Expression of gicerin enhances the invasive and metastatic activities of a mouse mammary carcinoma cell line

Tsukamoto, Yasuhiro; Sakaiuchi, Takashi; Hiroi, Satoshi; Furuya, Masaru; Tsuchiya, Seiichiro; Sasaki, Fumihiko; Miki, Naomasa; Taira, Eiichi

doi:10.3892/ijo.23.6.1671

Cited by 9 publications

(10 citation statements)

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“…Like most other cell adhesion molecules in the Ig-superfamily, gicerin has cell adhesive activities with its ligands (8)(9)(10)(11)22). We have previously reported that gicerin in regenerating cells might participate in the reconstruction of injured tissues through its cell adhesion properties; namely, cell-cell connections in the remodeling epithelium are promoted by gicerin-gicerin interaction, while cell-ECM binding is also enhanced by gicerin-NOF interaction (7).…”

Section: Discussionmentioning

confidence: 99%

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Involvement of transforming growth factor-β in the expression of gicerin, a cell adhesion molecule, in the regeneration of hepatocytes

Tsuchiya

Tsukamoto²,

Taira³

et al. 2007

Int J Mol Med

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Abstract. Gicerin, an Ig-superfamily cell adhesion molecule, appears transiently in embryonic tissues including those of the nervous, urogenital, respiratory and digestive systems, and it promotes neurite extension, cell migration and epithelialization through its cell adhesive activities. In addition, gicerin also reappears in regenerating tissue after suffering either a traumatic injury or a viral infection. In the present study, we examined the expression pattern of gicerin in the regeneration of hepatocytes. Immunohistochemically, gicerin protein appeared in the regenerating hepatocytes of carbon tetrachloride (CCl 4 )-induced acute hepatitis, while it was scarcely expressed in the hepatocytes of normal mouse liver. Real-time PCR revealed the up-regulation of gicerin transcription in the regenerating process of CCl 4 -induced hepatitis. The expression of transforming growth factor (TGF)-ß1 was also increased during the regeneration. Furthermore, the gicerin mRNA expression increased during the process of an in vitro hepatocyte regeneration model using mouse primary hepatocytes and hepa 1-6 cells. To note, the mRNA levels of gicerin in these cells were enhanced by the presence of TGF-ß1. Collectively, these findings suggest that TGF-ß1 may therefore regulate gicerin expression in hepatocytes leading to liver regeneration by cell-cell or cell-ECM interactions.

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Section: Discussionmentioning

confidence: 99%

“…In contrast, endothelial cells express gicerin continuously even after maturation. Interestingly, gicerin is overexpressed in some sporadic tumors, and it also promotes binding among tumor cells and the adhesion of tumor cells to ECM, thus leading to tumor progression (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Involvement of transforming growth factor-β in the expression of gicerin, a cell adhesion molecule, in the regeneration of hepatocytes

Tsuchiya

Tsukamoto²,

Taira³

et al. 2007

Int J Mol Med

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“…It is reported that cell adhesion molecules (CAMs) do not only play important roles in keeping cells close contact, but are also involved in pathologic conditions, including tumor progression, invasion, and metastasis (3,4). Periostin, secreted by osteoblasts, shares a structural homology to insect fasciclin I and supports adhesion of osteoblasts (5,6), leading to a hypothesis that it functions to modulate cell-to-matrix interactions and acts in cell adhesion, migration, and growth.…”

Section: Introductionmentioning

confidence: 99%

Circulating levels of periostin may help identify patients with more aggressive colorectal cancer

