Expression of Gangliosides GD3 and 3′‐isoLM1 in Autopsy Brains from Patients with Malignant Tumors

Fredman, Pam; Holst, Hans von; Vp, Collins; Dellheden, Birgitta; Svennerholm, L.

doi:10.1111/j.1471-4159.1993.tb05827.x

Cited by 42 publications

(18 citation statements)

References 31 publications

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“…The increase of GD3 was most significant, but the correlation between GD3 and malignancy remains obscure (15,16). In addition, the lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 are reported to be major gangliosides in human gliomas (16)(17)(18). Because some of these glioma-associated gangliosides are rarely expressed or even are absent in normal tissues (19), they are suitable for targeted therapy (20).…”

mentioning

confidence: 99%

Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers

Lou

Wang²,

Yeh³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Glioblastoma multiforme (GBM) is a deadly brain tumor. More than 50% of patients who suffer from GBM die within 15 mo even received all possible medical treatment. In this study we report that the glycolipid stage-specific embryonic antigen-4 (SSEA-4) is highly expressed on the surface of both GBM cells and GBM specimens. We further demonstrate that the growth of GBM tumor is inhibited when anti–SSEA-4 antibody is administered to experimental mice, suggesting a research proof of concept for the treatment GBM and other SSEA-4 + cancers.

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mentioning

confidence: 99%

Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers

Lou

Wang²,

Yeh³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…T his is supported by an earlier study where we found derangem ents in com position of ganglioside pattern in the opposite hem isphere. 18 N orm ally, nervous tissue m ostly reutilizes lipids present in the brain for m etabolism and, if any, only m inor am ounts of lipids are recruited from serum . 2 T he high growth rate of tum our cells dem ands a large am ount of cholesterol.…”

Section: Discussionmentioning

confidence: 99%

Increased levels of cholesterol esters in glioma tissue and surrounding areas of human brain

Nygren

Holst²,

Månsson³

et al. 1997

British Journal of Neurosurgery

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The proliferation of glioma cells requires cholesterol, which could be provided by synthesis within the cells or by uptake of cholesterol esters in particles of Low Density Lipoprotein (LDL). Cholesterol esters and cholesterol were therefore analysed in human glioma tissue, its surrounding areas and serum from 40 patients. The analyses revealed an increased concentration of cholesterol esters up to 100 times (0.1-10 mumol/g) in both tumour-tissue and surrounding areas compared with control material (< 0.1 mumol/g). The analyses also demonstrated that cholesterol esters in tumour tissue eminated mainly from serum. The cholesterol concentrations were significantly lower in tumour tissue compared with surrounding areas as expected. These results indicate that tumour cell proliferation utilises serum derived cholesterol esters presumably carried by LDL particles.

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“…In this study, we demonstrated that all tumor cell lines also expressed GD3 ganglioside, in addition to SGGLs. It is not surprising that GD3 could be a major surface marker of tumors, especially in neural tumors, such as melanoma [42,43] , brain tumors [44] , astrocytoma [45,46] , glioma [47] , neuroblastoma [48] , meningioma [49,50] , and intracranial tumors [51] . Serum antibodies against GD3 or OAc-GD3 have been shown to be elevated in some patients with melanoma [52] .…”

Section: Discussionmentioning

confidence: 99%

Glycosphingolipid Antigens in Neural Tumor Cell Lines and Anti-Glycosphingolipid Antibodies in Sera of Patients with Neural Tumors

et al. 2008

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To characterize biomarkers in neural tumors, we analyzed the acidic lipid fractions of 13 neural tumor cell lines using enzyme-linked immunoabsorbent assay (ELISA) and high-performance thin-layer chromatography (HPTLC) immunostaining. Sulfated glucuronosyl glycosphingolipids (SGGLs) are cell surface molecules that are endowed with the Human Natural Killer-1 (HNK-1) carbohydrate epitope. These glycosphingolipids (GSLs) were expressed in all cell lines with concentrations ranging from 210 to 330 ng per 2 × 10⁶ cells. Sulfoglucuronosyl paragloboside (SGPG) was the prominent species with lesser amounts of sulfoglucuronosyl lactosaminyl paragloboside (SGLPG) in these tumor cell lines as assessed by quantitative HPTLC immunostaining. Among the gangliosides surveyed, GD3 and 9-O-acetylated GD3 (OAc-GD3) were expressed in all tumor cell lines. In contrast, fucosyl-GM1 was not found to restrict to small cell lung carcinoma cells. In addition, we have analyzed serum antibody titers against SGPG, GD3, and OAc-GD3 in patients with neural tumors by ELISA and HPTLC immunostaining. All sera had high titers of antibodies of the IgM isotype against SGPG (titers over 1:3,200), especially in tumors such as meningiomas, germinomas, orbital tumors, glioblastomas, medulloblastomas, and subependymomas. Serum in a patient with subependymomas also had a high anti-SGGL antibody titer of the IgG and IgA types (titers over 12,800). The titer of anti-GD3 antibody was also elevated in patients with subependymomas and medulloblastomas; the latter cases also had a high titer of antibody against OAc-GD3. Our data indicate that certain GSL antigens, especially SGGLs, GD3, and OAc-GD3, are expressed in neural tumor cells and may be considered as tumor-associated antigens that represent important biomarkers for neural tumors. Furthermore, antibody titers in sera of patients with these tumors may be of diagnostic value for monitoring the presence of tumor cells and tumor progression.

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Expression of Gangliosides GD3 and 3′‐isoLM1 in Autopsy Brains from Patients with Malignant Tumors

Cited by 42 publications

References 31 publications

Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers

Stage-specific embryonic antigen-4 as a potential therapeutic target in glioblastoma multiforme and other cancers

Increased levels of cholesterol esters in glioma tissue and surrounding areas of human brain

Glycosphingolipid Antigens in Neural Tumor Cell Lines and Anti-Glycosphingolipid Antibodies in Sera of Patients with Neural Tumors

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