Expression of functional follicle-stimulating hormone receptor and luteinizing hormone/chorionic gonadotrophin receptor in oviduct and uterus in prepubertal gilts

Liang, Hongyan; Lu, Chao; Liu, Zhuo; Zhou, Xu

doi:10.1016/j.livsci.2012.05.012

Cited by 8 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This shift was characterized by the transient increase in the antral follicles growth and in the plasma LH levels, and the establishment of the fully development of the kisspeptin neuronal system in hypothalamus. Accordingly, gonadotropin receptors were reported in the cervix of the ewe (Leethongdee, Khalid, & Scaramuzzi, ; Rodríguez‐Piñón et al, ) and its presence in uterus of gilts was related to the uterine prepubertal development (Liang, Chen, Liu, & Zhou, ). Moreover, gonadotrophin hormones induced the remodelling of cervical ECM in the ewe (Leethongdee, Khalid, & Scaramuzzi, ) and were able to regulate the expression of MMPs in cultured human granulosa cells (Aston et al, ), suggesting that the later increase in L MMP‐2 levels of activity could be consequence of the gonadotropin effect on MMP‐2 expression.…”

Section: Discussionmentioning

confidence: 99%

Collagen, glycosaminoglycans and matrix metalloproteinase‐2 and metalloproteinase‐9 in the cervix of the ewe during prepubertal development

Rodrı́guez-Piñón

Casuriaga

Genovese

et al. 2019

Anat Histol Embryol

View full text Add to dashboard Cite

The tortuous nature of the ovine cervix restricts the transcervical passage of the cannula, and many studies have aimed to understand the endocrine mechanism of the remodelling of cervical tissue in adult ewe. However, little is known about the remodelling of the cervical tissue during the prepubertal development of the lambs. To obtain histochemical and biochemical evidence about the nature of the prepubertal development of the cervix of the ewe, cervices of Corriedale lambs obtained at 0, 1, 2, 4, 6 and 8 months of age (n = 5 to 6 in each) were processed. Neutral and acidic glycosaminoglycans (by PAS‐Alcian stain) were weakly in the cervical stroma and not shown change during the development, whereas the percentage volume of fibrillar collagen (by van Gieson stain) increases throughout the experimental period in the superficial fold stroma and deep wall stroma (p < 0.05). The relative cervical weight (g/kg of body weight) and the collagen concentration (by spectrophotometry, mg/mg wet tissue) showed an early decreasing phase from months 0 to 4 and a later increasing phase from months 4 to 8 (p < 0.05). The latent form of matrix metalloproteinase‐2 (MMP‐2) detected by gelatin zymography (ng/mg protein) decreased from months 0 to 2 and increased from months 4 to 8, whereas the activated form decreased from months 0 to 2, remained low until month 6 and then recovered on month 8 (p < 0.0001). Data suggest that the relative cervical weight biphasic pattern during the development is related to MMP‐2‐dependent changes in the collagen content.

show abstract

Section: Discussionmentioning

confidence: 99%

Collagen, glycosaminoglycans and matrix metalloproteinase‐2 and metalloproteinase‐9 in the cervix of the ewe during prepubertal development

Rodrı́guez-Piñón

Casuriaga

Genovese

et al. 2019

Anat Histol Embryol

View full text Add to dashboard Cite

show abstract

“…An increased expression of FSHR at proestrus and oestrus in the Yak uterus as observed in our study may therefore be potentially involved in increased angiogenesis in the uterus, thus preparing it for implantation, in case pregnancy happens. In porcine uterus vascular smooth muscle cells, FSHR expression was significantly increased during the luteal phase (Liang et al, ). Our study showed no FSHR‐positive staining in vascular smooth muscle cells, and only a weak expression of FSHR in vascular endothelial cells was observed.…”

Section: Discussionmentioning

confidence: 99%

“…Follicle‐stimulating hormone receptor (FSHR) is also expressed in osteoclasts where FSH signalling mediates bone resorption (Zhu et al, ), and in neuroendocrine tumours (NETs) where its role has not yet been elucidated (Pawlikowski, Winczyk, & Stępień, ). Recently, LiangChen, Liu, and Zhou () demonstrated that FSHR expressed in the porcine uterus with differences according to the stage of the oestrous cycle. Follicle‐stimulating hormone receptor (FSHR) has been reported expressed in the uterus of other species including human (Kornyei, Li, Lei, & Rao, ), porcine (Liang et al, ) and mice (Wei, Guo, Gong, Zhang, & Ma, ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, LiangChen, Liu, and Zhou () demonstrated that FSHR expressed in the porcine uterus with differences according to the stage of the oestrous cycle. Follicle‐stimulating hormone receptor (FSHR) has been reported expressed in the uterus of other species including human (Kornyei, Li, Lei, & Rao, ), porcine (Liang et al, ) and mice (Wei, Guo, Gong, Zhang, & Ma, ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression and localization of follicle‐stimulating hormone receptor in the yak uterus during different stages of the oestrous cycle

