Expression of glutathione S-transferases in fetal lung and liver tissue from parental strains and F1 crosses between C57BL/6 and BALB/c F1 mice following in utero exposure to 3-methylcholanthrene

Xu, Mian; Moore, Joseph Earle; Leone-Kabler, Sandra; McCoy, Thomas P.; Swank, Adam; Nelson, Garret B.; Ross, Jeffrey A.; Townsend, Alan J.; Miller, Mark Steven

doi:10.1016/j.bcp.2006.03.010

Cited by 5 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only for cytosolic GSTs, the gene expressions have been observed in ES cell-derived hepatic system [24]. The major cGSTs were expressed in the late gestation mouse fetus [36]. Therefore, in present study, the ES cell-derived hepatic …”

Section: Discussionmentioning

confidence: 75%

MAPEG Expression in Mouse Embryonic Stem Cell-Derived Hepatic Tissue System

Zhu

Huang

et al. 2008

Stem Cells and Development

View full text Add to dashboard Cite

The expressions of membrane-associated proteins in eicosanoid and glutathione metabolism (MAPEG), a superfamily involved in both inflammation and cell protection, were investigated in an in vitro system of mouse embryonic stem (ES) cell-derived hepatic tissue. Gene expressions of all MAPEG members were demonstrated in a developmental-dependent manner in the derived hepatic tissue. The protein expression of microsomal glutathione S-transferase 1 (MGST1) was not detected until differentiating day 14. It gradually increased by maturation of hepatic tissue. The microsomes of ES cell-derived hepatic tissue possessed the MGST1-like catalytic activity. However, MGST1 from the microsome preparation could not form dimers as usual when exposed to reactive nitrogen species ONOO. Among the other members in MAPEG, weak expressions of leukotriene C(4) synthase (LTC(4)S) and microsomal prostaglandin E synthase 1 (mPGES-1) were observed. A stable expression of 5-Lipoxygenase activating protein (FLAP) appeared during the entire course of differentiation. MGST2 and MGST3 failed to express in the derived hepatic tissue, although mRNA of them do existed. In conclusion, ES cell-derived hepatic tissue possess MAPEG gene expression features, but not all protein expression could be detected, which helps to understand not only the nature of the tissue derived, but also the fate of bioartificial liver system, and may as well provide a valuable in vitro model for research in both inflammation process and toxic events in hepatological fields.

show abstract

Section: Discussionmentioning

confidence: 75%

MAPEG Expression in Mouse Embryonic Stem Cell-Derived Hepatic Tissue System

Zhu

Huang

et al. 2008

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…A significant increase in GST activity is observed with the cells induced by 3-methylcholanthrene (MC). In contrast to this study, investigation demonstrated that treatment with methylcholanthrene (MC) had no effect on the levels of GST enzyme activity in either the fetal lung or liver tissue of mice [15]. Decreased activities of GST in the liver of MC-induced tumour bearing mice were also reported [16].…”

Section: Introductionmentioning

confidence: 68%

“…Investigation reported that glutathione-s-transferase catalyses the detoxification of electrophilic diol epoxides produced by the metabolism of polycyclic aromatic hydrocarbons [24]. However, another study did not found any effect of 3-methylcholanthrene on hepatic glutathione-s-transferase activity [15].…”

Section: Discussionmentioning

confidence: 98%

Nandrolone Decanoate Enhances the Activities of Cholanthrene Induced Glutathione-s-Transferase in Liver Tissue of Albino Mice

Acharjee¹,

Mahanta²,

Borkotoky

2010

DML

View full text Add to dashboard Cite

A concrete study is still fragmentary to correlate the effect of Nandrolone decanoate, a anabolic steroid, on the cholanthrene induced Glutathione-s-transferases (GST) activities in liver tissues. Administration of Nandrolone and 3-Methylcholanthrene in alone and combination is found to increase the activity of hepatic GST activity significantly (p< 0.01) suggesting Nandrolone as a potent inducer of GST activity.

show abstract

“…However, the phase II enzymes are relatively uninducible, whereas activating enzymes are highly inducible. This suggests that fetuses are much more susceptible to environmental exposures than adults (41). Genetic polymorphisms in phase II enzymes that further inhibit enzyme activity may exacerbate this susceptibility.…”

Section: Discussionmentioning

confidence: 99%

Variation in the GST mu Locus and Tobacco Smoke Exposure as Determinants of Childhood Lung Function

Breton¹,

Vora²,

Salam³

et al. 2009

Am J Respir Crit Care Med

View full text Add to dashboard Cite

Rationale: The glutathione S-transferases (GSTs) are important detoxification enzymes. Objectives: To investigate effects of variants in GST mu genes on lung function and assess their interactions with tobacco smoke exposure. Methods: In this prospective study, 14,836 lung function measurements were collected from 2,108 children who participated in two Southern California cohorts. For each child, tagging single nucleotide polymorphisms in GSTM2, GSTM3, GSTM4, and GSTM5 loci were genotyped. Using principal components and haplotype analyses, the significance of each locus in relation to level and growth of FEV 1 , maximum midexpiratory flow rate (MMEF), and FVC was evaluated. Interactions between loci and tobacco smoke on lung function were also investigated. Measurements and Main Results: Variation in the GST mu family locus was associated with lower FEV 1 (P 5 0.01) and MMEF (0.04). Two haplotypes of GSTM2 were associated with FEV 1 and MMEF, with effect estimates in opposite directions. One haplotype in GSTM3 showed a decrease in growth for MMEF (2164.9 ml/s) compared with individuals with other haplotypes. One haplotype in GSTM4 showed significantly decreased growth in FEV 1 (251.3 ml), MMEF (269.1 ml/s), and FVC (244.4 ml), compared with all other haplotypes. These results were consistent across two independent cohorts. Variation in GSTM2 was particularly important for FVC and FEV 1 among children whose mothers smoked during pregnancy. Conclusions: Genetic variation across the GST mu locus is associated with 8-year lung function growth. Children of mothers who smoked during pregnancy and had variation in GSTM2 had lower lung function growth.

show abstract

Expression of glutathione S-transferases in fetal lung and liver tissue from parental strains and F1 crosses between C57BL/6 and BALB/c F1 mice following in utero exposure to 3-methylcholanthrene

Cited by 5 publications

References 33 publications

MAPEG Expression in Mouse Embryonic Stem Cell-Derived Hepatic Tissue System

MAPEG Expression in Mouse Embryonic Stem Cell-Derived Hepatic Tissue System

Nandrolone Decanoate Enhances the Activities of Cholanthrene Induced Glutathione-s-Transferase in Liver Tissue of Albino Mice

Variation in the GST mu Locus and Tobacco Smoke Exposure as Determinants of Childhood Lung Function

Contact Info

Product

Resources

About