Expression of glycogen synthase (GYS) and glycogen synthase kinase 3β (GSK3β) of the Fujian oyster, Crassostrea angulata, in relation to glycogen content in gonad development

Zeng, Zhen; Ni, Jianbin; Ke, Caihuan

doi:10.1016/j.cbpb.2013.09.003

Cited by 33 publications

(37 citation statements)

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“…The glycogen content in various tissues (the mantle, gill, AMs, foot, gonad, and DGs) was examined ( Supplementary Figure S5 ). The glycogen content ranged from 1.69 to 4.4 mg/g wet mass, and was highly detectable in DGs and AMs, and at low levels in the mantle, which is similar to a previous study in Crassostrea angulate 62 . Although muscle is not a glycogen storage tissue in oysters, because its level does not exceed 5% of the total biochemical content 63 , muscle is a repository of active glycogenolysis that quickly provides ATP for muscular contraction in many species 64 .…”

Section: Discussionsupporting

confidence: 89%

PfIRR Interacts with HrIGF-I and Activates the MAP-kinase and PI3-kinase Signaling Pathways to Regulate Glycogen Metabolism in Pinctada fucata

Shi

2016

Sci Rep

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The insulin-induced mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways are major intracellular signaling modules and conserved among eukaryotes that are known to regulate diverse cellular processes. However, they have not been investigated in the mollusk species Pinctada fucata. Here, we demonstrate that insulin-related peptide receptor of P. fucata (pfIRR) interacts with human recombinant insulin-like growth factor I (hrIGF-I), and stimulates the MAPK and PI3K signaling pathways in P. fucata oocytes. We also show that inhibition of pfIRR by the inhibitor PQ401 significantly attenuates the basal and hrIGF-I-induced phosphorylation of MAPK and PI3K/Akt at amino acid residues threonine 308 and serine 473. Furthermore, our experiments show that there is cross-talk between the MAPK and PI3K/Akt pathways, in which MAPK kinase positively regulates the PI3K pathway, and PI3K positively regulates the MAPK cascade. Intramuscular injection of hrIGF-I stimulates the PI3K and MAPK pathways to increase the expression of pfirr, protein phosphatase 1, glucokinase, and the phosphorylation of glycogen synthase, decreases the mRNA expression of glycogen synthase kinase-3 beta, decreases glucose levels in hemocytes, and increases glycogen levels in digestive glands. These results suggest that the MAPK and PI3K pathways in P. fucata transmit the hrIGF-I signal to regulate glycogen metabolism.

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Section: Discussionsupporting

confidence: 89%

PfIRR Interacts with HrIGF-I and Activates the MAP-kinase and PI3-kinase Signaling Pathways to Regulate Glycogen Metabolism in Pinctada fucata

Shi

2016

Sci Rep

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“…When GSI was low, the gonad was not enough for multiple biochemical component determination. Compared to lipid and protein, glycogen is the main energy reserve for gametogenesis in oysters, and the changes in glycogen content during the reproductive cycle reflect the strategy used to provide energy required for gametogenesis (Acarli et al, ; Zeng et al, ). There are two main types of strategy: conservative, in which energy is stored in somatic tissues such as the adductor muscle, digestive glands and mantle; and opportunistic, in which energy is obtained mainly from recently ingested food when food is abundant and of high quality (Bayne, ).…”

Section: Discussionmentioning

confidence: 99%

“…The effect also can be attributed to the variation in glycogen. For gametogenesis, the energy is mainly stored in glycogen (Acarli et al, 2015;Zeng, Ni, & Ke, 2013). Some researchers thought that huge variation in glycogen during reproductive cycle may affect the storage form of metals, thus affecting the metal accumulation (Latouche & Mix, 1981;Páez-Osuna et al, 1995).…”

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confidence: 99%

Influence of gonadal development on metal accumulation in the Portuguese oyster,Crassostrea angulata,in subtropical areas

Shi

Zhou

et al. 2019

Aquac Res

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Metal enrichment makes oyster considered to be an appropriate food source for trace elements, but excessive accumulation can threaten human health. Reproduction is an important biotic factor that affects the metal accumulation in oysters. However, the influence of continuous gonad development model on metal accumulation in some subtropical oysters is still unclear. The present study addresses the concentrations of trace metals and glycogen associated with the gonadal and somatic tissues throughout the reproductive cycle of diploid and triploid Crassostrea angulata cultured in Fujian, China. Cu, Zn and Pb contents of gonads were negatively correlated (p < 0.05) with gonadosomatic index (GSI). The correlation between Cd content and GSI was not significant. There was a positive correlation (p < 0.05) between GSI and Mn content in female gonads while the correlation was negative (p < 0.05) in males, indicating Mn plays an important role in gametogenesis of females. The effects of gonadal maturity and gender on metal accumulation in somatic tissue were not significant (p > 0.05). The concentrations of all metals in sterile triploids were not significantly different from those of the somatic tissue of diploids. Gonadal glycogen content showed a negative correlation with the GSI. However, somatic glycogen content was not affected by gonadal development, indicating that the energy consumed during gametogenesis comes mainly from recently ingested food rather than from energy stored in somatic tissue. The effects of the gonadal development on the metal accumulation in oysters varied according to the metal and tissue type.

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“…In this study, we confirmed that GSK3β increases protein synthesis in triploid oysters and increases myofibrillogenesis via actin polymerization. In oysters such as C. angulata and C. gigas, GSK3β gene expression was reported to be high along with glycogen content in the adductor muscle at the time of gonad development (Zeng et al 2013;Li et al 2017). This stored energy is used for sexual maturity.…”

Section: Discussionmentioning

confidence: 99%

Regulation of adductor muscle growth by the IGF-1/AKT pathway in the triploid Pacific oyster, Crassostrea gigas

Kim

Choi

2019

Fish Aquatic Sci

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We investigated the insulin-like growth factor 1 (IGF-1)/AKT signaling pathway involved in muscle formation, growth, and movement in the adductor muscle of triploid Pacific oyster, Crassostrea gigas. Large and small triploid oysters (LTs and STs) cultured under identical conditions were screened, and the signaling pathways of individuals with superior growth were compared and analyzed. mRNA and protein expression levels of actin, troponin, tropomyosin, and myosin, proteins important in muscle formation, were higher in LTs compared with STs. Expression levels of IGF-1, IGF binding protein (IGFBP), and IGFBP complex acid-labile subunit were also higher in LTs compared with STs. Phosphorylation of the IGF receptor as well as that of AKT was high in LTs. In addition, the expression of phosphomammalian target of rapamycin and phospho-glycogen synthase kinase 3β was increased and the expression of Forkhead box O3 was decreased in LTs. Therefore, we suggested that the IGF-1/AKT signaling pathway affects the formation, growth, and movement of the adductor muscle in triploid oysters.

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Expression of glycogen synthase (GYS) and glycogen synthase kinase 3β (GSK3β) of the Fujian oyster, Crassostrea angulata, in relation to glycogen content in gonad development

Cited by 33 publications

References 50 publications

PfIRR Interacts with HrIGF-I and Activates the MAP-kinase and PI3-kinase Signaling Pathways to Regulate Glycogen Metabolism in Pinctada fucata

PfIRR Interacts with HrIGF-I and Activates the MAP-kinase and PI3-kinase Signaling Pathways to Regulate Glycogen Metabolism in Pinctada fucata

Influence of gonadal development on metal accumulation in the Portuguese oyster,Crassostrea angulata,in subtropical areas

Regulation of adductor muscle growth by the IGF-1/AKT pathway in the triploid Pacific oyster, Crassostrea gigas

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