Expression of gangliosides in an immortalized neural progenitor/stem cell line

Suetake, Keiji; Liour, Sean S.; Tencomnao, Tewin; Yu, Robert K.

doi:10.1002/jnr.10802

Cited by 12 publications

(17 citation statements)

References 41 publications

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“…Furthermore, transfection of several cell lines with ST6GalNAc VI revealed an increase in disialyl Le a expression along with a reduction in monosialyl Le a expression (Tsuchida et al, 2003;Miyazaki et al, 2004;Suetake et al, 2003). Furthermore, the transfection altered the adhesive properties of the cells by lowering their adhesion to E-selectin while increasing their binding to sialic acid binding Ig-like lectin 7 (Siglec-7) (Miyazaki et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Jaluria

Betenbaugh

Κωνσταντόπουλος

et al. 2007

Metabolic Engineering

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The ability to modify cellular properties such as adhesion is of interest in the design and performance of biotechnology-related processes. The current study was undertaken in order to evaluate the effectiveness of modulating cellular adhesion in HeLa cells from a genomics perspective. Using DNA microarrays, differences in gene expression between two phenotypically distinct, anchoragedependent and anchorage-independent, HeLa cell lines were identified. With the aid of several statistical methods and an extensive literature search, two genes were selected as potential targets for further study: siat7e and lama4.Subsequently, experiments were carried out to investigate the effects of siat7e and lama4 separately, on adhesion in HeLa cells by altering their expression in vivo. Decreasing the expression of siat7e, a type II membrane glycosylating sialyltransferase, in anchorage-independent HeLa cells using short interfering RNA (siRNA) resulted in greater aggregation (i.e. clumping) and morphological changes as compared to untreated anchorage-independent HeLa cells. Similar effects were seen in anchorageindependent HeLa cells when the expression of lama4 which encodes laminin α4, a member of the laminin family of glycoproteins, was enhanced as compared to untreated anchorage-independent HeLa cells. Using a shear flow chamber, an attachment assay was developed; illustrating either increased expression of siat7e or decreased expression of lama4 in anchorage-dependent HeLa cells reduced cellular adhesion.Collectively, the results of this study are consistent with the roles siat7e and lama4 play in adhesion processes in vivo and indicate modifying the expression of either gene can influence adhesion in HeLa cells. The strategy of applying bioinformatics techniques to characterize and manipulate phenotypic behaviors is a powerful tool for altering the properties of various cell lines for desired biotechnology objectives.

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Section: Discussionmentioning

confidence: 99%

Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Jaluria

Betenbaugh

Κωνσταντόπουλος

et al. 2007

Metabolic Engineering

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“…In C17.2 cells, a mouse neonatal cerebellar NSC line (Ryder et al. 1990) expressing only a‐series gangliosides due to the lack of GD3‐synthase (GD3S) (Suetake et al. 2003), over‐expression of GD3S leads to repression of epidermal growth factor (EGF) ‐induced cell proliferation (Yanagisawa et al.…”

Section: Glycoconjugates and Signal Transduction In Nscsmentioning

confidence: 99%

Glycosignaling in neural stem cells: involvement of glycoconjugates in signal transduction modulating the neural stem cell fate

Yanagisawa

2007

Journal of Neurochemistry

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The mammalian CNS is organized by a variety of cells, such as neurons and glia, which are generated from neural stem cells (NSCs), undifferentiated neural cells characterized by their high proliferative potential while retaining their capacity for selfrenewal and multipotency. Various signals from the environment, such as the 'niche,' modulate the fate of NSCs in their ability for self-renewal, proliferation, differentiation, and survival. There is increasing evidence that glycoconjugates, including proteoglycans, glycoproteins, and glycolipids, which are part of the plasma membrane glycocalyx network, are involved in mediation of these signals. In the present review, we discuss the roles of glycoconjugates in regulating the fate of NSCs and in supporting the underlying signal transduction mechanisms.

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“…Previously, we characterized the expression of gangliosides in a C17.2 cell line (Suetake et al . 2003).…”

mentioning

confidence: 99%

“…Interestingly, these cells express only a‐series gangliosides, such as GM3, GM2, GM1 and GD1a; they do not express b‐ and c‐series complex gangliosides due to the lack of expression of CMP‐sialic acid : GM3 sialyltransferase (GD3‐synthase, ST‐II, Fig. 1a) (Suetake et al . 2003).…”

mentioning

confidence: 99%

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Involvement of gangliosides in proliferation of immortalized neural progenitor cells

Yanagisawa¹,

Liour²,

Yu³

2004

Journal of Neurochemistry

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The CNS consists of neuronal and glial cells generated from common neural progenitor cells during development. Cellular events for neural progenitor cells, such as proliferation and differentiation, are regulated by multiple intrinsic and extrinsic cell signals. Although much is known on the importance of the proteinous factors in regulating the fate of neural progenitor cells, the involvement of other molecules such as gangliosides, sialic acid-containing glycosphingolipids, remains to be clarified. To elucidate the biological functions of gangliosides in neural progenitor cells, we transfected an immortalized neural progenitor cell line, C17.2, which does not express GD3 ganglioside, with a fusion protein of GD3-synthase (ST-II) and enhanced green fluorescent protein (ST-II-EGFP). Analysis of the ST-II transfectants revealed the ectopic expression of b-and c-series gangliosides. In the ST-II transfectants, proliferation induced by epidermal growth factor (EGF) was severely retarded. EGF-induced proliferation of C17.2 cells was dependent on the Ras-mitogen-activated protein kinase (Ras-MAPK) pathway, and the EGF-induced activation of this pathway was significantly repressed in the transfectants. Thus, ST-II overexpression retarded proliferation of C17.2 cells via repression of the Ras-MAPK pathway. The result supports the concept that gangliosides may play an important role in regulating the proliferation of neural progenitor cells.

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Expression of gangliosides in an immortalized neural progenitor/stem cell line

Cited by 12 publications

References 41 publications

Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells

Glycosignaling in neural stem cells: involvement of glycoconjugates in signal transduction modulating the neural stem cell fate

Involvement of gangliosides in proliferation of immortalized neural progenitor cells

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