Expression of H-Y Antigen in Human Males with Two Y Chromosomes

Abstract

To determine whether the gene that controls the expression of H-Y ("male") antigen on human cells is Y-linked, we have compared the H-Y antigen level in normal males with that in three males with two Y chromosomes. Leukocytes from one XXYY and two XYY males express more H-Y antigen than leukocytes from normal XY males. We conclude that a structural gene or positive regulatory gene for H-Y antigen is on the human Y chromosome. Testing for the H-Y antigen may be of benefit in patients who have signs of masculinization but who lack an identifiable Y chromosome. Positive results for the H-Y antigen would be tentative evidence that the corresponding region of the Y chromosome was present, perhaps as part of a translocation, despite the absence of a typical Y chromosome.

H-Y Antigen: Behavior and Function

“…In the standard cytotoxicity test, mouse sperm were incubated for 50 minutes with both H-Y antiserum and rabbit complement. Trypan-blue dye was added during the last 10 minutes of incubation to stain dead sperm (44). the mouse, she screened all available mouse antiserums to find out what known antigens might be expressed on sperm, including, in her survey, serums from B6 female mice that had rejected grafts from B6 males.…”

“…Positive absorption wood lemmlings are H-Y- (38). 1 (i) in the number of 6) In cattle, chorionic vascular anastoth trypan-blue dye) in d (ii) in the frequency mosis between male and female twin fee MHA-HA test (44). tuses leads to intersexual development in the female twin.…”

Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants.

“…Until recently, there existed no genetic markers for the Y chromosome and thus no way to correlate chromosome structure with male function. This situation is now changing with the discovery of two distinctive cytological characteristics on the human Y chromosome (3,4) and the recognition that a locus for a male histocompatibility antigen is Y-linked (5,6).…”

H‐Y typing by ELISA in a 46,X,dic(Y)(q11.2101) male: Effects of a nonmosaic Yp duplication

“…Duplication of the short arm, as in the subject of the present study, is associated with increased expression of H-Y relative to that in normal males. Wachtel et al, 1975Fraccaro et al, 1982Casanova-Bettane and Fellous, 1981Fraccaro et at. 1982 a brief letter, Teyssier et a1 [1982] described "reduced" expression of H-Y in a man with 48,XYYY.…”