Expression of fructose 1,6-bisphosphatase and phosphofructokinase is induced in hepatopancreas of the white shrimp Litopenaeus vannamei by hypoxia

Cota-Ruíz, Keni; Peregrino‐Uriarte, Alma B.; Félix-Portillo, Monserrath; Martínez-Quintana, José A.; Yépiz-Plascencia, Glória

doi:10.1016/j.marenvres.2015.02.003

Cited by 64 publications

(37 citation statements)

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“…An increase in the expression of the genes encoding the α and β subunits of the HIF‐1 was also observed; the direct relationship between the expression of genes from anaerobic glycolysis (PFK, LDH1, LDH2) and subunits of HIF‐1 (α, β) support its presumed function as regulator in the activation of genes to maintain the cellular energy state during hypoxia. A similar response has been observed in the expression of the genes PFK and fructose 1,6‐bisphosphatase (FBP) in hepatopancreas and LDH and hexokinase in gills, in response to hypoxia which has been attributed to the activation of genes related to anaerobic metabolism during hypoxic stress via HIF‐1 (Cota‐Ruiz et al, ; Soñanez‐Organis, Peregrino‐Uriarte, Sotelo‐Mundo, Forman, & Yepiz‐Plascencia, ; Soñanez‐Organis et al, ).…”

Section: Discussionsupporting

confidence: 54%

“…Most of the studies focus on the molecular response mediated by the hypoxia inducible factor (HIF), a heterodimeric transcription factor that activates or deactivates genes related to the synthesis of oxygen transporters, GLU transporters and glycolytic pathways (Schito & Rey, 2017;Semenza, 2012; Soñanez-Organis, Racotta, & Yepiz-Plascencia, 2010). In addition, the induction of gene expression or increase in enzyme activity of glycolytic enzymes in shrimp was demonstrated during exposure to hypoxia, for example, phosphofructokinase (PFK), an enzyme that catalyses the non-reciprocal inter-conversion between fructose 6-phosphate and fructose 1,6-bisphosphate and a key regulatory enzyme of glycolysis is induced during hypoxia supporting the assumption that glycolysis rate rises in hepatopancreatic shrimp cells (Cota-Ruiz et al, 2016;Cota-Ruiz, Peregrino-Uriarte, Felix-Portillo, Martínez-Quintana, & Yepiz-Plascencia, 2015; Sánchez-Paz, Soñanez-Organis, Peregrino-Uriarte, Muhlia-Almazán, & Yepiz-Plascencia, 2008). On the other hand, LAC dehydrogenase (LDH) transcript concentration is considered as a marker to analyse the transition of aerobic to anaerobic glycolysis during hypoxia since it is well known that this gene is induced and regulated in gills through the action of hypoxia inducible factor 1 (HIF-1) in L. vannamei (Soñanez-Organis et al, 2012).…”

Section: Introductionmentioning

confidence: 80%

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Gene expression and energetic metabolism changes in the whiteleg shrimp (Litopenaeus vannamei) in response to short‐term hypoxia

Ulaje¹,

Rojo‐Arreola²,

Lluch‐Cota³

et al. 2019

Aquac Res

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In shrimp, several physiological, metabolic and gene expression patterns related to the use of carbohydrates have been associated with hypoxia since this limiting factor modifies aerobic glycolysis to anaerobic glycolysis. The aim of this work was to analyse the effect of short‐term hypoxia on oxygen consumption (OC), carbohydrate content and expression of hypoxia inducible factor 1 (HIF‐1), glycolysis and Krebs’ cycle genes in Litopenaeus vannamei juvenile shrimps to elucidate use of energy in shrimps during the periods of low oxygen concentration commonly found in culture ponds. At low oxygen concentration, a decrease in shrimp OC, an increase in carbohydrate content and an increase in expression of HIF‐1, glycolysis and Krebs’ cycle genes in shrimp gills were registered. These results might indicate that exposure of L. vannamei to short‐term hypoxia implies the use of carbohydrates as metabolic fuel triggered by an increase in transcription of HIF‐1, glycolysis and Krebs’ cycle pathway genes, as a strategy to maintain a balance between oxidative and anaerobic metabolism and to sustain the energy supply as a compensation to low oxygen availability.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 80%

Gene expression and energetic metabolism changes in the whiteleg shrimp (Litopenaeus vannamei) in response to short‐term hypoxia

