Expression of Highly Active Bacterial Phospholipase A2 in Yeast Using Intein-Mediated Delayed Protein Autoactivation

Cheperegin, S. E.; Malysheva, A. V.; Sannikova, E. P.; Gubaidullin, I. I.; Efremov, B. D.; Козлов, Д. Г.

doi:10.1007/s12010-020-03333-7

Cited by 2 publications

(1 citation statement)

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“…Additional biotechnology uses have been found for a mini-intein of only 157 amino acids present in the splicing-factor protein PRP8 of strain P. chrysogenum Pch-1 [ 365 ]. This mini-intein was recently used for expression of a bacterial phospholipase A2 in yeast with a strategy of intein-mediated delayed protein autoactivation to avoid the phospholipase toxic effect on the cell membrane [ 366 ]. P. chrysogenum strains have also been described as fungal endophytes promoting plant growth, as in the case of a P. chrysogenum root endophyte of Antarctic vascular plants, which was proposed by Oses-Pedraza et al [ 367 ] to play a pivotal ecological role in Antarctic terrestrial ecosystems.…”

Section: Epiloguementioning

confidence: 99%

Penicillium chrysogenum, a Vintage Model with a Cutting-Edge Profile in Biotechnology

et al. 2022

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The discovery of penicillin entailed a decisive breakthrough in medicine. No other medical advance has ever had the same impact in the clinical practise. The fungus Penicillium chrysogenum (reclassified as P. rubens) has been used for industrial production of penicillin ever since the forties of the past century; industrial biotechnology developed hand in hand with it, and currently P. chrysogenum is a thoroughly studied model for secondary metabolite production and regulation. In addition to its role as penicillin producer, recent synthetic biology advances have put P. chrysogenum on the path to become a cell factory for the production of metabolites with biotechnological interest. In this review, we tell the history of P. chrysogenum, from the discovery of penicillin and the first isolation of strains with high production capacity to the most recent research advances with the fungus. We will describe how classical strain improvement programs achieved the goal of increasing production and how the development of different molecular tools allowed further improvements. The discovery of the penicillin gene cluster, the origin of the penicillin genes, the regulation of penicillin production, and a compilation of other P. chrysogenum secondary metabolites will also be covered and updated in this work.

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Section: Epiloguementioning

confidence: 99%