Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea

Molecular characterization of heat shock protein 70 genes in the liver of three warm freshwater fishes with differential tolerance to microcystin‐LR

“…This is consistent with previous mammalian studies, in which mice mRNA expression level was found to increase after MC‐LR exposure 42. It has been reported that concentrations of toxic sediments lead to elevated hsp70 levels in fish cell line 43. It is believed that the induction of HSP70 is an evolutionary conserved response of the organisms toward exposure to pro‐oxidants.…”

Up-regulation of hepatic ABCC2, ABCG2, CYP1A1 and GST in multixenobiotic-resistant killifish (Fundulus heteroclitus) from the Sydney Tar Ponds, Nova Scotia, Canada

“…Both heavy metals and PAH can induce HSP70 expression so that heat shock protein expression was considered as an alternative survival strategy to ABCB1 up-regulation in Tar Pond killifish. In the digestive gland of the mussel Mytilus edulis, HSP70 gene expression was elevated after exposure to the heavy metal Hg 2+ (Franzellitti and Fabbri, 2006) and a carp-derived (Cyprinus carpio) skin cell line expressed more HSP70 protein after incubation with different PAHs (Kinder et al, 2006). Based on current results, HSP70 up-regulation is likely not a strategy for pollution tolerance in Tar Pond killifish.…”

“…Gene and protein expression were analyzed in the liver, which is one of the major detoxifying organs of vertebrates. In addition, hepatic heat shock protein 70 (HSP70) expression was evaluated since it can be induced by heavy metals and PAHs (Kinder et al, 2006;Franzellitti and Fabbri, 2006) and could represent an alternative strategy to multixenobiotic-resistance. New tools for quantitative RT-PCR were developed for assessing expression of the genes ABCB1, ABCB11, ABCC2, ABCG2 and GST-mu as part of this study.…”

Persistent Pollution – Past, Present and Future

“…Many heat shock proteins (HSPs) assist the protein folding in their function as molecular chaperones and are upregulated in expression levels by heat or other (e.g., chemical) stress as a response to the denaturation of proteins (Wiegant et al 1994;Kinder et al 2007) if the proportion of un-or misfolded proteins is increased. Many heat shock proteins (HSPs) assist the protein folding in their function as molecular chaperones and are upregulated in expression levels by heat or other (e.g., chemical) stress as a response to the denaturation of proteins (Wiegant et al 1994;Kinder et al 2007) if the proportion of un-or misfolded proteins is increased.…”