Abudureyimu

Cai

Huo

et al. 2018

Reprod Domestic Animals

View full text Add to dashboard Cite

Morphological changes of the uterus and alterations in its secretory activity under the influence of steroid hormones been well documented. The oestrous cycle is also associated with significant changes in plasma follicle-stimulating hormone (FSH), whose effects are mediated through its receptor (FSHR). Reports showed that in many mammals, FSHR was expressed in gonadal and extragonadal tissues including cervix, female reproductive tract, and pituitary gland. Follicle-stimulating hormone (FSH) signals through endothelial FSHR directly stimulate angiogenesis and involved in the timing of birth in human, and the presence of FSHR in the placenta is essential for normal pregnancy in mice. But little is known about FSHR expression in the yak uterus. The main objective of the present study was to determine the expression and localization of FSHR in the yak uterus during different phases of the oestrous cycle. Results showed that FSHR protein was localized in the surface and glandular epithelial cells, stroma cells, myometrial smooth muscle cells and blood vessel endothelial cells. The expression of FSHR protein peaked at oestrus, significantly decreased at dioestrus (p < 0.05) and increased again at proestrus. FSHR mRNA was highly expressed at both proestrus and oestrus, and decreased at metestrus with the lowest values at dioestrus (p < 0.05). In conclusion, FSHR expression in the yak uterus changed with the stage of the oestrous cycle suggesting that FSHR plays an essential role in regulating the endometrial and myometrial functions during the oestrus cycle in the yak.

show abstract

“…These receptors are primarily expressed on the surface of gonadal somatic cells and are involved in the regulation of gametogenesis via distinct intracellular signaling pathways (Menon and Menon 2012). The gonadotropin receptors (GtHRs) molecules have already been cloned, and their physiological roles in mammals, chicken, and some teleost fish have been clarified (Johnson et al 1996;Akazome et al 2002;Mittelholzer et al 2009;Liang et al 2012;Ohkubo et al 2013). Two classes of GtHRs namely type I (GtH-RI) and type II (GtH-RII) are found in fish gonads, and they correspond to the FSHR and LHR in mammals and chicken (Oba et al 2001;Levavi-Sivan et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot (Scophthalmus maximus)

Jia

Meng

Niu

et al. 2014

Fish Physiol Biochem

View full text Add to dashboard Cite

The luteinizing hormone receptor (LHR) plays a crucial role in female reproduction. In the present study, full-length sequence coding for the LHR was obtained from female turbot (Scophthalmus maximus) by homology cloning and a strategy based on rapid amplification of cDNA end-polymerase chain reaction. The full-length LHR cDNA was 3,184 bp long and contained a 2,058-bp open reading frame which encoded a protein of 685 amino acids. Multiple sequence alignments of the turbot LHR manifested high homologies with the corresponding sequences of available teleosts and representative vertebrates, and significant homology with that of Hippoglossus hippoglossus. In addition, the turbot LHR showed typical characteristics of glycoprotein receptors, including a long N-terminal extracellular domain, seven transmembrane domains, and a short C-terminal intracellular domain. LHR mRNA was abundant in the ovary, but was deficient in extra-ovarian tissues. Furthermore, LHR mRNA gradually developed from previtellogenesis to migratory nucleus stage, with the highest values observed in migratory nucleus stage during reproductive cycle. However, LHR mRNA sharply decreased in atresia stage. These results suggested that LHR is a typical G protein-coupled receptor that is involved in the promotion of turbot ovarian development and may be related to the final maturation and ovulation of oocyte. These findings contribute to the understanding of the potential roles of LHR in controlling the fish reproductive cycle.

show abstract

Expression of functional follicle-stimulating hormone receptor and luteinizing hormone/chorionic gonadotrophin receptor in oviduct and uterus in prepubertal gilts

Cited by 8 publications

References 17 publications

Collagen, glycosaminoglycans and matrix metalloproteinase‐2 and metalloproteinase‐9 in the cervix of the ewe during prepubertal development

Collagen, glycosaminoglycans and matrix metalloproteinase‐2 and metalloproteinase‐9 in the cervix of the ewe during prepubertal development

Expression and localization of follicle‐stimulating hormone receptor in the yak uterus during different stages of the oestrous cycle

Molecular cloning, characterization, and expression analysis of luteinizing hormone receptor gene in turbot (Scophthalmus maximus)

Contact Info

Product

Resources

About