Expression of genes belonging to the IGF-system in glial tumors

Dmitrenko, V. V.; Kavsan, V. M.; Boyko, O. I.; Rymar, V. I.; Stepanenko, Aleksei A.; Balynska, Olena V.; Mausheva, T. A.; Rozumenko, Volodymyr; Zozulya, Yuriy

doi:10.3103/s0095452711050021

Cited by 4 publications

(1 citation statement)

References 56 publications

(61 reference statements)

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“…In this tumor, as well as in teratocarcinoma derived of PCC stem cells, the IGF-I and insulin increases proliferation by different signalling pathways, promoting cell growth of glioblastoma [79]. By the way, the clinical epidemiologic studies evidenced an increase mortality rate in patients treated with insulin [80], underlying the supplement data of signal transdution pathway of IGF-I playing a role in risk factor of the cancer development [79,81]. A serum level of IGF-I is higher in glioblastoma patients [82], and tumor tissues present an increased IGF-I and insulin receptors levels compared to normal tissue [83] inducing the proliferation, differentiation and migration of tumor cells [83,84].…”

Section: Stem Cells Neural and Glial Cells In Neoplasia: Therapeutic Implicationsmentioning

confidence: 99%

Brain stem cells and IGF-I: implications in development, regeneration and cancer therapeutics

Bueno¹,

Santander²,

Alvarez³

et al. 2018

Integr Mol Med

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For the past 40 years, our challenge has been finding a solution for the treatment of brain tumors using our knowledge of the evolution of life, the chemistry of proteins, genetics, and molecular biology. Efficient strategies were established by the construction of vectors targeting oncoproteins or growth factors (i.e. IGF-I, AFP) present during the development of the embryonic and fetal nervous system tissues originating from neural stem cells. The neoplastic stem cells, PCC3 and PCC4, derived from mouse teratocarcinoma -tumor mimicking the structures of developing central nervous system -were transfected with the vectors expressing either antisense IGF-I RNA or IGF-I RNA forming a triple helix RNA-DNA. Both approaches completely stopped the synthesis of the IGF-I growth factor and converted the stem cells into immunogenic cells expressing MHC-I and B7. The immunogenic anti IGF-I transfected cells became antitumor vaccines. The role played by stem cells in the nervous system has motivated us in the search for knowledge focused on self-regeneration and therapeutic strategies. The strategy of Anti IGF-I vaccines was applied with success for therapy of glioblastoma.

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Section: Stem Cells Neural and Glial Cells In Neoplasia: Therapeutic Implicationsmentioning

confidence: 99%

Brain stem cells and IGF-I: implications in development, regeneration and cancer therapeutics

Bueno¹,

Santander²,

Alvarez³

et al. 2018

Integr Mol Med

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Factors and their receptor genes in glioma cells with suppressed function of ERN 1 enzyme upon glucose deprivation

Kharkova

Minchenko

2016

BTSNUKSPPFR

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It was shown that the expression level of insulin like growth factors (IGF1 and IGF2) genes is decreased, but IGF receptor (IRF1R) gene is significantly increased in U87 glioma cells with suppressed activity of the sensor and signaling enzyme ERN1. In U87 glioma cells the expression level of IGF1 gene is decreased but IGF2 and IGF1R do not change significantly upon glucose deprivation condition. The inhibition of ERN1 functional activity does not affect the sensitivity of IGF1 and IGF1R gene expressions to glucose deprivation but the inhibition of ERN1 eliminates the effect of glucose deprivation on IGF2 gene expression. Thus, the IGF1, IGF2 and IGF1R genes are related to the regulation of glioma cells proliferation and are sensitive to glucose deprivation in dependence of ERN1 enzyme function.

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Characterization of new cell line stably expressing CHI3L1 oncogene

et al. 2011

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Aim. To characterize the immortalized 293 cell line after stable transfection with human oncogene (CHI3L1). Methods. 293 cells, stably transfected with pcDNA3.1_CHI3L1, and 293 cells, stably transfected with pcDNA3.1 as a negative control, were used throughout all experiments. The clones of CHI3L1-expressing 293 cells and 293 cells, transfected with pcDNA3.1, were analyzed by immunofluorescence and confocal microscopy. Cell proliferation was measured using MTT assay; analyses of ERK1/2 and AKT activation and their cellular localization were performed with anti-phospho-ERK and anti-phospho-AKT antibodies. Specific activation of MAP and PI3 kinases was measured by densitometric analysis of Western-blot signals. Results. The obtained results show quite modest ability of CHI3L1 to stimulate cell growth and reflect rather an improved cellular plating efficiency of the 293 cells stably transfected with pcDNA3.1_CHI3L1 as compared to the 293 cells transfected with an «empty» vector. ERK1/2 and AKT are activated in the 293_CHI3L1 cells. In these cells phosphorylated ERK1/2 were localized in both cell cytoplasm and nuclei while AKT only in cytoplasm. The 293_CHI3L1 cells differed from the 293 cells, transfected with an «empty» vector, in their size and ability to adhere to the culture plates. Conclusions. The overexpression of CHI3L1 is likely to have an important role in tumorigenesis via a mechanism which involves activation of PI3K and ERK1/2 pathways. The tumors which can be induced by orthotopic implantation of the transformed human cells with overexpressed human oncogene CHI3L1 into the rat brain can be used as a target for anticancer drug development

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Expression of genes belonging to the IGF-system in glial tumors

Abstract: Increased expression of the insulin like growth factor (IGF)

Cited by 4 publications

References 56 publications

Brain stem cells and IGF-I: implications in development, regeneration and cancer therapeutics

Brain stem cells and IGF-I: implications in development, regeneration and cancer therapeutics

Factors and their receptor genes in glioma cells with suppressed function of ERN 1 enzyme upon glucose deprivation

Characterization of new cell line stably expressing CHI3L1 oncogene

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