Expression of GUT1, which encodes glycerol kinase in Saccharomyces cerevisiae, is controlled by the positive regulators Adr1p, Ino2p and Ino4p and the negative regulator Opi1p in a carbon source-dependent fashion

Grauslund, Morten; Lopes, John M.; Rønnow, Birgitte

doi:10.1093/nar/27.22.4391

Cited by 78 publications

(59 citation statements)

References 53 publications

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“…Several genes involved in fatty acid and sterol biosynthesis as well as inositol transport have been reported as containing putative UAS INO elements in their promoters (Greenberg and Lopes, 1996). Functional analyses of many of these genes confirm a role for Ino2p and/or UAS INO in their activation (Chirala et al, 1994;Koipally et al, 1996;Grauslund et al, 1999). Our microarray analysis of p180-expressing strains revealed several upregulated genes whose promoters contain known of putative UAS INO elements including INO1, GUT1, and SCS3 (unpublished results).…”

Section: Discussionsupporting

confidence: 52%

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

Block-Alper

Webster

Zhou

et al. 2002

MBoC

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Expression of the 180-kDa canine ribosome receptor inSaccharomyces cerevisiae leads to the accumulation of ER-like membranes. Gene expression patterns in strains expressing various forms of p180, each of which gives rise to unique membrane morphologies, were surveyed by microarray analysis. Several genes whose products regulate phospholipid biosynthesis were determined by Northern blotting to be differentially expressed in all strains that undergo membrane proliferation. Of these, the INO2 gene product was found to be essential for formation of p180-inducible membranes. Expression of p180 in ino2Δ cells failed to give rise to the p180-induced membrane proliferation seen in wild-type cells, whereas p180 expression in ino4Δ cells gave rise to membranes indistinguishable from wild type. Thus, Ino2p is required for the formation of p180-induced membranes and, in this case, appears to be functional in the absence of its putative binding partner, Ino4p.

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Section: Discussionsupporting

confidence: 52%

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

Block-Alper

Webster

Zhou

et al. 2002

MBoC

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“…This novel regulatory route is postulated to proceed through the binding of Adr1p to the PIP2 promoter at a deviant UAS1. UAS1 PIP2 is only the second example, after UAS1 GUT1 (38), of a tandem array of UAS1 half-sites capable of binding Adr1p in vitro. Consistent with Adr1p binding to the PIP2 promoter, the respective levels of PIP2 transcript, gene product, and in vitro binding potential to OREs were found to be reduced in the adr1⌬ mutant compared with the situation in wild-type cells grown under oleic acid medium conditions.…”

Section: Discussionmentioning

confidence: 99%

“…1A). The nucleotide sequence of UAS1 PIP2 resembles that in the promoter of the GUT1 gene (37), which similarly consists of two single Adr1p-binding half-sites ordered as a tandem repeat (38). To examine whether UAS1 PIP2 could interact with Adr1p, EMSAs were performed on a fragment representing this promoter element as well as on the analogous sequence in the CTA1 promoter, UAS1 CTA1 .…”

Section: Uas1 Pip2 Binds Adr1p In Vitro-mentioning

confidence: 99%

Saccharomyces cerevisiae PIP2 Mediating Oleic Acid Induction and Peroxisome Proliferation Is Regulated by Adr1p and Pip2p-Oaf1p

Rottensteiner

Wabnegger

Erdmann

et al. 2003

Journal of Biological Chemistry

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Saccharomyces cerevisiae genes involved in fatty acid degradation contain in their promoters oleate response elements (OREs) and type 1 upstream activation sequences (UAS1s) that bind Pip2p-Oaf1p and Adr1p, respectively. The promoter of the PIP2 gene was found to contain a potential UAS1 that consists of a tandem array of CYCCRR half-sites in an overlapping arrangement with a previously characterized ORE. Electrophoretic mobility shift analysis demonstrated that Adr1p bound to UAS1 PIP2 , and Northern analysis in combination with a lacZ reporter gene confirmed that Adr1p influenced the transcription of PIP2. Immunoprecipitation showed that, in adr1⌬ mutant cells grown on oleic acid, Pip2p was less abundant compared with the corresponding wild-type. In addition, the amount of Pip2p-Oaf1p that bound to a target ORE in vitro was reduced in mutant extracts compared with the wild-type. Transcription of the oleic acid-inducible genes SPS19 and CTA1, which rely on both Pip2p-Oaf1p and Adr1p for their regulation, was reduced in adr1⌬ mutant cells. However, by ectopically restoring levels of Pip2p in adr1⌬ cells grown on oleic acid medium, transcription of both genes increased 2-fold compared with the control. This partial suppression of the adr1⌬ mutant phenotype was additionally manifested by moderate utilization of oleic acid. Hence, both the expression as well as the action of the two transcription factors, Adr1p and Pip2p-Oaf1p, are interconnected, which allows for an elaborate control of fatty acid-inducible genes.

