Expression of H19 imprinted gene in patients with repeated implantation failure during the window of implantation

Zeng, Hong; Fan, Xiuli; Liu, Nenghui

doi:10.1007/s00404-017-4482-x

Cited by 20 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animal study showed that the expression of a series of lncRNAs differed in pregnancy and non-pregnancy endometrium, which might play a vital role in facilitating embryo implantation 11 . The expression of H19 was down-regulated in the endometrium of RIF 12 . The expression of AK124742 was down-regulated together with PSMD6 in the cumulus cells of nonpregnant group compared with the pregnant group 13 .…”

Section: Introductionmentioning

confidence: 92%

Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity

Feng¹,

Shen²,

Lv³

et al. 2018

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Insufficient endometrial receptivity is a major factor leading to implantation failure (IF), and the traditional way of morphological observation of endometrium cannot determine the condition of receptivity sufficiently. Considering that long-noncoding RNAs (lncRNAs) regulate endometrial receptivity and competing endogenous RNA (ceRNA) mechanism works in plenty of biological processes, ceRNA is likely to function in the pathology of IF. In the present study, we aim to construct an implantation failure related lncRNA-mRNA network (IFLMN), and to identify the key lncRNAs as the candidates for predicting endometrial receptivity. The global background network was constructed based on the presumed lncRNA-miRNA and miRNA-mRNA pairs obtained from lncRNASNP and miRTarBase. Differentially expressed genes (DEGs) of IF were calculated using the data of GSE26787, and then re-annotated as differentially expressed mRNAs (DEMs) and lncRNAs (DELs). IFLMN was constructed by hypergeometric test, including 255 lncRNA-mRNA pairs, 10 lncRNAs, and 212 mRNAs. Topological analysis determined the key lncRNAs with the highest centroid. Functional enrichment analyses were performed by unsupervised clustering, GO classification, KEGG pathway, and co-expression module analyses, achieving six key lncRNAs and their ceRNA sub-networks, which were involved in immunological activity, growth factor binding, vascular proliferation, apoptosis, and steroid biosynthesis in uterus and prepared endometrium for embryo implantation. Sixteen endometrial samples were collected during mid-luteal phase, including 8 recurrent implantation failure (RIF) or recurrent miscarriage (RM) women and 8 controls who conceived successfully. Quantitative real-time PCR was performed to compare the expression of the above six lncRNAs, which validated that the expression of all these lncRNAs was significantly elevated in endometrium of RIF/RM patients. Further studies are needed to investigate the underlying mechanism, and the lncRNAs may be developed into predictive biomarkers for endometrial receptivity.

show abstract

Section: Introductionmentioning

confidence: 92%

Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity

Feng¹,

Shen²,

Lv³

et al. 2018

Int. J. Biol. Sci.

View full text Add to dashboard Cite

show abstract

“…During the implantation window, the regulation of mRNA levels by lncRNA-miRNA molecules in the endometrium is critical. This is the case for H19 /let-7a-5p/ ITGB3 where H19 acts as a sponge for let-7a-5b to prevent the degradation of ITGB3 transcript that is translated into a protein necessary for implantation [ 174 , 177 ]. This association between lncRNAs with other RNAs to create ceRNA networks could be involved in many functions needed to prepare the endometrium for embryo implantation, such as immune system activity, angiogenesis, apoptosis, and steroid biosynthesis [ 178 ].…”

Section: Non-coding Rnasmentioning

confidence: 99%

“…In addition, the expression of H19 is downregulated in the secretory endometrium of patients with repetitive implantation failure (RIF) compared to the control group. This differential expression could lead to the downregulation of integrin β-3 necessary for trophoblast adhesion [ 174 , 177 , 186 ]. Furthermore, the differential expression of lncRNAs in patients with RIF has been associated with a dysregulation of pathways necessary for a proper implantation process, such as cell adhesion, tumor necrosis factor signaling, Toll-like receptor signaling, and NK-κB signaling [ 187 , 188 ].…”

