Expression of genes related to lipid transport in meat-type ducks divergent for low or high residual feed intake

Jin, Sihua; Xu, Yuan; Zang, He; Yang, Lei; Lin, Zhiqiang; Li, Yongsheng; Zhao, Geng

doi:10.5713/ajas.19.0284

Cited by 8 publications

(8 citation statements)

References 35 publications

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“…The FABP1 is a highly-expressed DEG in the OJF and downregulated in IJF in the current study. The APOH protein is involved with triggering of lipoprotein lipase in lipid metabolism 26,27 . The ALDOB gene is an important gene in mammals' metabolisms and homeostasis.…”

Section: Discussionmentioning

confidence: 99%

“…ZN37A is one of zinc nger proteins (ZFPs), a direct study on this lipid's metabolism has not been found, however ZFPs are involved with lipid bindings and lipid and glucose metabolism 29,30 . ALBU is also identi ed as a biomarker for lipid-related diseases in human-like obesity 27,31 . SLC1A2 and SLC2A2 are solute carrier associated proteins which are involved in lipid biosynthesis, fatty acid metabolic process and fatty acid transport 32-35 .…”

Section: Discussionmentioning

confidence: 99%

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Potential Candidate Genes and Pathways Affecting the Metabolism of Jaw Fats in Risso’s Dolphin: Based on Transcriptomics

Senevirathna

Yonezawa

Igarashi

et al. 2021

Preprint

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Jaw fats play a key role in echolocation in toothed whales. These fats are located along the outer and inner segments of the lower jawbone. A ribose nucleic acid (RNA) sequencing technique was employed to investigate transcriptomes of these two types of jaw fat tissues in Risso’s dolphins. We identified 1,899 upregulated common genes in both fat tissues. The differentially-expressed genes (DEGs) analysis showed that 34 and nine known genes were significantly upregulated in outer and inner jaw fats, respectively. A functional enrichment analysis was conducted by Enricher; lipid metabolism-related gene ontologies (GO) and pathways were identified (p<0.05). Based on these analyses, APOH, HNF4A, MYF6, SLC1A2, SLC2A2 and ALDOB were key genes for lipid metabolism in the outer jaw fat which are mainly involved with lipoprotein lipase activities. However, APP, DHX9, PXMP4 and THBS4 genes were highly expressed in the inner jaw fat, and their main functional enrichments were amyloid-beta formation and the activation of ECM-receptor interaction. These recent findings provide evidence for de novo lipid synthesis and as a new concept, the APP may be involved with transferring sound wave signals from the inner jaw fat to the brain via neurons, and further studies are necessary for revealing the puzzle of echolocation in toothed whales.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Potential Candidate Genes and Pathways Affecting the Metabolism of Jaw Fats in Risso’s Dolphin: Based on Transcriptomics

Senevirathna

Yonezawa

Igarashi

et al. 2021

Preprint

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“…Recent studies have found that ALB expression was strongly correlated with the feed efficiency of meat‐type ducks (Jin et al . 2020). It may be that ALB is also related to the biological process of cell response to starvation.…”

Section: Discussionmentioning

confidence: 99%

A joint analysis strategy reveals genetic changes associated with artificial selection between egg‐type and meat‐type ducks

Zhu

et al. 2020

Animal Genetics

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Egg-type ducks and meat-type ducks are predominantly commercial or indigenous and have been subjected to artificial directional selection. These two duck types differ substantially in body shape, production performance and reproductivity. However, the genetic changes associated with phenotypic differences remain unclear. Here, we compared the two duck types at the genomic and transcriptomic levels. We identified a large number of SNPs and genes in genomic divergent regions in terms of F ST and hp values. The corresponding genes were mainly enriched in embryonic development function and metabolic pathway. RNAseq analysis also revealed differential gene expression in the liver and gonads. The differentially expressed genes were functionally associated with signal transmission and substance metabolism respectively. Furthermore, we found that seven genes were related to differentiation between the two types by both g genome and transcriptome analysis and were plausible candidate genes. These genes were annotated to GO categories of cell development and disease immunity. These findings will enable a better understanding of the artificial selection history of meat and egg ducks and provide a valuable resource for future research on the breeding of these two lineages.

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“…When cells grew to 80% confluence, they were reseeded into 24-well plates for immunofluorescence staining of PAX7 (Proteintech, China) [22,23]. The cells were fixed for…”

Section: Primary Cell Isolation Identification and Culturementioning

confidence: 99%

“…A previous study showed that APOH is the difference gene between high and low groups of meat ducks with residual feed intake, and the factor affecting the residual feed intake of meat ducks is lipid metabolism [ 22 ]. Therefore, the APOH gene may play an important role in duck lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts

Pan¹,

Du²,

Li³

et al. 2022

J Anim Sci Technol

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Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway.This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction.

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Expression of genes related to lipid transport in meat-type ducks divergent for low or high residual feed intake

Cited by 8 publications

References 35 publications

Potential Candidate Genes and Pathways Affecting the Metabolism of Jaw Fats in Risso’s Dolphin: Based on Transcriptomics

Potential Candidate Genes and Pathways Affecting the Metabolism of Jaw Fats in Risso’s Dolphin: Based on Transcriptomics

A joint analysis strategy reveals genetic changes associated with artificial selection between egg‐type and meat‐type ducks

Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts

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