Expression of histocompatibility antigens and characterization of the lymphocyte infiltrate in hyperplastic polyps of the large bowel

Bédossa, Pierre; Poynard, Thierry; Bacci, Josette; Naveau, Sylvie; Lemaigre, G; Chaput, J C; Martin, E

doi:10.1016/0046-8177(90)90233-u

Cited by 11 publications

(9 citation statements)

References 18 publications

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“…This observation is consistent with previous studies which showed that the proliferative zone in normal colonic epithelium is located in the lower part of the crypts; the methods used included tritiated thymidine incorporation,27 30 tritiated thymidine, uridine, and leucine in combination,3' bromodeoxyundine uptake,' Ki67 immunoreactivity,32 33 and PCNA immunoreactivity. '7 34 These studies generally found no evidence of proliferation in the upper third of the crypt or in the surface epithelium of normal intestine.…”

Section: Discussionsupporting

confidence: 91%

“…This concept is supported by studies of the adenoma-carcinoma sequence which show a shift in the site of the proliferating cell population towards the surface. 128 32 33 35 36 43 In some adenomas evidence of proliferation may be confined entirely to the luminal third of the crypt,' 2 28 43 44 although this feature was not observed in this study. A previous study of PCNA immunoreactivity in large intestinal tubular adenomas reported that, although PCNA immunoreactive cells were found throughout the lesions, most immunoreactivity was seen in the crypt bases.34 This study also demonstrated a correlation between overexpression of p53 immunoreactivity and the PCNA labelling index.…”

Section: Discussionmentioning

confidence: 66%

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Expression of proliferating cell nuclear antigen in hyperplastic polyps, adenomas and inflammatory cloacogenic polyps of the large intestine.

Carr

Monihan²,

Nzeako³

et al. 1995

Journal of Clinical Pathology

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Aims-To compare proliferating cell nuclear antigen (PCNA) immunoexpression in hyperplastic polyps, adenomas, and inflammatory cloacogenic polyps of the human colon and rectum using paraffin wax embedded tissue. Methods-The monoclonal antibody PC10 was used to demonstrate PCNA immunoreactivity in 88 polypoid lesions from 68 patients. Cases in which immunoexpression was completely absent were excluded, leaving 32 hyperplastic polyps, 31 adenomas, and seven inflammatory cloacogenic polyps for analysis. Labelling indices for the upper and lower third of each lesion and for adjacent normal mucosa were calculated. Results-The upper third labelling indices for adenomas were substantially higher than those for hyperplastic polyps or normal mucosa, whereas those for the upper thirds of hyperplastic polyps and normal mucosa did not differ greatly. The differences between the lower third samples were not significant. In 16 (50%) hyperplastic polyps positive cells persisted onto the luminal surface. Some adenomas showed the most intense staining and the highest labelling indices in the upper third, with strong staining of surface cells; this pattern was not seen in the other lesions. The inflammatory cloacogenic polyps did not show a consistent pattern of immunoexpression. Conclusions-Differences in cell kinetics between adenomas, hyperplastic polyps, and normal mucosa may be shown in formalin fixed, paraffin wax embedded tissue using PC10 as a marker of proliferative activity. PCNA expression also persists into the upper portions of hyperplastic polyps. Assuming that hyperplastic polyps are hypermature lesions with a slower rate of cell migration, this finding suggests that there may be an alteration in PCNA protein metabolism. (7 Clin Pathol 1995;48:46-52)

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 66%

Expression of proliferating cell nuclear antigen in hyperplastic polyps, adenomas and inflammatory cloacogenic polyps of the large intestine.

Carr

Monihan²,

Nzeako³

et al. 1995

Journal of Clinical Pathology

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“…In agreement with the present study, Bedossa et al (1990) found no expression of HLA-DR in the epithelium of 15 hyperplastic polyps, in contrast to 8 of 15 adenomas, which showed patchy positivity. Jen et al (1994), however, found K-ras mutations in 5 of 22 hyperplastic polyps with diameter less than 10 mm.…”

