Expression of genes encoding subunits A and B of succinate dehydrogenase in germinating maize seeds is regulated by methylation of their promoters

Епринцев, А. Т.; Федорин, Д. Н.; Karabutova, L. A.; Igamberdiev, Abir U.

doi:10.1016/j.jplph.2016.08.008

Cited by 28 publications

(13 citation statements)

References 36 publications

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“…The expression of aconitase and fumarase genes is regulated by the methylation of promoters of the genes encoding the mitochondrial and the cytosolic forms of fumarase. A similar mechanism of regulation of TCA cycle enzymes was shown earlier for the anoxic conditions [30][31][32].…”

Section: Discussionsupporting

confidence: 78%

Effect of Salt Stress on the Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Aconitase and Fumarase in Maize

Епринцев

Федорин

Cherkasskikh

et al. 2021

IJMS

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The influence of salt stress on gene expression, promoter methylation, and enzymatic activity of the mitochondrial and cytosolic forms of aconitase and fumarase has been investigated in maize (Zea mays L.) seedlings. The incubation of maize seedlings in 150-mM NaCl solution resulted in a several-fold increase of the mitochondrial activities of aconitase and fumarase that peaked at 6 h of NaCl treatment, while the cytosolic activity of aconitase and fumarase decreased. This corresponded to the decrease in promoter methylation of the genes Aco1 and Fum1 encoding the mitochondrial forms of these enzymes and the increase in promoter methylation of the genes Aco2 and Fum2 encoding the cytosolic forms. The pattern of expression of the genes encoding the mitochondrial forms of aconitase and fumarase corresponded to the profile of the increase of the stress marker gene ZmCOI6.1. It is concluded that the mitochondrial and cytosolic forms of aconitase and fumarase are regulated via the epigenetic mechanism of promoter methylation of their genes in the opposite ways in response to salt stress. The role of the mitochondrial isoforms of aconitase and fumarase in the elevation of respiration under salt stress is discussed.

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Section: Discussionsupporting

confidence: 78%

Effect of Salt Stress on the Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Aconitase and Fumarase in Maize

Епринцев

Федорин

Cherkasskikh

et al. 2021

IJMS

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“…Another study on transgenic flax crop showed that the gene chalcone synthase was more accessible to DNA methylation when located within CpG islands and the expression of the gene depended on the methylation status in these regions (Dzialo et al ., 2017). The promoter of succinate dehydrogenase gene ( sdh2-1 ) showed increased methylation but highly reduced gene expression on the eighth day of germination in the scutellum of maize seeds (Eprintsev et al ., 2016). These reports as well as the present study provided a lead to argue that CpG islands may play a role in modulating the gene expression in plants.…”

Section: Discussionmentioning

confidence: 99%

Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice

Sapna¹,

Ashwini²,

Ramesh³

et al. 2020

Plant Genet. Resour.

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DNA methylation is known to regulate gene expression when plants are exposed to abiotic stress such as drought. Therefore, insight into DNA methylation pattern would be useful for a better understanding of the expression profile of genes associated with drought adaptation. In the present study, we attempted to analyse the DNA methylation pattern at the whole-genome level and the expression of a few drought-responsive genes in rice under different regimes of soil water status, i.e. puddled, 100 and 60% field capacities (FC). The methylation-sensitive randomly amplified polymorphic DNA analysis was employed to identify DNA methylation pattern. We observed an increase in DNA methylation at 60% FC, and reduced methylation under 100% FC compared to puddled condition. The genes such as protein phosphatases (PP2C) and phenylalanine ammonia-lyase (PAL) having CpG islands in their promoter region had lower expression level under 100 and 60% FC compared to puddled conditions. Heat shock protein 70 (HSP70) and RNA helicase 25 (RH25), with no CpG islands in their promoter region, exhibited enhanced expression compared to puddled plants. In rice, increased DNA methylation seems to be an important mechanism associated with drought responses, which probably regulates the methylation-sensitive gene expression. The drought-induced changes in DNA methylation would contribute for epigenetic mechanism. The study provided evidence to argue that drought-induced increased methylation might be one of the major mechanisms associated with acclimation responses in field crops like rice.

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“…Due to hypermethylation in the promoter region of SDH2-2, the expression of this gene was reduced in both the stem apex and buds of the high-TPC lines. Indeed, increased methylation in the promoter region affects the expression of SDH2-2 in maize seeds, thereby affecting the glyoxylic acid cycle and seed germination [53]. Therefore, the differential methylation of the SDH2-2 promoter region causes signi cant differences in gene expression between the high-and low-TPC materials, thereby affecting the glyoxylic acid cycle, leading to differences in the characteristics of the two groups of materials.…”

Section: Discussionmentioning

confidence: 99%

MiRNA-mediated Changes in DNA Methylation Affect the Expression of Genes Involved in the Thickness of Pod Canopy Trait in Brassica Napus

Chen

Jia

Wan

et al. 2021

Preprint

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Background: Methylation plays an important role in regulating crop development, but little is known about how methylation regulates plant architecture in rapeseed (Brassica napus). Here, we examined how methylation affects the TPC (thickness of pod canopy) trait in rapeseed by performing genome-wide methylation analysis of two extreme TPC lines. Results: We detected significant differences in overall methylation levels between the high- and low-TPC lines in the CG, CHG, and CHH contexts in the promoters of genes in the stem apex and flower bud. In flower buds, 26 genes had significantly higher methylation levels in the high-TPC samples compared to the low-TPC samples, resulting in significantly reduced gene expression. By contrast, in the stem apex samples, the promoter regions of 22 genes were hypermethylated in the high- vs. low-TPC samples. The promoters of 19 and 21 genes had significantly reduced methylation levels in the flower bud and stem apex, respectively, of the high- vs. low-TPC samples, resulting in significantly higher expression levels. Some of these differentially expressed genes are associated with TPC-related traits, such as BnaC03g53050D (UBC32), BnaA05g26660D (CYSB), BnaA10g07880D (TCP 1), BnaAnng09670D (SMP1), BnaA09g02000D (SDH2-2), BnaC01g12960D (NRT1.8), and BnaC09g30490D (TAF15b). In addition, 14 important genes related to growth and development were differentially regulated between the two groups due to miRNA-mediated differences in methylation levels in their promoters. For example, hypermethylation in the promoter region of BnaCnng64040D (Lipase family protein) mediated by miR159a led to significantly reduced gene expression in flower buds of high-TPC vs. low-TPC lines. Conclusions: These results, together with our previously generated RNA-seq and miRNA profiling data, indicate that both methylation and miRNAs are involved in regulating the expression of genes in nitrogen-related metabolic pathways, thereby affecting the TPC trait in B. napus, providing a reference for uncovering the molecular mechanism regulating this crucial trait.

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Expression of genes encoding subunits A and B of succinate dehydrogenase in germinating maize seeds is regulated by methylation of their promoters

Cited by 28 publications

References 36 publications

Effect of Salt Stress on the Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Aconitase and Fumarase in Maize

Effect of Salt Stress on the Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Aconitase and Fumarase in Maize

Assessment of DNA methylation pattern under drought stress using methylation-sensitive randomly amplified polymorphism analysis in rice

MiRNA-mediated Changes in DNA Methylation Affect the Expression of Genes Involved in the Thickness of Pod Canopy Trait in Brassica Napus

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