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“…Both genes are regulated by INO2 and INO4, genes encoding transcription factors that are responsive to inositol signaling (56). Growth on glycerol is ADR1-dependent (6), presumably because of the dependence of GUT1 and GUT2 expression on ADR1 (5,6). In the DNA array experiments (Fig.…”

Section: Figmentioning

confidence: 99%

“…The genes of ␤-oxidation that are ADR1-dependent are also regulated by oleate induction through the transcription factor Oaf1⅐Pip2 (8,9). GUT1 is regulated by Ino2 and Ino4 as well as by Adr1 (5). Thus, Adr1 acts in concert with at least three other transcription factors.…”

mentioning

confidence: 99%

Multiple Pathways Are Co-regulated by the Protein Kinase Snf1 and the Transcription Factors Adr1 and Cat8

Young¹,

Dombek²,

Tachibana

et al. 2003

Journal of Biological Chemistry

250

310

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ADR1 and CAT8 encode carbon source-responsive transcriptional regulators that cooperatively control expression of genes involved in ethanol utilization. These transcription factors are active only after the diauxic transition, when glucose is depleted and energy-generating metabolism has shifted to the aerobic oxidation of non-fermentable carbon sources. The Snf1 protein kinase complex is required for activation of their downstream target genes described previously. Using DNA microarrays, we determined the extent to which these three factors collaborate in regulating the expression of the yeast genome after glucose depletion. The expression of 108 genes is significantly decreased in the absence of ADR1. The importance of ADR1 during the diauxic transition is illustrated by the observation that expression of almost one-half of the 40 most highly glucose-repressed genes is ADR1-dependent. ADR1-dependent genes fall into a variety of functional classes with carbon metabolism containing the largest number of members. Most of the genes in this class are involved in the oxidation of different non-fermentable carbon sources. These microarray data show that ADR1 coordinates the biochemical pathways that generate acetylCoA and NADH from non-fermentable substrates. Only a small number of ADR1-dependent genes are also CAT8-dependent. However, nearly one-half of the ADR1-dependent genes are also dependent on the Snf1 protein kinase for derepression. Many more genes are SNF1-dependent than are either ADR1-or CAT8-dependent suggesting that SNF1 plays a broader role in gene expression than either ADR1 or CAT8. The largest class of SNF1-dependent genes encodes regulatory proteins that could extend SNF1 dependence to additional pathways.During the diauxic shift, when yeast cells deplete the glucose in the medium, the flow of metabolites changes dramatically to adapt to the use of alternative energy and carbon sources, primarily ethanol produced during fermentation. The changes in gene expression that are required for this metabolic reorganization were dramatically revealed by DNA microarray analysis (1). The expression of more than one-quarter of the yeast genome is altered when glucose is exhausted. These alterations allow the cell to utilize carbon sources other than glucose to channel metabolites into the tricarboxylic acid and glyoxylate cycles to generate energy and synthesize intermediates for gluconeogenesis. In addition, these changes allow the cell to alter its growth rate and prepare for growth arrest and stationary phase.Numerous transcription factors and regulatory proteins are responsible for the altered transcriptional program that accompanies the depletion of glucose. Two transcription factors that play important roles during the diauxic transition are Adr1 1 and Cat8.

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Expression of GUT1, which encodes glycerol kinase in Saccharomyces cerevisiae, is controlled by the positive regulators Adr1p, Ino2p and Ino4p and the negative regulator Opi1p in a carbon source-dependent fashion

Cited by 78 publications

References 53 publications

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

Saccharomyces cerevisiae PIP2 Mediating Oleic Acid Induction and Peroxisome Proliferation Is Regulated by Adr1p and Pip2p-Oaf1p

Multiple Pathways Are Co-regulated by the Protein Kinase Snf1 and the Transcription Factors Adr1 and Cat8

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