Section: Non-coding Rnasmentioning

confidence: 99%

The role of epigenetic mechanisms in the regulation of gene expression in the cyclical endometrium

et al. 2021

View full text Add to dashboard Cite

Background The human endometrium is a highly dynamic tissue whose function is mainly regulated by the ovarian steroid hormones estradiol and progesterone. The serum levels of these and other hormones are associated with three specific phases that compose the endometrial cycle: menstrual, proliferative, and secretory. Throughout this cycle, the endometrium exhibits different transcriptional networks according to the genes expressed in each phase. Epigenetic mechanisms are crucial in the fine-tuning of gene expression to generate such transcriptional networks. The present review aims to provide an overview of current research focused on the epigenetic mechanisms that regulate gene expression in the cyclical endometrium and discuss the technical and clinical perspectives regarding this topic. Main body The main epigenetic mechanisms reported are DNA methylation, histone post-translational modifications, and non-coding RNAs. These epigenetic mechanisms induce the expression of genes associated with transcriptional regulation, endometrial epithelial growth, angiogenesis, and stromal cell proliferation during the proliferative phase. During the secretory phase, epigenetic mechanisms promote the expression of genes associated with hormone response, insulin signaling, decidualization, and embryo implantation. Furthermore, the global content of specific epigenetic modifications and the gene expression of non-coding RNAs and epigenetic modifiers vary according to the menstrual cycle phase. In vitro and cell type-specific studies have demonstrated that epithelial and stromal cells undergo particular epigenetic changes that modulate their transcriptional networks to accomplish their function during decidualization and implantation. Conclusion and perspectives Epigenetic mechanisms are emerging as key players in regulating transcriptional networks associated with key processes and functions of the cyclical endometrium. Further studies using next-generation sequencing and single-cell technology are warranted to explore the role of other epigenetic mechanisms in each cell type that composes the endometrium throughout the menstrual cycle. The application of this knowledge will definitively provide essential information to understand the pathological mechanisms of endometrial diseases, such as endometriosis and endometrial cancer, and to identify potential therapeutic targets and improve women’s health.

show abstract

“…Using high-throughput sequencing analysis, recent studies have attempted to uncover key lncRNAs as biomarkers to predict endometrial receptivity (Koot et al, 2016;Fan et al, 2017;Sigurgeirsson et al, 2017;Chen et al, 2018Chen et al, , 2019Feng et al, 2018), while little is known about their biological function and significance in the human endometrium. The expression of H19 was down-regulated in the endometrium of RIF (Zeng et al, 2017). LncRNA882 acted as a ceRNA for miR-15b and then indirectly regulated the level of LIF in goat endometrial epithelial cells (EECs) (Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A Novel Molecule in Human Cyclic Endometrium: LncRNA TUNAR Is Involved in Embryo Implantation

Wang

Yao

et al. 2020

Front. Physiol.

View full text Add to dashboard Cite

Embryo implantation rate remains an inefficient process in in vitro fertilization and embryo transfer (IVF-ET) cycles. The role long non-coding RNA (lncRNA) plays in embryo implantation remains unclear. We aimed to investigate the expression pattern of lncRNA TCL1 upstream neural differentiation-associated RNA (TUNAR) in human cyclic endometrium and clarify the role of TUNAR in the development of endometrial receptivity. Endometrial biopsies were collected at the late proliferative phase, luteinizing hormone (LH) + 2 and LH + 7, from patients with or without recurrent implantation failure (RIF). Real-time RT PCR was performed to detect the level of lncRNAs. After pZW1-snoVector-TUNAR transfection, multiple function of TUNAR in endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs) was investigated. The expression of TUNAR in endometrium was found down-regulated at LH + 7 and up-regulated in RIF patients. In proliferative phase, TUNAR was overwhelmingly more abundant in ESCs and regulated its proliferation. In LH + 7, the difference in the expression of TUNAR between ESCs and EECs was narrowed. Overexpression of TUNAR not only impaired spheroid attachment to EECs, but also inhibited decidualization of ESCs. TUNAR was found expressed in human endometrium for the first time, which might be involved in embryo implantation by modulating the blastocyst attachment to the endometrial epithelium and regulating the proliferation and decidualization of ESCs. Our study helps us to better understand the molecular mechanisms of embryo implantation and may provide a promising biomarker of endometrial receptivity.

show abstract

Expression of H19 imprinted gene in patients with repeated implantation failure during the window of implantation

Abstract: The expression of H19 lncRNA and integrin β3 protein were down-regulated in the RIF patients.

Cited by 20 publications

References 23 publications

Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity

Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity

The role of epigenetic mechanisms in the regulation of gene expression in the cyclical endometrium

A Novel Molecule in Human Cyclic Endometrium: LncRNA TUNAR Is Involved in Embryo Implantation

Contact Info

Product

Resources

About