Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress

Liu, YuDong; Zhang, Li; Meng, Sida; Liu, YuFeng; Zhao, Xiaomeng; Pang, Chunpeng; Zhang, HuiDong; Xu, Tongyu; He, Yi; Qi, Mingfang; Li, Tianlai

doi:10.1093/jxb/erz450

Cited by 38 publications

(42 citation statements)

References 61 publications

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“…In the present study, the overexpression of the AdGolS3 gene increased the abundance of galactinol and raffinose in transgenic Arabidopsis plants and conferred tolerance to drought, salt and osmotic stresses. These results therefore corroborate previous reports demonstrating that the overexpression of GolS genes can successfully impart tolerance to multiple abiotic stresses in the models Arabidopsis and tobacco 5 and also in crop species such as rice, soybean, tomato, and poplar 45,46,49,52 . It is worth noting, however, that the natural accumulation of RFOs in mature seeds to protect the embryo from desiccation is considered an antinutritional factor for many grain legumes 13,17 .…”

Section: Overexpression Ofsupporting

confidence: 91%

“…AdGolS3 showed opposite expression behavior between drought-tolerant and drought-sensitive wild Arachis. The overexpression of GolS genes leads to enhanced tolerance to abiotic stresses (drought, salt, heavy metal, cold and heat), by increasing galactinol and RFO contents in transgenic dicots and monocot species 41,[45][46][47][48][49][50][51][52] . These transgenes were isolated from a number of plant species being the AtGolS2 gene from Arabidopsis, the most commonly used.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Vinson

Mota

Porto

et al. 2020

Sci Rep

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Raffinose family oligosaccharides (RFOs) are implicated in plant regulatory mechanisms of abiotic stresses tolerance and, despite their antinutritional proprieties in grain legumes, little information is available about the enzymes involved in RFO metabolism in Fabaceae species. In the present study, the systematic survey of legume proteins belonging to five key enzymes involved in the metabolism of RFOs (galactinol synthase, raffinose synthase, stachyose synthase, alpha-galactosidase, and beta-fructofuranosidase) identified 28 coding-genes in Arachis duranensis and 31 in A. ipaënsis. Their phylogenetic relationships, gene structures, protein domains, and chromosome distribution patterns were also determined. Based on the expression profiling of these genes under water deficit treatments, a galactinol synthase candidate gene (AdGolS3) was identified in A. duranensis. Transgenic Arabidopsis plants overexpressing AdGolS3 exhibited increased levels of raffinose and reduced stress symptoms under drought, osmotic, and salt stresses. Metabolite and expression profiling suggested that AdGolS3 overexpression was associated with fewer metabolic perturbations under drought stress, together with better protection against oxidative damage. Overall, this study enabled the identification of a promising GolS candidate gene for metabolic engineering of sugars to improve abiotic stress tolerance in crops, whilst also contributing to the understanding of RFO metabolism in legume species.

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Section: Overexpression Ofsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Vinson

Mota

Porto

et al. 2020

Sci Rep

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“…Among four tomato GolSs (SlGolS1, SlGolS2, SlGolS3, and SlGolS4) homologous to AnGolS1, only SlGolS2 was cold-inducible, but at low levels, and believed to be a functional homolog of AnGolS1. AnGolS1 showed higher catalytic activity than SlGolS2 [33]. Thus, it is assumable that these features of AnGolS1 such as a higher inducibility and a higher catalytic activity might confer higher cold stress tolerance than SlGolS2.…”

Section: Galactinol Synthase 1 From a Desert Shrubmentioning

confidence: 97%

“…Galactinol synthase1 (GolS1), a galactinol biosynthesis gene associated with stress tolerance, was shown to be cold-inducible in A. nanus [68,69]. Overexpression of A. nanus GolS1 (AnGolS1) in tomato caused an increase of galactinol contents in young leaves under cold stress, activation of ethylene signaling, and induction of ethylene response factors (ERFs), which led to cold tolerance [33]. Among four tomato GolSs (SlGolS1, SlGolS2, SlGolS3, and SlGolS4) homologous to AnGolS1, only SlGolS2 was cold-inducible, but at low levels, and believed to be a functional homolog of AnGolS1.…”

Section: Galactinol Synthase 1 From a Desert Shrubmentioning

confidence: 99%

Natural Genetic Resources from Diverse Plants to Improve Abiotic Stress Tolerance in Plants

Yolcu

Alavilli

Lee

2020

IJMS

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The current agricultural system is biased for the yield increase at the cost of biodiversity. However, due to the loss of precious genetic diversity during domestication and artificial selection, modern cultivars have lost the adaptability to cope with unfavorable environments. There are many reports on variations such as single nucleotide polymorphisms (SNPs) and indels in the stress-tolerant gene alleles that are associated with higher stress tolerance in wild progenitors, natural accessions, and extremophiles in comparison with domesticated crops or model plants. Therefore, to gain a better understanding of stress-tolerant traits in naturally stress-resistant plants, more comparative studies between the modern crops/model plants and crop progenitors/natural accessions/extremophiles are required. In this review, we discussed and summarized recent progress on natural variations associated with enhanced abiotic stress tolerance in various plants. By applying the recent biotechniques such as the CRISPR/Cas9 gene editing tool, natural genetic resources (i.e., stress-tolerant gene alleles) from diverse plants could be introduced to the modern crop in a non-genetically modified way to improve stress-tolerant traits.

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“…Therefore, improving resistance to such adverse environments is extremely important for agriculture. New genes associated with stress resistance have been identi ed, and transgenic plants carrying these genes exhibit greater tolerance to drought stress [2], salt stress [3], cold stress [4], heavy metals [5], paraquat and wounding stress [1].…”

Section: Introductionmentioning

confidence: 99%

GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

Fan

Liu

Cao

et al. 2021

Preprint

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Background: Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. They have an important effect on the stress tolerance of plants by mediating plant responses to environmental stimuli and cell signal transduction pathways. Although the functions of several LTPs in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans. Results: Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids. The cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that overexpression of GsLTP enhanced tolerance to drought and salt stresses. Conclusions: Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

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Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress

Abstract: Galactinol may act as a sugar signal that affects the ethylene signaling pathway and enhances cold tolerance in germinating seeds and young seedlings of tomato.

Cited by 38 publications

References 61 publications

Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Natural Genetic Resources from Diverse Plants to Improve Abiotic Stress Tolerance in Plants

GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

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