Expression of functional recombinant mosquito salivary apyrase: A potential therapeutic platelet aggregation inhibitor

Sun, Dongfeng; McNicol, Archibald; James, Anthony A.; Peng, Zhikang

doi:10.1080/09537100500460234

Cited by 53 publications

(48 citation statements)

References 38 publications

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“…Apyrase is an ectoenzyme that rapidly metabolizes ADP and adenosine triphosphate (ATP) released from activated platelets and endothelial cells, thereby minimizing ADP-dependent platelet activation and recruitment. 38 Apyrase treatment had no effect on platelet accumulation in anti-GBM antibody-treated mice ( Figure 9A). In contrast, apyrase treatment significantly decreased the number of adherent leukocytes in response to anti-GBM antibody ( Figure 9B).…”

Section: Platelet Activation Is Required For Anti-gbm Antibody-inducementioning

confidence: 97%

“…37 Other inhibitory molecules used were as follows: goat polyclonal antibody against mouse fibrinogen (Nordic Immunology, Tiburg, Netherlands; 4 mg/kg) 20 ; YN1/1.7.4, a rat monoclonal antibody against murine ICAM-1 (grown from hybridoma; 200 g/ mouse); RB6-8C5, a monoclonal antibody against Gr-1 (grown from hybridoma; 150 g/mouse); and apyrase, an enzyme that metabolizes adenosine diphosphate (ADP), thereby limiting platelet activation (Sigma-Aldrich, Castle Hill, NSW, Australia; 0.2 U/g). 38 For control experiments, isotype antibodies used included rat IgG 2a and IgG 2b (BD Biosciences, San Diego, CA).…”

Section: Antibodies and Reagentsmentioning

confidence: 99%

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Platelet Recruitment to the Inflamed Glomerulus Occurs via an αIIbβ3/GPVI-Dependent Pathway

Devi

Kuligowski

Kwan

et al. 2010

The American Journal of Pathology

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Recruitment of leukocytes to glomeruli is fundamental to the pathogenesis of many forms of glomerulonephritis. In a model of glomerulonephritis induced by in situ immune complex deposition, we previously observed that, in addition to leukocytes, platelets accumulate in glomerular capillaries, where they contribute to leukocyte recruitment. However, the mechanisms of platelet recruitment and the role of platelet-expressed P-selectin in leukocyte recruitment require further investigation. We used intravital microscopy to examine the mechanisms of platelet and leukocyte recruitment to glomeruli of mice following administration of an antibody against the glomerular basement membrane (anti-GBM antibody). Platelet recruitment was initiated within five minutes of administration of anti-GBM antibody. This was unaltered by inhibition of platelet GPIb␣ but was prevented by the absence of platelet GPVI. Fibrinogen was deposited in glomerular capillaries via a partially intercellular adhesion molecule 1 (ICAM-1)-dependent mechanism, and inhibition of ␣ IIb ␤ 3 , fibrinogen and ICAM-1 inhibited platelet recruitment. Notably, neutrophil depletion also reduced platelet accumulation, indicating a cooperative interaction underlying recruitment of platelets and neutrophils. Finally, using bone marrow chimeras to restrict expression of P-selectin to platelets or endothelial cells, platelet but not endothelial P-selectin was required for glomerular leukocyte recruitment. Together these data indicate that platelet recruitment in this model is dependent on the combined actions of GPVI and the ␣ IIb ␤ 3 /fibrinogen/ ICAM-1 pathway and that platelet P-selectin is crucial for subsequent leukocyte recruitment. (Am J Pathol

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Section: Platelet Activation Is Required For Anti-gbm Antibody-inducementioning

confidence: 97%

Section: Antibodies and Reagentsmentioning

confidence: 99%

Platelet Recruitment to the Inflamed Glomerulus Occurs via an αIIbβ3/GPVI-Dependent Pathway

Devi

Kuligowski

Kwan

et al. 2010

The American Journal of Pathology

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“…With regard to mosquito sialomes, this type of enzyme was previously found solely in Culex quinquefasciatus, and the recombinant enzyme characterized . It is to be noted that transcripts coding for members of the 5′-nucleotidase family, associated with salivary apyrase in mosquitoes (Champagne et al 1995, Sun et al 2006, have not been identified in O. triseriatus, although it was abundantly expressed in Aedes aegypti and Aedes albopictus transcriptomes, and the nucleotidase activity previously reported for O. triseriatus salivary gland homogenates was similar to that of Ae. aegypti in amounts and kinetic characterisitics (pH and divalent cation dependence) (Reno and Novak 2005).…”

