Expression of grapevine chitinase genes in berries and leaves infected by fungal or bacterial pathogens

Robert, Nadia; Roche, Karine; Lebeau, Yann; Breda, C.; Boulay, M.; Esnault, R.; Buffard, Dominique

doi:10.1016/s0168-9452(01)00576-3

Cited by 87 publications

(65 citation statements)

References 35 publications

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“…Using elicitors to strengthen the endogenous defenses of plants constitutes an attractive alternative to current chemical methods. To date, most of the data about defense responses analyzed in V. vinifera during interaction with pathogenic fungi are related to gene expression and activity of chitinases and b-1,3-glucanases (Busam et al 1997;Robinson et al 1997;Robert et al 2002) and the production of stilbene phytoalexins (Adrian et al 1997;Bais et al 2000). Recent studies have shown that a proteinaceous elicitor purified from B. cinerea ) and laminarin derived from the brown alga Laminaria digitata (Aziz et al 2003) induce other defense responses in grapevine, including oxidative burst, calcium influx and activation of two MAP kinases of 45 and 49 kDa.…”

Section: Introductionmentioning

confidence: 99%

Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea

Aziz

Heyraud

Lambert

2004

Planta

176

133

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Grapevine (Vitis vinifera L.) is vulnerable to a variety of pathogenic fungi, among them Botrytis cinerea, the causal agent of grey mould, is responsible for worldwide yield losses that would be even more important without a successful control that relies mainly on fungicides. In the present work we investigated an alternative way of using oligogalacturonides (OGA) to induce defense responses in grapevine and protection against B. cinerea. Kinetic experiments with grapevine cells showed that OGA induced a rapid and transient generation of H 2 O 2 , followed by differential expression of nine defense-related genes and stimulation of chitinase and b-1,3-glucanase activities. Inhibition of OGAinduced oxidative burst by diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, lowered induction levels of six genes and chitinase activity. Interestingly, the induction of three other genes and b-1,3-glucanase activity were inhibited by K252a, a protein kinase inhibitor, but not by DPI. Treatment of grapevine leaves with OGA also reduced infection by B. cinerea by about 55-65%. Accordingly, DPI or K252a with or without OGA increased the susceptibility of grapevine leaves to B. cinerea. We suggest that treatment of grapevine with OGA elicits different signalling pathways, which might act in tandem with the oxidative burst to increase grapevine defense responses required for protection against B. cinerea.

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Section: Introductionmentioning

confidence: 99%

Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea

Aziz

Heyraud

Lambert

2004

Planta

176

133

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“…Differential expression of chitinase genes after inoculation of grape leaves with Botrytis cinerea and Pseudomonas syringae pv. pisi has been reported (Robert et al 2002). Thomma et al (2001) reported that the activation pattern of synthesis of defense-related compounds was different dependently on the type of pathogens in Arabidopsis.…”

Section: Discussionmentioning

confidence: 97%

“…It was reported that the expression of defense-related genes was induced by inoculation with various pathogens in grapevines (Bézier et al 2002;Robert et al 2002;Kortekamp 2006). Differential expression of chitinase genes after inoculation of grape leaves with Botrytis cinerea and Pseudomonas syringae pv.…”

Section: Discussionmentioning

confidence: 99%

Glutathione S-Transferase Genes Differently Expressed by pathogen-Infection in Vitis flexuosa

Ahn¹,

Kim²,

Yun³

2016

Plant Breed. Biotech

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2016 (February) ABSTRACT Glutathione S-transferase (GST) genes from transcripts of Vitis flexuosa leaves infected with Elsinoe ampelina were characterized and analyzed for their expression using primers based on specific regions. Comparison of deduced amino acid sequences from GST transcripts of V. flexuosa showed that the score of the deduced amino acid identity ranged from 43.38% (Vf GST26625 and Vf GST774) to 6.67% (Vf GST13892 and Vf GST774). Primary and secondary structure analysis was performed using the ProtParam and Self-Optimized Prediction Method with Alignment software. A phylogenetic tree was constructed from the GST proteins by the neighbor joining method using MEGA 6.0 to investigate the relationship among Vf GST, VvGST, and At GST proteins. To evaluate the differential expression pattern of GST genes by real-time polymerase chain reaction (PCR), primers specific to unique regions in each gene were obtained by alignment of the sequences. Real-time PCR revealed that GST genes were expressed differentially in the leaves of V. flexuosa infected with Botrytis cinerea, E. ampelina, and Rhizobium vitis. The expression of Vf GST26625 was up-regulated, while that of others were down-regulated among five GSTs in all grapevine leaves inoculated with each pathogen. The results provided herein improve our understanding of defense responses to various pathogen attacks in grapevines.

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“…Indeed, grapevine chitinases are known to be inducible by various biotic stresses but differentially, according to the applied stress, the studied organ, and the grapevine cultivar (Busam et al 1997;Jacobs et al 1999;Robert et al 2002;Aziz et al 2003). PGIP and PIN mRNA transcripts accumulated in treated leaves.…”

Section: Botrytis Cinerea Extract Induces Defense Responses In Grapevinementioning

confidence: 99%

“…1b). CHIT3 is inducible by pathogens such as P. viticola and E. necator or by chemicals such as salicylic acid (SA), 2,6-dichloroisonicotinic acid (INA), or benzothiadiazole (BTH) (Busam et al 1997;Jacobs et al 1999;Robert et al 2001Robert et al , 2002.…”

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confidence: 99%

Oligosaccharides from Botrytis cinerea and Elicitation of Grapevine Defense

et al. 2014

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An extract from Botrytis cinerea culture filtrate was sprayed on grapevine plants (Vitis vinifera) to investigate its potential to stimulate defense reactions. The extract triggered the induction of genes encoding pathogenesis-related (PR) proteins as chitinases (CHIT), polygalacturonase-inhibiting protein (PGIP), serine proteinase inhibitor (PIN), and enzymes involved in phytoalexin synthesis as phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS). Correlated to the up-regulation of these latter genes, stilbene content increased in treated leaves. Consequently, treatment of grapevine leaves with the fungal extract triggered protection toward Plasmopara viticola and Erysiphe necator, the causal agents of grapevine downy and powdery mildews, respectively. Disease severity was significantly reduced in elicited plants, approximately 61 % for downy mildew and 83 % for powdery mildew. This approach could represent a valuable strategy to protect grapevine from diseases as an alternative or complementary method to the use of pesticides.

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Expression of grapevine chitinase genes in berries and leaves infected by fungal or bacterial pathogens

Cited by 87 publications

References 35 publications

Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea

Oligogalacturonide signal transduction, induction of defense-related responses and protection of grapevine against Botrytis cinerea

Glutathione S-Transferase Genes Differently Expressed by pathogen-Infection in Vitis flexuosa

Oligosaccharides from Botrytis cinerea and Elicitation of Grapevine Defense

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