hydroxy-acyl-CoA-dehydrogenase (38 +/- 3 vs. 52 +/- 5 mumol g(-1) min(-1) d.w.) activities were lower in untrained than in trained subjects (p < 0.05). Throughout the exercise, fat oxidation was higher in trained than in untrained subjects (p < 0.05). Muscle HSL activity was similar at rest (0.72 +/- 0.08 and 0.74 +/- 0.03 mU mg(-1) protein) and after exercise (0.71 +/- 0.1 and 0.68 +/- 0.03 mU mg(-1) protein) in untrained and trained subjects. At rest, the chemically determined muscle TG content (37 +/- 8 and 26 +/- 5 mmol g(-1) d.w.) was similar (p > 0.05), and after exercise it was unchanged in untrained and lower (p < 0.05) in trained subjects (41 +/- 9 and 10 +/- 2 mmol g((1) d.w.). Determined histochemically, TG was decreased (p < 0.05) after exercise in type I and II fibres. Depletion of TG was not different between fibre types in untrained, but tended to be higher (p = 0.07) in type I compared with type II fibres in trained muscles. In conclusion, HSL activity is similar in untrained and trained skeletal muscles both before and after prolonged exercise. However, the tendency to higher muscle TG recruitment during exercise in the trained subjects suggests a difference in the regulation of HSL or other lipases during exercise in trained compared with untrained subjects.

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Section: Analytical Proceduresmentioning

confidence: 98%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Muscle triacylglycerol and hormone-sensitive lipase activity in untrained and trained human muscles

Helge¹,

Biba

Galbo³

et al. 2006

Eur J Appl Physiol

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“…HSL is most active in adipose tissue. In steroidogenic tissues including the adrenal cortex, the enzyme is about three times less active, and its activity in pancreatic b-cells is even lower, while in the heart and macrophages, it is negligible (Khoo et al 1993, Langfort et al 1999, Lindvall et al 2004.…”

Section: Introductionmentioning

confidence: 99%

Transcription of LIPE gene encoding hormone-sensitive lipase/cholesteryl esterase is regulated by SF-1 in human adrenocortical cells: involvement of protein kinase A signal transduction pathway

Hołysz¹,

Derebecka-Holysz²,

Trzeciak³

2010

Journal of Molecular Endocrinology

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The study was designed to elucidate the influence of the protein kinase A (PKA) signal transduction pathway on transcription of the LIPE gene encoding hormone-sensitive lipase/cholesteryl esterase (HSL) in H295R cells. HSL is one of the key enzymes involved in steroid hormone synthesis, and ACTH, with mediation of the PKA pathway, increases its activity. However, the mode of regulation of LIPE gene expression by ACTH remains unknown. It was found that stimulation of the PKA pathway by the adenylyl cyclase activator, forskolin, caused a twofold increase in LIPE transcript accompanied by appreciable rise in the protein product of the gene and cortisol output. RNA polymerase II inhibitor abolished, and protein synthesis inhibitor attenuated this effect. Forskolin and PKA catalytic subunit increased transcriptional activity of LIPE promoter A in cells transfected with the luciferase reporter vector. Overexpression of steroidogenic factor-1 (SF-1) increased LIPE promoter activity, while transient silencing of SF-1 expression with specific siRNAs abolished forskolin-stimulated LIPE transcription. It is concluded that ACTH via the PKA pathway stimulates expression of SF-1, which activates transcription of LIPE presumably by interaction with putative binding sequences within promoter A. A novel mechanism contributing to the long-term effect of ACTH on adrenal steroidogenesis is proposed: ACTH stimulates transcription of SF-1, which interacts with the putative SF-1-binding sequences within the promoter and activates LIPE transcription. An increased level of HSL results in an enhanced supply of cholesterol required for steroid hormone synthesis.

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Physiological and Pharmacological Regulation of Triacylglycerol Storage and Mobilization

Müller¹,

Petry²

2004

Lipases and Phospholipases in Drug Development

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Expression of hormone-sensitive lipase and its regulation by adrenaline in skeletal muscle

Cited by 53 publications

References 28 publications

Muscle triacylglycerol and hormone-sensitive lipase activity in untrained and trained human muscles

Muscle triacylglycerol and hormone-sensitive lipase activity in untrained and trained human muscles

Transcription of LIPE gene encoding hormone-sensitive lipase/cholesteryl esterase is regulated by SF-1 in human adrenocortical cells: involvement of protein kinase A signal transduction pathway

Physiological and Pharmacological Regulation of Triacylglycerol Storage and Mobilization

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