Section: Proteins Belonging To Ubiquitous Protein Familiesmentioning

confidence: 82%

The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito,Ochlerotatus triseriatus

et al. 2010

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Saliva of blood-sucking arthropods contains a complex mixture of peptides that affect their host's hemostasis, inflammation, and immunity. These activities can also modify the site of pathogen delivery and increase disease transmission. Saliva also induces hosts to mount an antisaliva immune response that can lead to skin allergies or even anaphylaxis. Accordingly, knowledge of the salivary repertoire, or sialome, of a mosquito is useful to provide a knowledge platform to mine for novel pharmacological activities, to develop novel vaccine targets for vector-borne diseases, and to develop epidemiological markers of vector exposure and candidate desensitization vaccines. The mosquito Ochlerotatus triseriatus is a vector of La Crosse virus and produces allergy in humans. In this work, a total of 1,575 clones randomly selected from an adult female O. triseriatus salivary gland cDNA library was sequenced and used to assemble a database that yielded 731 clusters of related sequences, 560 of which were singletons. Primer extension experiments were performed in selected clones to further extend sequence coverage, allowing for the identification of 159 protein sequences, 66 of which code for putative secreted proteins. Supplemental spreadsheets containing these data are available at http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S1/Ot-S1.xls and http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S2/Ot-S2.xls.

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“…Uno de los parámetros experimentales que afecta el inmunoensayo es la temperatura de incubación de Ag-Ac. Diversas temperatura de incubación han sido reportadas en la literatura, variando de 25 a 37 o C (Sun et al 2006;Santandreu et al,1997). Como es bien sabido, la temperatura óptima de la inmunoreacción es 37 o C. Las proteínas exhiben una mejor actividad para el rango de temperatura entre 20 a 25 o C. A temperaturas superiores puede ocurrir un decaimiento de su actividad y especificidad.…”

Section: Optimización De Las Condiciones Del Análisisunclassified

Construcción de un Inmunosensor Amperométrico utilizando Apirasa de Solanum tuberosum para la Detección de Esquistosomiasis

et al. 2009

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ResumenEste trabajo presenta el desarrollo de un inmunosensor amperométrico composito a base de una proteína de origen vegetal para la determinación de antí-anticuerpo de S. mansoni. El inmunosensor consiste en una matriz electródica rígida construida con grafito y resina epoxi en la que se inmoviliza covalentemente la Apirasa. La estrategia de inmovilización se basa en silanización de la matriz compósita con 3-aminopropilsilano (3-APTES) y su activación mediante glutaraldehído. La inmovilización fue monitoreada mediante voltaamperometría cíclica en Fe(CN) 6 4-/Fe(CN) 6 3 , obteniéndose una respuesta cuasi-reversible del inmunosensor y muy estable en el tiempo. La inmovilización también fue caracterizada mediante ángulos de contactos y microscopía electrónica de barrido. Estas cualidades son aprovechables para la determinación clínica de esquistosomiasis dada la rapidez y sensibilidad de respuesta. Palabras clave: proteína vegetal, inmunosensor amperométrico, inmovilización covalente, compósito Construction of an Amperometric Immunosensor using Solanum tuberosum potato Apyrase for the Detection of Schistosomiasis AbstractThis paper presents the development of an amperometric composites inmunosensor based on a vegetable protein for the determination of anti-Ac S. mansoni. The inmunosensor consists of a rigid matrix built with graphite and epoxy resin in which immobilizes covalently the Apyrase. The strategy of immobilization is based in silanization of the matrix composites with 3-Aminopropyltriethoxysilane (3-APTES) and its activation by glutaraldehyde. The detection was monitored by cyclic voltammetry Fe(CN) 6 4-/ Fe(CN) 6 3 , obtaining a response quasi-reversible of the immunosensor and very stable over time. The immobilization was also characterized by angles contacts and scanning electron microscopy. These qualities are useful for determination clinical of schistosomiasis given the speed of response and sensitivity.

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Expression of functional recombinant mosquito salivary apyrase: A potential therapeutic platelet aggregation inhibitor

Cited by 53 publications

References 38 publications

Platelet Recruitment to the Inflamed Glomerulus Occurs via an αIIbβ3/GPVI-Dependent Pathway

Platelet Recruitment to the Inflamed Glomerulus Occurs via an αIIbβ3/GPVI-Dependent Pathway

The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito,Ochlerotatus triseriatus

Construcción de un Inmunosensor Amperométrico utilizando Apirasa de Solanum tuberosum para la Detección de Esquistosomiasis

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