Expression of Functional P2-Purinergic Receptors in Primary Cultures of Human Colorectal Carcinoma Cells

Extracellular nucleotides inhibit growth of human oesophageal cancer cells via P2Y2-receptors

“…Extracellular ATP is known to inhibit growth of Ehrlich ascites tumour cells, colon cancer, ovarian cancer, endometrial cancer, breast cancer, and fibroblast cell lines (Estrela et al, 1995;Correale et al, 1997;Höpfner et al, 1998;Katzur et al, 1999;Li et al, 2000;Schultze-Mosgau et al, 2000). Here we provide evidence that extracellular nucleotides induce apoptosis and cause cell cycle arrest in human squamous cancer cells of the oesophagus.…”

“…While antiproliferative effects of metabotropic P2Y-receptors have been documented in various tumour models (Fang et al, 1992;Vandewalle et al, 1994;Höpfner et al, 1998;Katzur et al, 1999;Schultze-Mosgau et al, 2000), apoptosis inducing effects of P2Y-receptors have only been shown in MCF-7-breast cancer (Vandewalle et al, 1994) and colorectal carcinoma cells However, the main P2-receptor subtypes implicated in apoptosis are ionotropic P2X-receptors of the subtypes P2X 1 and P2X 7 (Chvatchko et al, 1996;Di Virgilio et al, 1998;Ferrari et al, 1999). P2X-receptors are mainly found in excitable tissues such as smooth muscles and nerves, although P2X 4 -receptors have also been reported in endocrine tissues (Ralevic and Burnstock, 1998).…”

“…Fluorescence measurements were performed at room temperature with a digital imaging system of T.I.L.L. Photonics (München, Germany) Höpfner et al, 1998). Fura-2 fluorescence was excited alternatively at 340 and 380 nm with a monochromator provided by a 75 W xenon lamp.…”

Raloxifene Induces Autophagy-Dependent Cell Death in Breast Cancer Cells via the Activation of AMP-Activated Protein Kinase

“…Csernoch, 2008). The anti-cancer activity of ATP has been reported by many groups, which have reported that ATP-activated P2 receptors decrease tumor growth and increase apoptosis in diverse types of cancers (Hopfner et al, 1998;Wang et al, 2004;White and Burnstock, 2006). Conversely, extracellular ATP activates P2 receptors followed by increases intracellular calcium and cell proliferation of MCF-7 cells, which is supported by ATP acting as a promoter of cell proliferation and growth (Dixon et al, 1997;Wagstaff et al, 2000).…”

Pathophysiology and Therapeutic Potential of Purinergic Signaling

“…Phase 1 clinical trials have shown that ATP infusion in patients with advanced cancer is feasible, but it is limited by chest tightness and dyspnea, probably due to conversion to adenosine (Agteresch et al, 1999). P2Y 2 receptors are expressed in human colon carcinoma cells (Höpfner et al, 1998;Nylund et al, 2004).…”