Expression of gap junctional protein connexin43 during 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis

Xia, Juan; Liu, Xiumei; Tao, Xiaoan; Hong, Yun; Chen, Xiaobing; Dai, Yaohui; Huang, Yulei; Cheng, Bin

doi:10.1007/s10735-009-9229-y

Cited by 6 publications

(7 citation statements)

References 30 publications

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“…Our previous study indicated that TAOS1 and EMS1 amplifications and downregulation of Cx43 might be involved in oral carcinogenesis (Xia et al 2007(Xia et al , 2009. Chemokines are involved in cellular interactions and tropism in situations frequently associated with inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…Leukoplakic oral epithelia provide a useful model for exploring the molecular mechanisms of oral carcinogenesis (Xia et al 2007(Xia et al , 2009. In this study, we set out to evaluate the differential gene expressions of chemokines and chemokine receptors by microarray in surgically resected OLK specimens with epithelial dysplasia and normal epithelia, and validate our microarray results by real-time RT-PCR.…”

Section: Introductionmentioning

confidence: 99%

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Expressions of CXCR7/ligands may be involved in oral carcinogenesis

et al. 2011

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Oral carcinogenesis is a multistep process and requires accumulation and interplay of a series of molecular events. Chemokines and their receptors have been suggested to play important roles in the initiation or progression of cancers. Until now, no report focuses on their alterations in premalignant stage of oral squamous cell carcinoma (OSCC). Compared with normal tissues, mRNA levels of 9 chemokines and 3 chemokine receptors including CXCR7 in oral leukoplakia (OLK) were increased more than two folds by microarray analysis. Then, CXCR7 was selected for further confirmation and immunohistochemistry examination during multistage oral carcinogenesis. CXCR7 was expressed in 85% of OLK and 86% of OSCC. However, only 8% (1 of 13 cases) of normal tissue displayed CXCR7 immunostaining. The positive ratios of CXCR7, CXCL12 and CXCL11 in OLK and OSCC tissues respectively, were significantly higher than that in normal epithelia (P < 0.05), although no significant difference was found between OLK and OSCC. Meanwhile, CXCR7 always concomitantly expressed with it ligands in OLK and OSCC tissues. Our results indicated that CXCR7-CXCL12/CXCL11 axis might play important roles in oral carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expressions of CXCR7/ligands may be involved in oral carcinogenesis

et al. 2011

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“…It was recently suggested that post-transcriptional, translational and degradation regulations play a similarly important gene transcription role in the determination of protein concentrations (33). Despite reduced Cx43 protein levels, normal high mRNA levels were detected (24). Budunova et al (34) investigated the expression of Cxs 26, 43 and 31.1 in mouse hyperplastic skin, papilloma and SCC.…”

Section: Descriptive ------------------------------------------------mentioning

confidence: 99%

“…In addition to significantly different expression patterns between the studied tissue types, high membrane Cx43 expression in OSCC tissues was found to be associated with poor prognosis (23). Xia et al (24) previously reported reduced Cx43 protein concentration, despite normal mRNA levels, in an induced rat tongue carcinogenesis model. The present study aimed to confirm the expression of the described Cx subtypes at the mRNA level by conducting a reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis in 15 tissue sample pairs of OSCC and corresponding oral mucosa.…”

Section: Introductionmentioning

confidence: 96%

Connexin subtype expression during oral carcinogenesis: A pilot study in patients with oral squamous cell carcinoma

Brockmeyer

Hemmerlein

Jung

et al. 2015

Molecular and Clinical Oncology

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Abstract. Gap junctional intercellular communication (GJIC) and connexin (Cx) expression were reported in association with carcinogenesis in various types of tumours. In an earlier histomorphometric study, the protein levels of Cx subtypes 26, 43 and 45 were differentially expressed in oral squamous cell carcinoma (OSCC), corresponding lymph node metastases and dysplasia-free oral mucosa. Moreover, membrane Cx43 acted as an independent prognostic marker in OSCC tissues. This study aimed to confirm the expression of described Cx subtypes at the mRNA level. Hence, a reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis of Cx26, Cx43 and Cx45 gene expressions was performed in paired carcinoma and mucosa samples of 15 OSCC patients. Additionally, we assessed the interaction between Cx subtype expression and clinicopathological routine parameters. The RT-qPCR analysis revealed that Cx26 was downregulated in OSCC (P=0.01), while Cx43 was marginally upregulated in cancer tissue (P=0.04). Cx45 was significantly overexpressed in OSCC tissue compared with the intraoral mucosa controls (P<0.01), and remained unchanged at different tumour stages. No significant interactions between differential Cx subtype expression and clinicopathological routine parameters were observed. In conclusion, Cx regulation at the transcriptional level appears to be an early event during the initiation and development of OSCC, and is maintained during further progression. However, the mRNA-protein correlation is variable. This may be indicative of post-transcriptional, translational and degradation regulations being associated with the determination of Cx protein concentration during oral carcinogenesis.

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“…Recently, Frank et al (12) reported that human tongue squamous cell carcinoma cells were deficient in Cx43 expression. Our previous study showed that Cx43 expression decreased during 4-nitroquinoline-1-oxide–induced rat carcinogenesis (13). These results indicate that OSCC has aberrant GJIC.…”

Section: Introductionmentioning

confidence: 99%

All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

Wang¹,

Dai²,

Huang³

et al. 2013

Med Oral

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Objective: All-trans retinoic acid (ATRA) has been demonstrated to inhibit tumor growth by restoration of gap junctional intercellular communication (GJIC) via upregulation of connexin (Cx) expression in some solid tumors. However, the relationship between ATRA and GJIC remains unclear in oral squamous cell carcinoma (OSCC). The aim of this study was to investigate the effect of ATRA on the GJIC function of OSCC. Study design: We measured the effects of ATRA on the viability and cell cycle distribution of SCC9 and Tca8113 OSCC cells. The GJIC function was observed using the scrape-loading dye transfer technique, and the mRNA and protein levels of Cx32 and Cx43 were detected by qRT-PCR, Western blot, and immunofluorescence assays. Results: ATRA inhibited the growth of OSCC cells in a dose- and time-dependent manner (P <0.05) and caused cell cycle arrest. ATRA-treated cells showed a 2.69-fold and 2.06-fold enhancement of GJIC in SCC9 and Tca8113 cells, respectively (P <0.05). Moreover, ATRA induced upregulation of Cx32 and Cx43 at both the mRNA and protein levels in OSCC cells. Conclusion: Our results indicated that restoration of GJIC via enhanced Cx32 and Cx43 expression might serve as a novel mechanism for the anti-tumor effect of ATRA in OSCC. Key words:All-trans retinoic acid, oral squamous cell carcinoma, connexin, gap junctional intercellular communication.

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Expression of gap junctional protein connexin43 during 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis

Cited by 6 publications

References 30 publications

Expressions of CXCR7/ligands may be involved in oral carcinogenesis

Expressions of CXCR7/ligands may be involved in oral carcinogenesis

Connexin subtype expression during oral carcinogenesis: A pilot study in patients with oral squamous cell carcinoma

All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

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