Expression of HLA-DP0401 Molecules for Identification of DP0401 Restricted Antigen Specific T Cells

Yang, Junbao; Huston, L.; Berger, DeAnna; Danke, Nancy A.; Liu, Andrew W.; Disis, Mary L.; Kwok, William W.

doi:10.1007/s10875-005-6095-6

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…HLA‐DPB1 * 0401 is not in linkage disequilibrium with either DR or DQ alleles and it would therefore be present in individuals with a range of HLA‐DR alleles [48]. HLA class II tetramers have been successfully made for HLA‐DPB1 * 0401 [49] and thus the HLA‐DPB1 * 0401/Fel d 1 epitope identified in this paper offers the potential to accurately establish frequency and phenotype of these T cells. It also offers the potential to monitor alterations in antigen specific T cell frequency and phenotype with changes in disease severity or treatment.…”

Section: Discussionmentioning

confidence: 99%

Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes

Bateman

Ardern‐Jones

Ogg

2008

Clin Experimental Allergy

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Section: Discussionmentioning

confidence: 99%

Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes

Bateman

Ardern‐Jones

Ogg

2008

Clin Experimental Allergy

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“…The following class II monomers and multimers were produced for this study: DRA1/DRB1*0101 (DR0101), DRA1/ DRB1*0301 (DR0301), DR0401, DRA1/DRB1*0404 (DR0404), DRA1/DRB1*0701 (DR0701), DR1101, DR1501, DRA1/DRB5*0101 (DRB5), DQA1*0102/DRB1*0602 (DQ0602) and DPA1*0103/DPB1*0401 (DP0401). Production of these molecules has been previously described (11)(12)(13)(14)(15). Briefly, soluble class II molecules were purified from the supernatants of insect cell cultures and biotinylated using biotin ligase enzyme (Avidity, Denver, CO, USA).…”

Section: Production Of Class II Tetramersmentioning

confidence: 99%

Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition

James

Bui

Berger

et al. 2007

International Immunology

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Tetanus toxoid is a routine positive control antigen for cellular assays. Previous studies identified multiple tetanus toxin (TT) epitopes, including some 'universal' epitopes. However, rigorous HLA-restricted study of tetanus toxoid responses is still lacking. In this study, the tetramer-guided epitope mapping approach was used to identify CD4+ T-cell epitopes within the TT heavy chain restricted by 10 different class II alleles. Of 106 peptides tested, 52 contained epitopes. Response frequencies toward specific epitopes varied, indicating prevalent and rare specificities. Most antigenic peptides (85%) were presented by one or two class II alleles. For peptides presented by three or more alleles, truncation studies revealed that some contained multiple epitopes. These results contrast with the perceived notion that tetanus toxoid responses are dominated by universal CD4+ T-cell epitopes. Rather these results illustrate heterogeneous T-cell responses for different class II alleles and individual-specific variation of the T-cell repertoire.

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“…This low expression level also makes DP more difficult to isolate, purify, and characterize, which may have limited the number of studies conducted with these molecules. However, a growing body of literature indicates that DP encodes fully functional molecules, restricting epitope responses in the context of cancer, allergy, and infectious disease (31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44).…”

mentioning

confidence: 99%

Five HLA-DP Molecules Frequently Expressed in the Worldwide Human Population Share a Common HLA Supertypic Binding Specificity

Sidney

Steen

Moore

et al. 2010

The Journal of Immunology

121

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Compared with DR and DQ, knowledge of the binding repertoires and specificities of HLA-DP alleles is somewhat limited. However, a growing body of literature has indicated the importance of DP-restricted responses in the context of cancer, allergy, and infectious disease. In the current study, we developed high-throughput binding assays for the five most common HLA-DPB1 alleles in the general worldwide population. Using these assays on a comprehensive panel of single-substitution analogs and large peptide libraries, we derived novel detailed binding motifs for DPB1*0101 and DPB1*0501. We also derived more detailed quantitative motifs for DPB1*0201, DPB1*0401, and DPB1*0402, which were previously characterized on the basis of sets of eluted ligands and/or limited sets of substituted peptides. Unexpectedly, all five DP molecules, originally selected only on the basis of their frequency in human populations, were found to share largely overlapping peptide motifs. Testing panels of known DP epitopes and a panel of peptides spanning a set of Phleum pratense Ags revealed that these molecules also share largely overlapping peptide-binding repertoires. This demonstrates that a previously hypothesized DP supertype extends far beyond what was originally envisioned and includes at least three additional very common DP specificities. Taken together, these DP supertype molecules are found in >90% of the human population. Thus, these findings have important implications for epitope-identification studies and monitoring of human class II-restricted immune responses.

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Expression of HLA-DP0401 Molecules for Identification of DP0401 Restricted Antigen Specific T Cells

Cited by 13 publications

References 27 publications

Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes

Identification of an immunodominant region of Fel d 1 and characterization of constituent epitopes

Tetramer-guided epitope mapping reveals broad, individualized repertoires of tetanus toxin-specific CD4+ T cells and suggests HLA-based differences in epitope recognition

Five HLA-DP Molecules Frequently Expressed in the Worldwide Human Population Share a Common HLA Supertypic Binding Specificity

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