Expression of HIWI in Human Hepatocellular Carcinoma

Jiang, Jianxia; Zhang, Hongjie; Tang, Qiyun; Bo, Hao; Shi, Ruihua

doi:10.1007/s12013-011-9160-1

Cited by 28 publications

(31 citation statements)

References 21 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When combined, these results indicate presence of the piRNA pathway in human liver and its modulation during neoplastic transformation. This result is in line with those suggesting the potential of PIWI proteins as cancer diagnostic and prognostic markers [7], including a positive correlation between HIWI expression and liver tumor size and metastasis, matched by a negative one with survival rate [39–41]. Furthermore, MAEL, a key gene in the piRNA biogenesis pathways, has been recognized as oncogene for its role in HCC development and progression [42].…”

Section: Discussionsupporting

confidence: 83%

Specific patterns of PIWI-interacting small noncoding RNA expression in dysplastic liver nodules and hepatocellular carcinoma

et al. 2016

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is the result of a stepwise process, often beginning with development within a cirrhotic liver of premalignant lesions, morphologically characterized by low- (LGDN) and high-grade (HGDN) dysplastic nodules. PIWI-interacting RNAs (piRNAs) are small noncoding RNAs (sncRNAs), 23–35 nucleotide-long, exerting epigenetic and post-transcriptional regulation of gene expression. Recently the PIWI-piRNA pathway, best characterized in germline cells, has been identified also in somatic tissues, including stem and cancer cells, where it influences key cellular processes.Small RNA sequencing was applied to search for liver piRNAs and to profile their expression patterns in cirrhotic nodules (CNs), LGDN, HGDN, early HCC and progressed HCC (pHCC), analyzing 55 samples (14 CN, 9 LGDN, 6 HGDN, 6 eHCC and 20 pHCC) from 17 patients, aiming at identifying possible relationships between these sncRNAs and liver carcinogenesis. We identified a 125 piRNA expression signature that characterize HCC from matched CNs, correlating also to microvascular invasion in HCC. Functional analysis of the predicted RNA targets of deregulated piRNAs indicates that these can target key signaling pathways involved in hepatocarcinogenesis and HCC progression, thereby affecting their activity. Interestingly, 24 piRNAs showed specific expression patterns in dysplastic nodules, respect to cirrhotic liver and/or pHCC.The results demonstrate that the PIWI-piRNA pathway is active in human liver, where it represents a new player in the molecular events that characterize hepatocarcinogenesis, from early stages to pHCC. Furthermore, they suggest that piRNAs might be new disease biomarkers, useful for differential diagnosis of dysplastic and neoplastic liver lesions.

show abstract

Section: Discussionsupporting

confidence: 83%

Specific patterns of PIWI-interacting small noncoding RNA expression in dysplastic liver nodules and hepatocellular carcinoma

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Some studies have demonstrated that HiWi plays a key role in the development of tumors in cervical (19), colon (20), and liver cancer (21). Sun et al (15) also found that HIWI was expressed specifically in glioma tissues and cell lines, but its role and molecular mechanisms related to the development of glioma remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Silencing HIWI suppresses the growth, invasion and migration of glioma cells

Wang

Tong

Gao

et al. 2014

International Journal of Oncology

View full text Add to dashboard Cite

Abstract. the HiWi gene is one of the members of the PiWi gene family that is important for stem cell self-renewal and expressed highly in certain human tumors. Some studies have demonstrated that HiWi plays a key role in the development of tumors in cervical, colon and liver cancer. Previous studies have demonstrated that HiWi is associated with prognosis of patients with glioma. However, there is no report on the analysis of HiWi in the biological characteristics of glioma cells. the aim of the study was to investigate whether HiWi plays an important role in the progress of glioma. Silencing HiWi inhibited cell proliferation by promoting apoptosis and increased cell cycle arrest. the expression of proteins related to apoptosis and the cell cycle, including p21, cyclin D1, Bcl-2, and Bax was significantly altered. Moreover, knockdown of HiWi inhibited the migration and invasion of glioma cells by reducing the expression of MMP-2 and MMP-9. furthermore, we found that reduction of HiWi inhibited tumor growth in vivo. These findings suggest that HIWI is an oncogene involved in the progression of glioma.

show abstract

“…Note HIWI expression in hepatocellular carcinoma tissues was significantly higher than in adjacent normal hepatic tissue and was correlated with metastasis (Jiang et al, 2011). HIWI expression positively correlated with tumor size and metastasis and negatively correlated with survival rates in hepatocellular carcinoma cells and tissues .…”

Section: Liver Cancermentioning

confidence: 85%