Ben

Zhao²,

Ge³

et al. 2009

Int J Oncol

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Abstract. Elevated levels of periostin have been implicated as playing important roles in tumor invasion and metastasis in various tissues. Thus, we determined whether serum periostin levels were associated with progression and poor prognosis in colorectal cancer (CRC) patients. We measured serum periostin levels by ELISA in 67 CRC patients and 120 controls. We also evaluated periostin expression in human CRC specimens (n=15) using immunohistochemistry, and measured expression of periostin mRNA in 7 CRC tissue samples, matched normal tissues and in 4 colon cancer cell lines by RT-PCR. We analyzed the relationship between serum levels of periostin and other clinicopathologic characteristics in patients with CRC. The serum levels of periostin in CRC patients (40.9±15.4 ng/ml) were significantly elevated compared to that in healthy volunteers (21.0±7.3 ng/ml, P<0.0001) and benign colorectal polyps or adenomas (22.4±8.5 ng/ml, P<0.0001). Higher preoperative serum levels of periostin in CRC were found to correlate with distant metastasis (P=0.003), advanced-stage disease (stage III/IV, P<0.0001) and poor prognosis. Preoperative serum periostin levels of 15 cases were significantly higher than matched postoperative levels (47.2±13.5 ng/ml vs. 31.3±11.0 ng/ml, P=0.008). Twelve of 15 patients (80%) had positive immunohistochemical periostin staining in CRC samples. Interestingly, periostin mRNA was highly upregulated in CRCs in comparison with matched normal tissues, and no expression of periostin mRNA was detected in 4 colon cancer cell lines. Serum levels of periostin detected by ELISA may be of clinical value in identifying patients who may be at high risk for aggression and metastasis of CRC. Periostin may be produced by the stromal cells surrounding the tumor, but not by the CRC cells themselves.

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“…Gicerinalso has two isoforms: L-gicerin and S-gicerin, which have intracellular tailsof different sizes [11]. In our previous studies, L-gicerin was observed in invasive and metastasizing cancer cells and enhanced the invasive and metastatic activities of neoplastic cells in vivo [12] [13].…”

Section: Introductionmentioning

confidence: 96%

Experimental Therapeutic Trial of an Antibody against the Cell Adhesion Molecule Gicerin for Lymphomas Using a Murine Cell Line

Tsukamoto¹,

Miyagawa²,

Maeda³

2018

JCT

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Chemotherapy, occasionally combined with radiotherapy, is a major method for treating lymphoma but frequently produces side-effects in patients. Thus, novel therapeutics should be developed as an alternative the chemotherapy in lymphoma patients. Although the cell adhesion molecule gicerins are almost entirely absent in most mature tissues, except for muscle and endothelial cells, various neoplastic cells strongly express gicerin in their cell membranes. This suggests the potential function of gicerin in the progression of tumors, including tumor growth, invasion and metastasis to distant organs from primary sites. In the present study, we assessed therapeutic effects of anti-gicerin antibodies on the murine lymphoma cell line YAC1. Gicerin was found to be expressed in the cell membrane of YAC1 cells and promoted the cell adhesion activity of the YAC1 cells on HUVECs, an endothelial cell line. In addition, YAC1 cells were implanted sub-cutaneously in mice in order to examine the therapeutic effects of anti-gicerin antibodies on lymphoma progression in vivo. The anti-gicerin antibodies suppressed and reduced the lymphoma tumor growth in the mice, whereas the growth of tumor mass was not inhibited by pre-immune IgG administrations. YAC1 cells were also implanted intravenously in mice in order to examine the effects of anti-gicerin antibodies on the pulmonary metastasis of lymphoma cells. The metastasis of the YAC1 cells to the lungs was inhibited by the injection of anti-gicerin antibodies. These findings indicate that anti-gicerin antibodies inhibit the progression of prednisolone-resistant lymphoma, making this a promising novel therapeutic method for treating refractory lymphoma cases.

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Expression of gicerin enhances the invasive and metastatic activities of a mouse mammary carcinoma cell line

Cited by 9 publications

References 0 publications

Involvement of transforming growth factor-β in the expression of gicerin, a cell adhesion molecule, in the regeneration of hepatocytes

Involvement of transforming growth factor-β in the expression of gicerin, a cell adhesion molecule, in the regeneration of hepatocytes

Circulating levels of periostin may help identify patients with more aggressive colorectal cancer

Experimental Therapeutic Trial of an Antibody against the Cell Adhesion Molecule Gicerin for Lymphomas Using a Murine Cell Line

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