Expression of glutathione transferase isoenzymes in the porcine ovary in relationship to follicular maturation and luteinization

Eliasson, Maria; Stark, Tuula; DePierre, Joseph W.

doi:10.1016/s0009-2797(98)00096-9

Cited by 9 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, follicular maturation and luteinization increase GSTA1 expression in the bovine and porcine ovary, which also may be induced by pituitary gonadotropins (i.e. follicle-stimulating hormone and luteinizing hormone) (25,26). In addition, 350 and 250 nM estradiol was shown to inhibit human GSTA1 and GSTM1 expression respectively, but not GSTP1 and GSTT1, in the rat liver (27,28).…”

Section: Discussionmentioning

confidence: 99%

Effects of glutathione S-transferase A1 (GSTA1) genotype and potential modifiers on breast cancer risk

Ahn

Gammon²,

Santella

et al. 2006

Carcinogenesis

View full text Add to dashboard Cite

Glutathione S-transferases (GSTs) are phase II enzymes that are involved in the detoxification of a wide range of carcinogens. The novel GSTA1*A and GSTA1*B genetic polymorphism results in differential expression, with lower transcriptional activation of GSTA1*B (variant) than that of GSTA1*A (common) allele. Considering that cruciferous vegetables induce GSTs, which metabolize tobacco smoke carcinogens, we hypothesized that the variant GSTA1*B genotype may predispose women to breast cancer, particularly among low cruciferous vegetable consumers and among smokers. Thus, we evaluated potential relationships between GSTA1 polymorphisms and breast cancer risk, in relation to vegetable consumption and smoking status in the Long Island Breast Cancer Study Project (1996-1997), a population-based case-control study. Genotyping (1036 cases and 1089 controls) was performed, and putative breast cancer risk factors and usual dietary intakes were assessed. Having GSTA1*A/*B or *B/*B genotypes was not associated with increased breast cancer risk, compared to having the common *A/*A genotype. However, among women in the lowest two tertiles of cruciferous vegetable consumption, *B/*B genotypes were associated with increased risk (OR (95% CI)=1.73 (1.10-2.72) for 0-1 servings/week), compared to women with *A/*A genotypes. Among women with *B/*B genotypes, a significant inverse trend between cruciferous vegetable consumption and breast cancer risk was observed (P for trend=0.05), and higher consumption (4+ servings/week) ameliorated the increased risk associated with the genotype. Current smokers with *B/*B genotypes had a 1.89-fold increase in risk (OR (95% CI)=1.89 (1.09-3.25)), compared with never smokers with *A/*A genotypes. These data indicate that GSTA1 genotypes related to reduced GSTA1 expression are associated with increased breast cancer primarily among women with lower consumption of cruciferous vegetables and among current smokers.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of glutathione S-transferase A1 (GSTA1) genotype and potential modifiers on breast cancer risk

Ahn

Gammon²,

Santella

et al. 2006

Carcinogenesis

View full text Add to dashboard Cite

show abstract

“…It was shown that mammalian GSTs have the ability to bind hormones, and particularly sexual 420 steroids, and influence their transport, metabolism, and physiological action (Listowsky et al, 1988). A high 421 binding capacity for steroids was previously displayed by certain isoenzymes of GST (Eliasson et al, 1999;Homma 422 et al, 1986). Particularly, testosterone and progesterone have the ability to bind mammalian GSTs with moderate 423 (10 -6 M < K d < 10 -4 M) or high (K d < 10 -6 M) affinity, respectively (Listowsky et al, 1988).…”

mentioning

confidence: 99%

Molecular characterization of zebrafish Gstr1, the only member of teleost-specific glutathione S- transferase class

et al. 2019

View full text Add to dashboard Cite

First functional characterization of the teleost-specific Gstr1 in zebrafish (Danio rerio);  Recombinant zebrafish Gstr1 protein used for screening inhibition assay;  New insight into structural characteristics of Gstr1 obtained by molecular docking analyses;  Mechanistic interactions of Gstr1 with GSH and endogenous and exogenous compounds revealed;  Proposed role of Gstr1 in steroidogenesis, metabolism and/or physiological actions of androgens;  Organophosphate insecticides and pharmaceuticals identified as potent Gstr1 inhibitors. 1 Molecular characterization of zebrafish Gstr1, the only member of teleost-specific glutathione S-transferase

show abstract

Glutathione and its related enzymes in the gonad of Nile Tilapia (Oreochromis niloticus)

Hamed

Saleh

Shokeer

et al. 2015

Fish Physiol Biochem

View full text Add to dashboard Cite

Glutathione (GSH) concentration, the activity of its metabolizing enzymes, glutathione transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), and the antioxidant enzyme catalase (CAT) in O. niloticus ovary and testis were examined. GSH concentration of O. niloticus testis exhibited high concentration (129 ± 21 nmol/g tissue) compared with GSH concentration (49.2 ± 8.3 nmol/g tissue) in the ovary. GST, GPx, GR, and CAT activities of O. niloticus testis exhibited high values compared with their corresponding values in ovary homogenates. However, protein concentration in ovary homogenates exhibited higher values (175 ± 40.6 mg) compared with testis homogenates (27.1 ± 3.7 mg). O. niloticus ovary was less effective in excretion of xenobiotices compared with the testis, where its function is mainly in increasing the protein content of the eggs; however, in O. niloticus testis, the glutathione cycle operated in accelerated way in the direction of reduced GSH production in order to protect the maturation stages in a save way. A simple reproducible procedure for the purification of GST from O. niloticus ovary was established. The enzymes proved to be homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and its molecular weight was calculated to be 25.1 kDa. GST of O. niloticus ovary exhibited maximum activity at pH 7.5. The Michaelis-Menten constant (K(m)) of the purified ovary GST for GSH and CDNB was 0.076 mM and 1.0 mM, respectively. Cibacron blue was the most potent inhibitor of ovary GST activity (IC50 value, concentration of inhibitor that will give 50% inhibition, equal 0.002 μM). The specific activity of GST toward different electrophilic substrates was determined. GST activity toward benzyl isothiocyanate was the highest compared with phenethyl isothiocyanate and allyl isothiocyanate.

show abstract

Expression of glutathione transferase isoenzymes in the porcine ovary in relationship to follicular maturation and luteinization

Cited by 9 publications

References 22 publications

Effects of glutathione S-transferase A1 (GSTA1) genotype and potential modifiers on breast cancer risk

Effects of glutathione S-transferase A1 (GSTA1) genotype and potential modifiers on breast cancer risk

Molecular characterization of zebrafish Gstr1, the only member of teleost-specific glutathione S- transferase class

Glutathione and its related enzymes in the gonad of Nile Tilapia (Oreochromis niloticus)

Contact Info

Product

Resources

About