Section: Discussionsupporting

confidence: 81%

“…Although some groups claim to find expression of HLA-DR in normal colorectal epithelium in humans (McDougall et al, 1990;Tsioulias et al, 1992;Mayer and Shlien, 1987), most investigations do not (Daar et al, 1982(Daar et al, , 1984Ghosh et al, 1986;Momburg et al, 1986;Gutierrez et al, 1987;Degener et al, 1988;Ruiz-Cabello et al, 1988;Norazmi et al, 1989;Bedossa et al, 1990;Horie et al, 1990). The discrepancy may be explained by the selection of normal controls, as slight inflammatory changes are known to induce DR-expression (Rognum et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

K-ras mutations and HLA-DR expression in large bowel adenomas

Andersen

Breivik²,

Løvig³

et al. 1996

Br J Cancer

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Summary A total of 72 sporadic colorectal adenomas in 56 patients were studied for the presence of point mutations in codons 12 and 13 of the K-ras gene and for HLA-DR antigen expression related to clinicopathological variables. Forty K-ras mutations in 39 adenomas were found (54%): 31 (77%) in codon 12 and nine (23%) in codon 13. There was a strong relationship between the incidence of K-ras mutations and adenoma type, degree of dysplasia and sex. The highest frequency of K-ras mutations was seen in large adenomas of the villous type with high-grade dysplasia. Fourteen out of 15 adenomas obtained from 14 women above 65 years of age carried mutations. HLA-DR positivity was found in 38% of the adenomas, large tumours and those with high-grade dysplasia having the strongest staining. Coexpression of K-ras mutations and HLA-DR was found significantly more frequently in large and highly dysplastic adenomas, although twoway analysis of variance showing size and grade of dysplasia to be the most important variable. None of the adenomas with low-grade dysplasia showed both K-ras mutation and HLA-DR positivity (P=0.004). K-ras mutation is recognised as an early event in colorectal carcinogenesis. The mutation might give rise to peptides that may be presented on the tumour cell surface by class II molecules, and thereby induce immune responses against neoplastic cells.

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“…cells (Daar et al, 1984;Natali et al, 1984). It has been established also that HLA class I1 antigen can be expressed in certain circumstances in many epithelia that are negative in basal conditions (Bedossa et al, 1990). Recently, it has been demonstrated that there are two different types of tissue-specific genetic control for class I1 antigens: (1) in cells like B lymphocytes, with constitutive expression due to constant stimulation by transacting mechanisms, and (2) in cells in which the expression of class I1 molecules only is inducible, as in macrophages.…”

mentioning

confidence: 99%

Different Patterns of Hla‐dr Antigen Expression in Normal Epithelium, Hyperplastic and Neoplastic Malignant Lesions of the Breast

Concha¹,

Ruiz‐Cabello²,

Cabrera³

et al. 1995

Int J Immunogenetics

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Fifteen samples of non-tumoural breast tissue, 24 cases of benign lesions, four biopsies of inflammatory carcinomas and 94 tumour samples of primitive mammary carcinomas were analysed for HLA class II expression. We found, first, that HLA class II antigens were detectable in all cases of non-neoplastic breast tissue. Secondly, HLA class II antigen expression was notably increased in benign neoplasms and hyperplastic lesions. In contrast, only 32 out of 94 carcinomas showed expression of HLA-DR antigens, 17 tumours had HLA-DP antigens and 11 carcinomas were positive for the presence of DQ molecules. The expression of class II antigen was associated with the degree of histological differentiation (P < 0.05) but was independent of stromal leucocytic infiltration. Thirdly, HLA-DR was very strongly expressed in intravascular tumoural thrombi, especially in the 'inflammatory carcinomas'. The immunophenotype of inflammatory infiltrate was analysed in benign and malignant lesions. In malignant lesions the mean number of inflammatory cells was significantly higher than in benign lesions. Interestingly, we found no differences in the amount and composition of inflammatory infiltrate between HLA-DR positive and negative tumours.

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Expression of histocompatibility antigens and characterization of the lymphocyte infiltrate in hyperplastic polyps of the large bowel

Cited by 11 publications

References 18 publications

Expression of proliferating cell nuclear antigen in hyperplastic polyps, adenomas and inflammatory cloacogenic polyps of the large intestine.

Expression of proliferating cell nuclear antigen in hyperplastic polyps, adenomas and inflammatory cloacogenic polyps of the large intestine.

K-ras mutations and HLA-DR expression in large bowel adenomas

Different Patterns of Hla‐dr Antigen Expression in Normal Epithelium, Hyperplastic and Neoplastic Malignant Lesions of the Breast

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