Expression of ghrelin and ghrelin functional receptor GHSR1a in human pituitary adenomas

Andrusiewicz, Mirosław; Komarowska, Hanna; Skibinska, Izabela; Chmielewska, Małgorzata; Jaskuła-Świtek, Magdalena; Liebert, W; Waśko, Ryszard; Kotwicka, Małgorzata

doi:10.20452/pamw.3967

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…Ghrelin is generated from the preproghrelin precursor during posttranslational cleavage. Ghrelin is predominantly secreted by endocrine cells of the gastric mucosa, but its expression was also found in many other organs, including the small and large intestine, hypothalamus, pituitary, adrenal glands, testes, ovaries, thyroid gland, liver or lungs [8][9][10][11][12][13][14]. The main role of ghrelin is to regulate energy homeostasis and secretion of growth hormone.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical analysis of ghrelin expression in various types of adrenal tumors

Komarowska

Malińska

Komekbai

et al. 2021

Folia Histochem Cytobiol.

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Introduction. Ghrelin, originally isolated from the endocrine cells of the gastric mucosa, is also expressed in many peripheral tissues, including normal adrenals and adrenocortical tumors. It was shown that ghrelin stimulates proliferation and inhibits apoptosis of adrenocortical cells. In the current study, we compared ghrelin expression at the protein level in various adrenal tumors. We analyzed whether immunoreactive ghrelin could be considered as a potential marker for different types of adrenal tumors. Material and methods. Study was carried out on 200 adrenal specimens arranged on microscope slide in tissue microarray format. We performed standardized immunohistochemical reactions with semiquantitative reaction intensity measurements.Results. At the protein level, the expression of ghrelin was significantly reduced in adrenocortical adenocarcinoma in relation to the control group and pheochromocytoma as well as cancer-adjacent normal adrenal tissue. In contrast, a relatively high ghrelin expression was found in pheochromocytoma compared to all analyzed groups, with the exception of cancer-adjacent normal adrenal tissue.Conclusions. The ghrelin expression profile at the protein level may be associated with the type of adrenal tumors. In this context, our results suggest that adrenal immunoreactive ghrelin may be considered as a sensitive and specific marker for differentiating adrenocortical carcinoma from adrenocortical adenoma and pheochromocytoma.

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Section: Introductionmentioning

confidence: 99%

Immunohistochemical analysis of ghrelin expression in various types of adrenal tumors

Komarowska

Malińska

Komekbai

et al. 2021

Folia Histochem Cytobiol.

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“…However, one study found GHS-R1b more expressed in tumor cells with advancing colorectal carcinoma stage while GHS-R1a expression was decreased[ 10 ]. Ghrelin has been investigated in different tumor tissues and although not all results concurred, most were consistent in tumor expression of ghrelin and in favor of its proliferative and anti-apoptotic role[ 11 - 16 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of adipokine ghrelin and ghrelin receptor in human colorectal adenoma and correlation with the grade of dysplasia

Stojsavljević-Shapeski¹,

Virović-Jukić²,

Tomas³

et al. 2021

WJGS

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BACKGROUND Ghrelin is an adipokine that plays an important role in energy balance. Expression of ghrelin and ghrelin receptor has been investigated in different tissues and tumors. Studies regarding expression of ghrelin and ghrelin receptor in colorectal tumors are scarce and no data on expression of ghrelin and its receptor in colorectal adenomas has been published. Ghrelin and ghrelin receptor were highly expressed in colon carcinoma cells while expression was decreased in less differentiated tumors, presuming that ghrelin might be important in early phases of tumorigenesis. AIM To investigate the expression of ghrelin and ghrelin receptor in human colorectal adenomas and adjacent colorectal tissue. METHODS In this prospective study (conducted from June 2015 until May 2019) we included 92 patients (64 male and 28 female) who underwent polypectomy for colorectal adenomas in the Department of Gastroenterology and Hepatology, “Sestre milosrdnice” Clinical Hospital Center in Zagreb, Croatia. After endoscopic removal of colorectal adenoma, an additional sample of colon mucosa in the proximity of the adenoma was collected for pathohistological analysis. Adenomas were graded according to the stage of dysplasia, and ghrelin and ghrelin receptor expression were determined immunohistochemically in both adenoma and adjacent colon tissue using the polyclonal antibody for ghrelin (ab150514, ABCAM Inc, Cambridge, United States) and ghrelin receptor (ab48285, ABCAM Inc, Cambridge, United States). Categorical and nominal variables were described through frequencies and proportions and the difference between specific groups were analyzed with Fisher’s and Fisher-Freeman-Halton’s method respectively. Spearman's rank correlation coefficient was determined for correlation of expression of ghrelin and ghrelin receptor in adenoma and adjacent colon tissue with the grade of adenoma dysplasia. RESULTS Among 92 patients with colorectal adenoma 43 had adenomas with high-grade dysplasia (46.7%). High expression of ghrelin was 7 times more common in high-grade adenoma compared to low-grade adenomas (13.95% to 2.04%, P = 0.048), while the expression of ghrelin in adjacent colon tissue was low. We found no correlation between ghrelin receptor expression in adenoma and adjacent colon tissue and the grade of colorectal adenoma dysplasia. The most significant correlation was found between ghrelin and ghrelin receptor expression in adenomas with high-grade dysplasia (rho = 0.519, P < 0.001). CONCLUSION Ghrelin and ghrelin receptor are expressed in colorectal adenoma and adjacent tissue with ghrelin expression being more pronounced in high grade dysplasia as a possible consequence of increased local synthesis.

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“…Initially, unacylated ghrelin had been considered as the inactive form, but currently it is known that both forms exhibit biological activity. Ghrelin expression has also been demonstrated in a number of organs and tumours . Widely distributed, ghrelin regulates different functions in the body and influences energy homeostasis and GH release .…”

Section: Introductionmentioning

confidence: 99%

Ghrelin as a potential molecular marker of adrenal carcinogenesis: In vivo and in vitro evidence

Komarowska

Ruciński

Tyczewska

et al. 2018

Clinical Endocrinology

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Expression of ghrelin and ghrelin functional receptor GHSR1a in human pituitary adenomas

Cited by 3 publications

References 5 publications

Immunohistochemical analysis of ghrelin expression in various types of adrenal tumors

Immunohistochemical analysis of ghrelin expression in various types of adrenal tumors

Expression of adipokine ghrelin and ghrelin receptor in human colorectal adenoma and correlation with the grade of dysplasia

Ghrelin as a potential molecular marker of adrenal carcinogenesis: In vivo and in vitro evidence

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