Expression of genes coding for chromatin-modifying enzymes in maize embryo sacs before and after pollination

Volokhina, I. V.; Gusev, Yu. S.; Moiseeva, Yelizaveta; Fadeev, V. V.; Kolesova, A. Yu.; Gutorova, O. V.; Chumakov, M. I.

doi:10.1016/j.plgene.2020.100221

Cited by 3 publications

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“…There also were ESs with additional cells and nuclei in the egg apparatus, synergide-like cells, and ESs with two-celled proembryos or additional egg cells (data not shown). The frequency of spontaneous proembryo formation in the AT-4 line was 10 times higher than the number of parthenogenic proembryos found earlier in the parthenogenic AT-3 line [ 33 ].…”

Section: Resultsmentioning

confidence: 98%

“…The isolated ESs were stained with a glycerol–acetocarmine solution, as described by Volokhina et al [ 33 ]. The ESs were analyzed with a DM2500 light fluorescence microscope (Leica Microsystems, Wetzlar, Heese, Germany) at the Simbioz Center for the Collective Use of Research Equipment in the Field of Physical–Chemical Biology and Nanobiotechnology (Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov).…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we examined for the first time the expression of the CME genes in the female gametophytes of the AT-3 line and have found no large differences between the ESs of the parthenogenic line AT-3 and the ordinary line DHH-1 before and after pollination, except that the expression of the hon101 and hdt104 genes was higher in DHH-1 [ 33 ]. However, there are no data on maize gene expression in parthenogenic proembryos or in unpollinated ESs.…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression in Parthenogenic Maize Proembryos

Volokhina

Gusev

Moiseeva

et al. 2021

Plants

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Angiosperm plants reproduce both sexually and asexually (by apomixis). In apomictic plants, the embryo and endosperm develop without fertilization. Modern maize seems to have a broken apomixis-triggering mechanism, which still works in Tripsacum and in Tripsacum–maize hybrids. For the first time, maize lines characterized by pronounced and inheritable high-frequency maternal parthenogenesis were generated 40 years ago, but there are no data on gene expression in parthenogenic maize proembryos. Here we examined for the first time gene expression in parthenogenic proembryos isolated from unpollinated embryo sacs (ESs) of a parthenogenic maize line (AT-4). The DNA-methylation genes (dmt103, dmt105) and the genes coding for the chromatin-modifying enzymes (chr106, hdt104, hon101) were expressed much higher in parthenogenic proembryos than in unpollinated ESs. The expression of the fertilization-independent endosperm (fie1) genes was found for the first time in parthenogenic proembryos and unpollinated ESs. In parthenogenic proembryos, the Zm_fie2 gene was expressed up to two times higher than it was expressed in unpollinated ESs.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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