Expression of heat shock protein 27 in adult human third molar dental pulp

Abstract

This study is the first to define the expression of hsp 27 in the pulp of the adult human third molar. Using a monoclonal antibody against human hsp 27, immunoreactivity was demonstrated in the odontoblasts, odontoblast processes, pulp fibroblasts, and smooth muscle and endothelial cells of vessel walls. Nerves were negative. Pulp fibroblasts were characterized by cytoplasmic staining and variable nuclear staining. Odontoblasts also displayed consistent cytoplasmic staining and variable nuclear staining. Western, Northern, and RT-PCR analysis confirmed the expression of hsp 27 mRNA and protein. Hsp 27 was also shown to be present in both the unphosphorylated and phosphorylated isoforms. In general, nuclear localization and phosphorylation of hsp 27 has been correlated with cells responding to stress or other stimuli. This study demonstrates that pulp from a single human third molar provides sufficient material to support a detailed molecular analysis of gene expression.

Immunohistochemical expression of heat shock protein27 in the mouse dental pulp after immediate teeth separation

“…However, in our search for literatures, no study has been conducted so far examining the expression of HSPs during cytotoxic changes in pulp cells. Sens et al [15] reported the expression of HSP in odontoblasts, odontoblast's processes (dentinal fibers), dental pulp fibroblasts, endothelial cells, and vascular smooth muscle cells in human 3rd molars. Further, Tate et al [16] and Suzuki et al [17] verified the expression of HSP in dental pulp cells after experimental cavity preparation by laser ablation.…”

Immunohistochemical localization of heat shock protein 25 (HSP 25) during root formation of the rat molar

“…Rodent incisors grow continuously throughout life, so the difference in tooth type may account for the discrepancy in the immunoreactions. Sens et al (1996) demonstrated the presence of HSP 25 in odontoblasts in the human third molar, and proposed that HSP 25 has a role in the protection and recovery of odontoblasts from daily environmental extremes experienced in the oral cavity. The constant and intense expression of HSP 25 in odontoblasts at the crown portion of the rat molar is similar to that in the rat incisor (Ohshima et al, 2000, 2001), which is consistent with Sens et al's (1996) hypothesis.…”

“…Sens et al (1996) demonstrated the presence of HSP 25 in odontoblasts in the human third molar, and proposed that HSP 25 has a role in the protection and recovery of odontoblasts from daily environmental extremes experienced in the oral cavity. The constant and intense expression of HSP 25 in odontoblasts at the crown portion of the rat molar is similar to that in the rat incisor (Ohshima et al, 2000, 2001), which is consistent with Sens et al's (1996) hypothesis. Thus, HSP 25 has appreciable roles in both the morphological changes of young odontoblasts during odontogenesis, and in the protection and recovery of mature odontoblasts at the crown portion.…”

“…HSP 25 is found in several types of human tissues, including breast, uterus, oviduct, placenta, skin, and platelets (Ciocca et al, 1983, 1993; McClaren and Iseroff, 1994; Arrigo and Préville, 1999). Sens et al (1996) demonstrated in oral tissues that the HSP 25 was localized in odontoblasts, fibroblasts, and endothelial cells of vascular walls of the dental pulp of human third molars. HSP 25 is also present in ameloblasts, cells of enamel‐free areas, odontoblasts, and pulp mesenchymal cells in rat incisors and molars (Ohshima et al, 2000; Otsuka et al, 2001).…”

Pulpal Responses to Caries and Dental Repair

“…The heat shock proteins represent a family of constitutive and inducible proteins, the expression of which may enhance the ability of cells to withstand stress or which may signal up-regulation of reparative responses during cellular injury [Ang et al, 1991]. The expression of heat shock proteins in odontoblasts and various cells of the pulp [Sens et al, 1996[Sens et al, , 1997 indicates that they are possibly involved in resisting injury during cellular stress. It will be interesting to examine heat shock protein expression during caries beneath lesions of varying activity.…”