Ulaje¹,

Rojo‐Arreola²,

Lluch‐Cota³

et al. 2019

Aquac Res

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“…MnHK mRNA was constitutively expressed in all the examined tissues, with higher expression in the muscle and hepatopancreas (Figure 5), which was similar to previous studies in chicken [21], mouse [22], and fish [23]. Hepatopancreas functions include the production of digestive enzymes and hemolymph proteins, and the absorption of nutrients [24], while anaerobic respiration mainly occurs in muscles with functions in locomotion and gluconeogenesis [25]. It is reasonable to think that glycolysis would occur in certain cells of the hepatopancreas and muscle, which would explain the high level of MnHK transcripts in the hepatopancreas and muscle.…”

Section: Resultssupporting

confidence: 85%

Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia

Sun

Xuan

et al. 2017

IJMS

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Metabolic adjustment to hypoxia in Macrobrachium nipponense (oriental river prawn) implies a shift to anaerobic metabolism. Hexokinase (HK) is a key glycolytic enzyme in prawns. The involvement of HK in the hypoxia inducible factors (HIFs) pathway is unclear in prawns. In this study, the full-length cDNA for HK (MnHK) was obtained from M. nipponense, and its properties were characterized. The full-length cDNA (2385 bp) with an open reading frame of 1350 bp, encoded a 450-amino acid protein. MnHK contained highly conserved amino acids in the glucose, glucose-6-phosphate, ATP, and Mg+2 binding sites. Quantitative real-time reverse transcription PCR assays revealed the tissue-specific expression pattern of MnHK, with abundant expression in the muscle, and gills. Kinetic studies validated the hexokinase activity of recombinant HK. Silencing of HIF-1α or HIF-1β subunit genes blocked the induction of HK and its enzyme activities during hypoxia in muscles. The results suggested that MnHK is a key factor that increases the anaerobic rate, and is probably involved in the HIF-1 pathway related to highly active metabolism during hypoxia.

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“…Carbohydrate metabolism generates the ATP necessary for Na + /K + ‐ATPase to function, and in this way, PFK could be a key factor in accelerating glycolysis to produce more ATP (Cota‐Ruiz et al . ). In our study, the 300 mg L −1 group shrimps showed higher levels of HK and PFK mRNA, indicating that these shrimps had a higher capacity to produce ATP.…”

Section: Discussionmentioning

confidence: 97%

A diet of fructose-enrichedArtemiaimproves the response of juvenileLitopenaeus vannameishrimp to acute low-salinity challenge

et al. 2016

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The Pacific white shrimp (Litopenaeus vannamei), as an economically important species, has been reared in low‐salinity water during the last decade. To investigate how juvenile L. vannamei shrimp fed with fructose‐enriched Artemia respond to acute low‐salinity stress, the shrimp were randomly divided into four treatment groups, three groups were fed with Artemia enriched with either 100, 200 and 300 mg L−1 of fructose and a control group fed with Artemia with no enrichment for 10 days. The results showed that the 300 mg L−1 fructose group demonstrated the maximum survival rate and glycogen content. Additionally, the 300 mg L−1 fructose group showed significantly higher Na+/K+‐ATPase activity, total antioxidant capacity, and expression levels of Na+/K+‐ATPase α‐subunit, V‐H ATPase α‐subunit, hexokinase, phosphofructokinase, antioxidants (SOD, CAT, GPX) and Hsp70 mRNA when compared with the control group. Furthermore, after exposure to low salinity, the mRNA levels of phosphofructokinase, V‐H ATPase α‐subunit, GPX, p38, JNK and Rac1 stayed constant in shrimp fed with fructose‐enriched Artemia but changed significantly in the control group. Thus, a diet of fructose‐enriched Artemia can improve the osmoregulation and survival of juvenile L. vannamei shrimp exposed to low salinities.

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Expression of fructose 1,6-bisphosphatase and phosphofructokinase is induced in hepatopancreas of the white shrimp Litopenaeus vannamei by hypoxia

Cited by 64 publications

References 50 publications

Gene expression and energetic metabolism changes in the whiteleg shrimp (Litopenaeus vannamei) in response to short‐term hypoxia

Gene expression and energetic metabolism changes in the whiteleg shrimp (Litopenaeus vannamei) in response to short‐term hypoxia

Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia

A diet of fructose-enrichedArtemiaimproves the response of juvenileLitopenaeus vannameishrimp to acute low-salinity challenge

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