Expression of GPR30, ERα and ERβ in endometrium during window of implantation in patients with polycystic ovary syndrome: a pilot study

Wang, Aiming; Li-juan, JI; Shang, Wei; Li, Min; Chen, Lei; White, Richard E.; Han, Guichun

doi:10.3109/09513590.2010.487584

Cited by 30 publications

(17 citation statements)

References 9 publications

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“…Additionally, in a recent study, the nuclear localization of GPER was demonstrated in fibroblasts, indicating that GPER also mediates a nuclear signaling pathway [23]. Moreover, two recent studies demonstrated nuclear GPER expression in eutopic endometrial cells [24,25]. …”

Section: Discussionmentioning

confidence: 99%

Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a tissue microarray study

Samartzis

Noske

et al. 2012

Reprod Biol Endocrinol

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BackgroundThe G protein-coupled estrogen receptor (GPER) is thought to be involved in non-genomic estrogen responses as well as processes such as cell proliferation and migration. In this study, we analyzed GPER expression patterns from endometriosis samples and normal endometrial tissue samples and compared these expression profiles to those of the classical sex hormone receptors.MethodsA tissue microarray, which included 74 samples from different types of endometriosis (27 ovarian, 19 peritoneal and 28 deep-infiltrating) and 30 samples from normal endometrial tissue, was used to compare the expression levels of the GPER, estrogen receptor (ER)-alpha, ER-beta and progesterone receptor (PR). The immunoreactive score (IRS) was calculated separately for epithelium and stroma as the product of the staining intensity and the percentage of positive cells. The expression levels of the hormonal receptors were dichotomized into low (IRS < 6) and high (IRS > =6) expression groups.ResultsThe mean epithelial IRS (+/−standard deviation, range) of cytoplasmic GPER expression was 1.2 (+/−1.7, 0–4) in normal endometrium and 5.1 (+/−3.5, 0–12) in endometriosis (p < 0.001), of nuclear GPER 6.4 (+/−2.6, 0–12) and 6.8 (+/−2.9, 2–12; p = 0.71), of ER-alpha 10.6 (+/−2.4, 3–12) and 9.8 (+/−3.0, 2–12; p = 0.26), of ER-beta 2.4 (+/−2.2; 0–8) and 5.6 (+/−2.6; 0–10; p < 0.001), and of PR 11.5 (+/−1.7; 3–12) and 8.1 (+/−4.5; 0–12; p < 0.001), respectively. The mean stromal IRS of nuclear GPER expression was 7.7 (+/−3.0; 2–12) in endometrium and 10.8 (+/−1.7; 6–12) in endometriosis (p < 0.001), of ER-alpha 8.7 (+/−3.1; 2–12) and 10.6 (+/−2.4; 2–12; p = 0.001), of ER-beta 1.8 (+/−2.0; 0–8) and 5.4 (+/−2.5; 0–10; p < 0.001), and of PR 11.7 (+/−0.9; 8–12) and 10.9 (+/−2.0; 3–12; p = 0.044), respectively. Cytoplasmic GPER expression was not detectable in the stroma of endometrium and endometriosis. The observed frequency of high epithelial cytoplasmic GPER expression levels was 50% (n = 30/60) in the endometriosis and none (0/30) in the normal endometrium samples (p < 0.001). High epithelial cytoplasmic GPER expression levels were more frequent in endometriomas (14/20, 70%; p = 0.01), as compared to peritoneal (9/18, 50%) or deep-infiltrating endometriotic lesions (7/22, 31.8%). The frequency of high stromal nuclear GPER expression levels was 100% (n = 74/74) in endometriosis and 76.7% (n = 23/30) in normal endometrium (p < 0.001). The frequency of high epithelial nuclear GPER expression levels did not differ between endometriosis and normal endometrium.ConclusionsThe present data indicate a unique GPER expression pattern in endometriosis, especially in endometriomas as compared to the normal endometrium. The overexpression of GPER in endometriotic lesions suggests a potential role for GPER in the hormonal regulation of endometriosis, which should be taken into consideration for future hormonal treatment strategies.

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Section: Discussionmentioning

confidence: 99%

Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a tissue microarray study

Samartzis

Noske

et al. 2012

Reprod Biol Endocrinol

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“…PRB has been shown to physically associate with ERβ in rat uterine stromal cells [38], and it has been shown that treatment with E2 induces PRB expression more than PRA expression in human endometrium [39]. Although increased PR expression [34] and decreased ERβ expression are seen in women with PCOS compared to controls [40], how epithelial cell proliferation is regulated in vivo by the coordinated interaction of stromal PR and ERβ in women with PCOS has yet to be determined.…”

Section: Reviewmentioning

confidence: 99%

Endometrial progesterone resistance and PCOS

et al. 2014

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Polycystic ovary syndrome (PCOS) is a state of altered steroid hormone production and activity. Chronic estrogen exposure or lack of progesterone due to ovarian dysfunction can result in endometrial hyperplasia and carcinoma. A key contributor to our understanding of progesterone as a critical regulator for normal uterine function has been the elucidation of progesterone receptor (PR) expression, regulation, and signaling pathways. Several human studies indicate that PR-mediated signaling pathways in the nucleus are associated with progesterone resistance in women with PCOS. The aim of this review is to provide an overview of endometrial progesterone resistance in women with PCOS; to present the PR structure, its different isoforms, and their expression in the endometrium; to illustrate the possible regulation of PR and PR-mediated signaling in progesterone resistance in women with PCOS; and to discuss current clinical treatments for atypical endometrial hyperplasia and endometrial carcinoma in women with PCOS and accompanying progesterone resistance.

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“…Early abortion accounts for most of the pregnancy failures (Jakubowicz et al, 2002). Abnormal hormonal environments and inflammatory factor expression in vivo can lead to endometrial maldevelopment (Quezada et al, 2006;Villavicencio et al, 2006), which affects embryo implantation (i.e., endometrial receptivity) (Wang, et al, 2011). Even if spontaneous ovulation occurs, the pregnancy rate is low and the early abortion rate is high.…”

Section: Introductionmentioning

confidence: 98%

Expression of apoptosis-related genes in the endometrium of polycystic ovary syndrome patients during the window of implantation

Ling

Wang

Chen

et al. 2012

Gene

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Expression of GPR30, ERα and ERβ in endometrium during window of implantation in patients with polycystic ovary syndrome: a pilot study

Cited by 30 publications

References 9 publications

Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a tissue microarray study

Expression of the G protein-coupled estrogen receptor (GPER) in endometriosis: a tissue microarray study

Endometrial progesterone resistance and PCOS

Expression of apoptosis-related genes in the endometrium of polycystic ovary syndrome patients during the window of implantation

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