Expression of glutathione S-transferase placental mRNA in hepatic preneoplastic lesions in rats

Zhu, Hengrui

doi:10.3748/wjg.v4.i1.38

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…To verify the progression of experimental hepatocarcinoma, a total of 10 rats were killed at weeks 10 and 17 (n = 5, each) after the initiation of hepatocarcinogenesis by oral diethylnitrosamine administration. Pathomorphologic observation and histochemical assay using placental glutathione S-transferase (GST-P) staining were done (17).…”

Section: Methodsmentioning

confidence: 99%

Roxithromycin Inhibits Constitutive Activation of Nuclear Factor κB by Diminishing Oxidative Stress in a Rat Model of Hepatocellular Carcinoma

Ueno¹,

Aoki²,

Kubo³

et al. 2005

Clinical Cancer Research

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Purpose: Recently, 14-member macrolide antibiotics such as clarithromycin and roxithromycin have been shown to have anticancer and antiangiogenic effects.We investigated the suppressive effect of roxithromycin on accelerated hepatocellular carcinoma growth in a rat hepatocarcinogenetic model and compared results with effects from TNP-470. Experimental Design: Tumor was induced by oral diethylnitrosamine administration for 17 weeks. Normal saline,TNP-470 (50 mg/kg), or roxithromycin (40 or 100 mg/kg) was administered i.p. thrice per week from week 10 to 17. Results: Carcinomatous tissue growing outside dysplastic nodules and a marked expression of placental glutathione S-transferase were detected in rats with induced carcinogenesis. Tumor growth was accompanied by augmented expression of inducible nitric oxide synthase, activation of nuclear factor nB, and increased lipid peroxidation level. All these effects were absent in animals that received roxithromycin or TNP-470. The inhibitory effect of roxithromycin was dose dependent and no clear differences were noted between groups given roxithromycin 100 mg/kg and TNP-470 50 mg/kg. Conclusions: Our results indicate that roxithromycin inhibits oxidative stress, nitric oxide production, and nuclear factor nB activation induced by experimental hepatocarcinogenesis. The data provide additional evidence for the potential use of roxithromycin in treatment of hepatocellular carcinoma prevention.Hepatocellular carcinoma is one of the most common malignancies in the world, especially in Asia and Africa. The vast majority of patients have preexisting cirrhosis at the time they develop hepatocellular carcinoma. Despite many treatments for this disease in recent decades, its long-term therapeutic outcome remains poor. Prevention seems to be the best strategy in lowering the present incidence of the disease. A preventive effect of an acyclic retinoid on the development of a second tumor after ablation of an original tumor has been shown by one group (1, 2). Conflicting results on the potential preventive effect of IFN have also been reported (3-5). To validate and expand the concept of chemoprevention to other therapeutics, the molecular events that contribute to hepatocarcinogenesis in the liver with cirrhosis need to be identified and targeted.It was reported that tumor growth is angiogenesis dependent by the discovery of angiostatin or endostatin, both of which specifically inhibit endothelial proliferation (6, 7). There are several drugs that have angiogenesis inhibitory properties; however, most of these are restricted by excessive toxicity and limited efficacy. Roxithromycin is a new 14-member macrolide antibiotic that has a wide antibacterial spectrum against pathogens and an immunomodulatory effect. Interestingly, on tumor angiogenesis, Yatsunami et al. (8) reported that roxithromycin at concentrations >20 Amol/L inhibited endothelial cell migration and tube formation. Moreover, we previously reported the strong inhibitory effect of roxithromycin on tumor necro...

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Section: Methodsmentioning

confidence: 99%

Roxithromycin Inhibits Constitutive Activation of Nuclear Factor κB by Diminishing Oxidative Stress in a Rat Model of Hepatocellular Carcinoma

Ueno¹,

Aoki²,

Kubo³

et al. 2005

Clinical Cancer Research

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“…No γ-GT positive liver cell focus, a postulated precancerous marker [54][55][56] was observed in any liver of the variously treated tree shrews in our study. However, different numbers of γ-GT positive liver cells, which scattered mainly around the portal spaces, were observed in each group.…”

Section: Research On the Preventive Effect Of Oltipraz In Tree Shrewmentioning

confidence: 99%

Chemopreventive effect of oltipraz on AFB₁-induced hepatocarcinogenesis in tree shrew model

Qin

et al. 2000

WJG

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“…DEN can be given per-oral or intra-peritoneally, and/or in combination with other agents [47, 76,118]. Mouse models use DEN alone (intra-peritoneal) [119] while rat and hamster models use DEN (either per-oral or intra-peritoneal) in combination with 2-AAF and/or PH [53, 120,121]. For rats, oral preparations are dosed at 1mg per 100g body weight [120] while the intra-peritoneal dose is 15-20mg per 100g body weight [122,123].…”

Section: Chemical A) Choline-deficient Diet Supplemented With Ethionimentioning

confidence: 99%

Liver Injury Models for Induction of Hepatic Oval Cells in Rodents

Tan¹,

Shuh²,

Cohen³

2015

JLRDT

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Expression of glutathione S-transferase placental mRNA in hepatic preneoplastic lesions in rats

Cited by 9 publications

References 8 publications

Roxithromycin Inhibits Constitutive Activation of Nuclear Factor κB by Diminishing Oxidative Stress in a Rat Model of Hepatocellular Carcinoma

Roxithromycin Inhibits Constitutive Activation of Nuclear Factor κB by Diminishing Oxidative Stress in a Rat Model of Hepatocellular Carcinoma

Chemopreventive effect of oltipraz on AFB₁-induced hepatocarcinogenesis in tree shrew model

Liver Injury Models for Induction of Hepatic Oval Cells in Rodents

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Expression of glutathione S-transferase placental mRNA in hepatic preneoplastic lesions in rats

Cited by 9 publications

References 8 publications

Roxithromycin Inhibits Constitutive Activation of Nuclear Factor κB by Diminishing Oxidative Stress in a Rat Model of Hepatocellular Carcinoma

Roxithromycin Inhibits Constitutive Activation of Nuclear Factor κB by Diminishing Oxidative Stress in a Rat Model of Hepatocellular Carcinoma

Chemopreventive effect of oltipraz on AFB1-induced hepatocarcinogenesis in tree shrew model

Liver Injury Models for Induction of Hepatic Oval Cells in Rodents

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Product

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Chemopreventive effect of oltipraz on AFB₁-induced hepatocarcinogenesis in tree shrew model