Expression of functional interleukin 2 receptors by peripheral blood monocytes from patients with active pulmonary tuberculosis.

Toossi, Zahra; Sedor, John R.; Lapurga, John P.; Ondash, Robert J.; Ellner, Jerrold J.

doi:10.1172/jci114635

Cited by 57 publications

(39 citation statements)

References 36 publications

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“…For CD25, we demonstrated that cell-surface and soluble CD25 induced by infection can function as an IL-2 scavenger receptor. This is in line with clinical evidence linking increased levels of sCD25 to immunosuppression in infectious (43)(44)(45) and malignant diseases (46,47 (26).…”

Section: Discussionsupporting

confidence: 88%

Infection of Myeloid Dendritic Cells withListeria monocytogenesLeads to the Suppression of T Cell Function by Multiple Inhibitory Mechanisms

Попов

Driesen

Abdullah

et al. 2008

The Journal of Immunology

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Myeloid dendritic cells (DC) and macrophages play an important role in pathogen sensing and antimicrobial defense. In this study we provide evidence that myeloid DC respond to infection with Listeria monocytogenes with simultaneous induction of multiple stimulatory and inhibitory molecules. However, the overall impact of infected DC during T cell encounter results in suppression of T cell activation, indicating that inhibitory pathways functionally predominate. Inhibitory activity of infected DC is effected mainly by IL-10 and cyclooxygenase 2-mediated mechanisms, with soluble CD25 acting as an IL-2 scavenger as well as by the products of tryptophan catabolism. These inhibitory pathways are strictly TNF-dependent. In addition to direct infection, DC bearing this regulatory phenotype can be induced in vitro by a combination of signals including TNF, TLR2, and prostaglandin receptor ligation and by supernatants derived from the infected cells. Both infection-associated DC and other in vitro-induced regulatory DC are characterized by increased resistance to infection and enhanced bactericidal activity. Furthermore, myeloid DC expressing multiple regulatory molecules are identified in vivo in granuloma during listeriosis and tuberculosis. Based on the in vivo findings and the study of in vitro models, we propose that in granulomatous infections regulatory DC may possess dual function evolved to protect the host from disseminating infection via inhibition of granuloma destruction by T cells and control of pathogen spreading.

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Section: Discussionsupporting

confidence: 88%

Infection of Myeloid Dendritic Cells withListeria monocytogenesLeads to the Suppression of T Cell Function by Multiple Inhibitory Mechanisms

Попов

Driesen

Abdullah

et al. 2008

The Journal of Immunology

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“…Because IL-2R was detected predominantly on T-lymphocytes after stimulation by purified protein derivative (PPD) [17], we have reason to believe that T-cells are the major source of sIL-2R in patients with active TB. However, an in vitro study has demonstrated the expression of IL-2R by peripheral blood monocytes from patients with active pulmonary TB [18]. Therefore, monocytes may contribute to the pool of circulating sIL-2R in patients with active TB.…”

Section: Discussionmentioning

confidence: 99%

Elevated interleukin-2 receptor level in patients with active pulmonary tuberculosis and the changes following anti-tuberculosis chemotherapy

Chan¹,

Lai²,

Leung³

et al. 1995

Eur Respir J

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E El le ev va at te ed d i in nt te er rl le eu uk ki in n--2 2 r re ec ce ep pt to or r l le ev ve el l i in n p pa at ti ie en nt ts s w wi it th h a ac ct ti iv ve e p pu ul lm mo on na ar ry y t tu ub be er rc cu ul lo os si is s a an nd d t th he e c ch ha an ng ge es s f fo ol ll lo ow wi in ng g a an nt ti i--t tu ub be er rc cu ul lo os si is s c ch he em mo ot th he er ra ap py y Forty four patients with pulmonary TB or tuberculous pleural effusion were recruited. Serum was collected from the patients before and at 1, 2, 4 and 6 months after initiation of anti-TB chemotherapy. Serum sIL-2R level was measured by an enzyme immunoassay. The mean sIL-2R level before treatment was 1,452±103 (SEM) U·ml -1 , which was significantly higher than that of healthy control subjects (374±30 U·ml -1 ). There was no significant change in the sIL-2R level at 1 month, but there was a gradual reduction from the second month onwards. At the sixth month the mean sIL-2R level was 1080±81 U·ml -1 , which was significantly lower than that before treatment. However, despite clinical improvement, the sIL-2R levels at the sixth month were still significantly higher than those of control subjects.We conclude that sIL-2R levels were elevated in patients with pulmonary TB and there was a gradual reduction following anti-TB chemotherapy. However, the sIL-2R levels were still higher than control subjects at completion of treatment, suggesting a delayed resolution of the inflammation in patients with pulmonary TB.

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“…The demonstration of M. tuberculosis in the peripheral blood of animals, demonstrated after inoculation and culture [23], led us to investigate the suitability of patients' blood for detection of M. tuberculosis by PCR. Moreover, the possibility of mycobacteraemia has been suggested recently as activation of circulating human peripheral blood monocytes, which results in the expression of gene for TL-2R followed in turn by the appearence of IL-2R on the cells surface [24,25] and the increase of IL-1 production, has been demonstrated during active human tuberculosis [26]. On the basis of these considerations this new technique was applied to the detection of M. tuberculosis in peripheral blood of patients with acute tuberculosis.…”

Section: Discussionmentioning

confidence: 99%

Detection of Mycobacterium Tuberculosis DNA in Blood of Patients with Acute Pulmonary Tuberculosis by Polymerase Chain Reaction and Non-Isotopic Hybridisation Assay

Prete

D'Alagni²,

Sabato³

et al. 1997

Journal of Medical Microbiology

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The detection of Mycobacterium tuberculosis DNA in peripheral blood mononuclear cells (PBMC) by PCR and non-isotopic hybridisation assay was evaluated for the laboratory diagnosis of pulmonary M. tuberculosis infection. The PCR technique was based on the presence of IS6120, a DNA sequence specific for M. tuberculosis, and performed on PBMC from 30 patients belonging to the fifth group of the American Thoracic Society (ATS) classification of tuberculosis. The identification of amplification products was confirmed after electrophoresis by hybridisation with a non-isotopic probe in a DNA enzyme immunoassay (DEIA). Of the 30 blood samples studied by the PCR-DEIA technique, 26 gave positive results and four gave negative results. Blood samples from 30 subjects in a control group were negative by this technique. The data suggest that PCR-DEIA of blood may provide a sensitive, specific and useful means of diagnosing mycobacterial infection.

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Expression of functional interleukin 2 receptors by peripheral blood monocytes from patients with active pulmonary tuberculosis.

Abstract: Peripheral blood monocytes from patients with active

Cited by 57 publications

References 36 publications

Infection of Myeloid Dendritic Cells withListeria monocytogenesLeads to the Suppression of T Cell Function by Multiple Inhibitory Mechanisms

Infection of Myeloid Dendritic Cells withListeria monocytogenesLeads to the Suppression of T Cell Function by Multiple Inhibitory Mechanisms

Elevated interleukin-2 receptor level in patients with active pulmonary tuberculosis and the changes following anti-tuberculosis chemotherapy

Detection of Mycobacterium Tuberculosis DNA in Blood of Patients with Acute Pulmonary Tuberculosis by Polymerase Chain Reaction and Non-Isotopic Hybridisation Assay

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