Expression of HGF, KGF, EGF and Receptor Messenger RNAs Following Corneal Epithelial Wounding

Wilson, Steven E.; Chen, Lisha; Mohan, Rajiv R.; Liang, Qianwa; Liu, Janice

doi:10.1006/exer.1998.0603

Cited by 163 publications

(115 citation statements)

References 13 publications

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“…It may be that EGF/EGFR levels and activation of EGFR are not responsive to environmental stimuli and poisons in the corneal epithelium. Alternatively, the corneal epithelium may possess homeostatic mechanisms of the kind demonstrated by Wilson et al, 39) or be protected by the corneal blood-epithelium barrier.…”

Section: Discussionmentioning

confidence: 99%

Direct inhibition of EGF receptor activation in vascular endothelial cells by gefitinib (‘Iressa’, ZD1839)

et al. 2004

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Section: Discussionmentioning

confidence: 99%

Direct inhibition of EGF receptor activation in vascular endothelial cells by gefitinib (‘Iressa’, ZD1839)

et al. 2004

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“…TGFb is expressed in corneal tissue. [89][90][91][92][93][94][95][96][97] An epithelial defect in the cornea must be rapidly resurfaced to avoid microbial infection and further damage to the underlying stroma. Healing of an epithelial defect is achieved by migration of epithelial cells, followed by an enhancement of cell proliferation for re-establishment of the epithelium stratification.…”

Section: Roles Of Tgfb Siganling In Corneal Wound Healingmentioning

confidence: 99%

TGFβ pathobiology in the eye

Saika

2006

Laboratory Investigation

239

185

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“…During corneal wound repair, mRNA levels of HGF, KGF and their receptors have been found to be upregulated (Werner et al, 1992;Werner et al, 1994b;Wilson et al, 1999). To assess the possible effect of HGF and KGF on limbal epithelial cell growth in a normal cornea, we probed the expression levels of HGFR and KGFR in the corneal and limbal epithelium by immunohistochemistry.…”

Section: Hgf and Kgf Stimulate The Growth Of Limbal Epithelial Cellsmentioning

confidence: 99%

The growth-promoting effect of KGF on limbal epithelial cells is mediated by upregulation of ΔNp63α through the p38 pathway

Cheng

Wang

Kao

et al. 2009

Journal of Cell Science

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Corneal epithelial stem cells are thought to reside in the limbus, the transition zoon between cornea and conjunctiva. Keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) are two paracrine factors that regulate the proliferation, migration and differentiation of the limbal epithelial cells; however, the underlying mechanisms are still poorly understood. In an ex vivo limbal explant culture, we found that KGF is a more potent growth stimulator for the epithelial outgrowth than HGF. Immunofluorescence studies of the epithelial outgrowth from cells treated with HGF or KGF showed similar expression patterns of keratin-3 and keratin-14. Interestingly, p63 was highly expressed in KGF-treated limbal epithelial sheets but not in those treated with HGF. Kinase inhibitor studies showed that induction of ΔNp63α expression by KGF is mediated via the p38 pathway. The effect of KGF on limbal epithelial outgrowth was significantly reduced when endogenous ΔNp63α was suppressed, suggesting that KGF-induced limbal epithelial outgrowth is dependent on the expression of ΔNp63α. Our findings strongly suggest that limbal keratocytes regulate limbal epithelial cell growth and differentiation through a KGF paracrine loop, with ΔNp63α expression as one of the downstream targets.

show abstract

Expression of HGF, KGF, EGF and Receptor Messenger RNAs Following Corneal Epithelial Wounding

Cited by 163 publications

References 13 publications

Direct inhibition of EGF receptor activation in vascular endothelial cells by gefitinib (‘Iressa’, ZD1839)

Direct inhibition of EGF receptor activation in vascular endothelial cells by gefitinib (‘Iressa’, ZD1839)

TGFβ pathobiology in the eye

The growth-promoting effect of KGF on limbal epithelial cells is mediated by upregulation of ΔNp63α through the p38 pathway

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