Expression of haematopoietic stem cell markers, CD133 and CD34 on human corneal keratocytes

Perrella, Giuseppina; Brusini, Paolo; Spelat, Renza; Hossain, Parwez; Hopkinson, Andy; Dua, Harminder S.

doi:10.1136/bjo.2006.097352

Cited by 36 publications

(31 citation statements)

References 20 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the current minimal criteria for MSC are not ideal for assessing the phenotype of CSSC, as one criterion states that MSC should not express CD34. Nevertheless, our group has showed CD34 is a well-established marker for quiescent keratocytes in vivo [14,15], and CD34 has been linked ABCG2 is a molecular determinant of the side population phenotype that is characteristic of stem cells, and has been used previously to identify CSSC [26,44]. ABCG2 is expressed by a wide variety of stem cells including haematopoietic stem cells [45], embryonic stem cells [46], neural stem cells [47], and perhaps most relevantly limbal epithelial stem cells [48].…”

Section: Discussionmentioning

confidence: 99%

“…Markers traditionally used to identify the keratocyte phenotype include aldehyde dehydrogenase (ALDH), keratocan, CD133 and, as originally identified by our group, CD34 [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…While this may prove more easily translatable to the clinic compared to serum, the addition of recombinant growth factors, both in research and clinically, can be costly. In addition, keratocytes express CD34 in vivo [14,15], a marker commonly associated with haematopoietic stem cells, which might suggest that the use of culture media traditionally used to support cells of haematopoietic origin might be suitable.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

et al. 2015

View full text Add to dashboard Cite

(2015) Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17 (12). pp. 1706 -1722 . ISSN 1477 -2566 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/31287/1/Submission%20Manuscript.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the University of Nottingham End User licence and may be reused according to the conditions of the licence. For more details see: http://eprints.nottingham.ac.uk/end_user_agreement.pdf A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. However, the optimal culture conditions are disputed and few direct media comparisons have been performed. In this report, we evaluated several media types to identify the optimal for inducing an in vitro stem cell phenotype. Discussion: Culture medium can significantly influence CSSC phenotype. SCM produced a cell phenotype closest to that of a pluripotent stem cell, and we considerate to be the most appropriate for development as a clinical grade medium for the production of CSSC phenotypes suitable for cell therapy. Methods

show abstract

Section: Discussionmentioning

confidence: 99%

“…Markers traditionally used to identify the keratocyte phenotype include aldehyde dehydrogenase (ALDH), keratocan, CD133 and, as originally identified by our group, CD34 [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Prominin-1 or CD133, a stem cell marker, was detected n the adult and P10 cornea gene expression patterns and presence of the protein was further confirmed in corneal protein extracts. Two recent studies identified this marker in the corneal stroma and a subset of corneal keratocytes (Perrella et al 2007;Thill et al 2007). While prominin-1 is known as a hematopoietic stem cell marker, its expression at such high level in the cornea suggests that it may be a marker for the subset of keratocytes that are derived from bone marrow progenitor cells.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon

Lee

Schumacher

et al. 2008

Experimental Eye Research

View full text Add to dashboard Cite

The cornea continues to mature after birth to develop a fully functional, refractive and protective barrier tissue. Here we investigated the complex biological events underlying this process by profiling global genome-wide gene expression patterns of the immature postnatal day 10 and seven week-old adult mouse cornea. The lens and tendon were included in the study to increase the specificity of genes identified as up regulated in the corneal samples. Notable similarities in gene expression between the cornea and the tendon were in the mesenchymal extracellular matrix collagen (types I, III, V, VI) and proteoglycan (lumican, decorin and biglycan) genes. Expression similarities in the cornea and lens were limited to certain epithelial genes and the crystallins. Approximately 76 genes were over expressed in the cornea samples that showed basal expression levels in the lens and tendon. Thirty-two of these were novel with no known functions in the cornea. These include genes with a potential role in protection against oxidative stress (Dhcr24, Cdo1, Akr1b7, Prdx6), inflammation (Ltb4dh, Wdr1), ion-transport (Pdzk1ip1, Slc12a2, Slc25a17) and transcription (Zfp36l3, Pdzk1ip1). Direct comparison of the cornea of two ages showed selective up regulation of 50 and 12 genes in the P10 and adult cornea, respectively. Of the up regulated P10 genes several encode extracellular matrix collagens and proteoglycans that are stable components of the adult cornea and their high transcriptional activity at P10 indicate a period of active corneal growth and matrix deposition in the young cornea. Much less is known about the genes selectively over expressed in the adult cornea; some relate to immune response and innervation (Npy), and possibly to electron transport (Cyp24a1, Cyp2f2) and others of yet unknown functions in the cornea (Rgs10, Psmb8, Xlr4)). This study detected expression of genes with known functions in the cornea, providing additional validation of the microarray experiments. Importantly, it identified several novel genes whose functions have not been investigated in the cornea.

show abstract

“…Having enriched NCSCs, these free‐floating spheroids attained CSK phenotype after further treatment with bFGF, TGFβ3 and L A 2 P, a reported CSK differentiation protocol 46. The spheroid cells positively expressed CD34, ALDH3A1 and Col8A2, markers for CSKs,47, 48 and they also had low expression levels of KERA and its synthesizing enzymes (B3GNT7, CHST6). In particular, CD34 immunoreactivity was detected in almost half of cell population inside the treated spheroids.…”

Section: Discussionmentioning

confidence: 99%

Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy

Yam

Teo

Setiawan

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest. We harvested PDL cells from young adult teeth extracted because of non‐functional or orthodontic reason and differentiated them towards CSK phenotype using a two‐step protocol with spheroid formation followed by growth factor and cytokine induction in a stromal environment (human amnion stroma and porcine corneal stroma). Our results showed that the PDL‐differentiated CSK‐like cells expressed CSK markers (CD34, ALDH3A1, keratocan, lumican, CHST6, B3GNT7 and Col8A2) and had minimal expression of genes related to fibrosis and other lineages (vasculogenesis, adipogenesis, myogenesis, epitheliogenesis, neurogenesis and hematogenesis). Introduction of PDL spheroids into the stroma of porcine corneas resulted in extensive migration of cells inside the host stroma after 14‐day organ culture. Their quiescent nature and uniform cell distribution resembled to that of mature CSKs inside the native stroma. Our results demonstrated the potential translation of PDL cells for regenerative corneal cell therapy for corneal opacities.

show abstract

Expression of haematopoietic stem cell markers, CD133 and CD34 on human corneal keratocytes

Cited by 36 publications

References 20 publications

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells

Differential gene expression patterns of the developing and adult mouse cornea compared to the lens and tendon

Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy

Contact Info

Product

Resources

About