Expression of heat shock protein (Hsp) 70 and Hsp 40 in gastric cancer

Isomoto, Hajime; Oka, M.; Yano, Yoshitsugu; Kanazawa, Yusei; Soda, Hiroshi; Terada, Ryusuke; Yasutake, Toru; Nakayama, Toshiyuki; Shikuwa, Saburo; Takeshima, Fuminao; Udono, Heiichiro; Murata, Ikuo; Ohtsuka, Kenzo; Kohno, Shigeru

doi:10.1016/s0304-3835(03)00305-7

Cited by 80 publications

(58 citation statements)

References 18 publications

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“…The most prominent HSPs of mammalian cells have molecular weights of approximately 90 000 (HSP90) and 70 000 (HSP70). Many studies have suggested a possible correlation between the expression of HSPs and the growth and differentiation of tumor cells [16][17][18][19][20][21][22] . Recent evidence reveals that a considerable expression of HSPs can be found in non-heat-shocked cells showing that HSPs may be induced by other stresses and participate in normal growth and development of the body [23] .…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have suggested that HSPs play some role during cell growth and differentiation of tumor cells [20,21] . Continuous expression of HSP in tumor cells may be required to serve as molecular chaperones in regulating and stabilizing tumor growth.…”

Section: Discussionmentioning

confidence: 99%

“…It has been verified that HSP70 and grp94 have better molecular chaperones, which can process and present weak viral, bacterial or tumor antigens to host APC, gendering specific T-cell response and CTL reaction [16][17][18][19][20][21] , even anchor antigens on the cell membrane and directly present antigen to NK or T + cells [22][23][24] . Several studies have shown that HSP70-associated peptides could directly activate T + lymphocytes or natural killer cells as superantigens without being dependent on the stimulation of MHC-I class molecules [6][7][8][9][10] .…”

Section: Discussionmentioning

confidence: 99%

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Expression and significance of heat shock protein 70 and glucose-regulated protein 94 in human esophageal carcinoma

Wang

Liu²,

Ai-li³

et al. 2004

WJG

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AIM:To investigate the expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human esophageal carcinoma and adjacent normal tissues. METHODS:The expression of HSP70 and grp94 in 78 human esophageal cancer and adjacent normal tissues was studied by immunohistochemistry and pathology photograph analysis. RESULTS:Both esophageal cancer and adjacent normal tissues could express HSP70 and grp94. Of the 78 cases of esophageal carcinoma, 95.0%(72/78) showed positive HSP70, mainly stained in nuclei, while grp94 was mainly stained in cell plasma, and the positive rate was 71.8% (56/78).There was a significant difference in the expression of HSP70 and grp94 between esophageal cancer and adjacent normal tissues (P<0.01). Compared with adjacent normal tissues, there was a significant difference between differential types and HSP70 expression (P<0.01).CONCLUSION: HSP70 and grp94 express differently in cell plasma and nuclei. The expression intensity of HSP70 is related to the differentiation of esophageal carcinoma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Expression and significance of heat shock protein 70 and glucose-regulated protein 94 in human esophageal carcinoma

Wang

Liu²,

Ai-li³

et al. 2004

WJG

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“…The first indication of Hsp70 involvement in cancer comes from its overexpression in tumors [29][30][31]. Sometimes levels of chaperone transcription factor (HSF1) are directly related to the severity ofcancer [32][33][34].…”

Section: Hsp70mentioning

confidence: 99%

Molecular Chaperones and Co-chaperones as Therapeutic Targets for Cancer

Ahmad¹,

Muzaffar²

2016

J Mol Pharm Org Process Res

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“…They are induced when a cell undergoes various types of environmental stresses like heat, cold and oxygen deprivation [24] . Over-expression of HSP70 has been found in gastric mucosa of rats with gastric ulcer and in patients with gastric carcinoma [25,26] .…”

Section: A B C Dmentioning

confidence: 99%

Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells

Peng²,

Chang³

et al. 2007

WJG

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AIM:To observe the effects of pre-moxibustion on apoptosis and proliferation of gastric mucosal cell in rats with stress-induced ulcer, and to analyze the relationship between those effects and the expression of heat-shock protein 70 (HSP70). METHODS:Sixty healthy Sprague Dawley rats were randomly assigned into four groups, namely group A, B, C and D. The animal model of stress ulcer was established by water immersion and restraint stress. The rats in group A, B, and D served as the restraint, model, and non-acupoint controls, respectively, while those in group C received moxibustion at Zusanli and Liangmen points. Immunohistochemical methodology was used to detect the expression of HSP70, apoptosis index (AI, × 10 -6 /μm 2 ) and proliferation index (PCNA-LI, × 10 -6 /μm 2 ). The mucosal expression of transforming growth factor α (TGF-α) was detected by radioimmunoassay. RESULTS:Moxibustion at Zusanli and Liangmen points significantly decreased the gastric injury and the apoptosis of gastric mucosal cells, while markedly increased the mucosal expression of TGF-α and HSP70 as well as the proliferation of gastric mucosal cells.Compared with group A, ulcer index (UI) (26.8 ± 9.8 vs 12.0 ± 5.9, P < 0.01), AI (9.6 ± 4.2 vs 4.4 ± 2.6, P < 0.05) and expression of HSP70 (9.6 ± 4.2 vs 4.4 ± 2.6, P < 0.05) were significantly increased, but the content of TGF-α (104.7 ± 51.2 pg/mL vs 254.0 ± 86.9 pg/mL, P < 0.01) and PCNA-LI (6.9 ± 4.7 vs 14.9 ± 4.6, P < 0.05) were significantly decreased in group B. However, ulcer index values (UI) and AI were obviously lower in group C compared to groups B and D (14.1 ± 5.4 vs 26.8 ± 9.8 and 26.2 ± 7.7, P < 0.01; 3.0 ± 1.6 vs 9.6 ± 4.2 and 8.2 ± 5.2, P < 0.05, respectively), but content of TGF-α (237.0 ± 72.6 pg/mL vs 104.7 ± 51.2 pg/mL and 154.1 ± 61.3 pg/mL, P < 0.01) and expression of HSP70 (0.13 ± 0.03 vs 0.08 ± 0.06 and 0.06 ± 0.04, P < 0.05) were higher in group C. Furthermore, the PCNA-LI was significantly higher in group C than in group B (21.6 ± 4.1 vs 6.9 ± 4.7, P < 0.01). CONCLUSION:Moxibustion at Zusanli and Liangmen points has a protective effect on rats gastric mucosa in stress-induced gastric ulcer, which is closely related to its actions in promoting synthesis of TGF-α and proliferation of gastric mucosal cells, suppressing gastric mucosal cell apoptosis, and up-regulating HSP70 expression.

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Expression of heat shock protein (Hsp) 70 and Hsp 40 in gastric cancer

Cited by 80 publications

References 18 publications

Expression and significance of heat shock protein 70 and glucose-regulated protein 94 in human esophageal carcinoma

Expression and significance of heat shock protein 70 and glucose-regulated protein 94 in human esophageal carcinoma

Molecular Chaperones and Co-chaperones as Therapeutic Targets for Cancer

Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells

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