Expression of FoxP3, a key molecule in CD4⁺CD25⁺ regulatory T cells, in adult T‐cell leukaemia/lymphoma cells

Abstract

SummaryAdult T-cell leukaemia/lymphoma (ATLL) is an aggressive neoplastic disease that usually exhibits a CD4 + CD25 + phenotype. Regulatory T cells (Treg), which suppress T-cell effector function, are characterized by the co-expression of CD4 and CD25. We analysed the expression of forkhead/ winged helix transcription factor (FoxP3), a specific marker that is important for the function of Treg, on ATLL cells from 17 patients (peripheral blood, n ¼ 8; lymph node, n ¼ 9). Real-time polymerase chain reaction and immunostaining detected FoxP3 expression in 10 ATLL cases, but was relatively down-regulated compared with Treg from normal subjects. These results indicate the association of ATLL and Treg.

Clinical significance of CADM1/TSLC1/IgSF4 expression in adult T-cell leukemia/lymphoma

“…5,6 Initially, the CD4 þ CD25 þ cell fraction was separated from PBMCs of a healthy volunteer by the magnetic bead method and stained with an anti-CADM1 antibody. Almost 100% of the S1T-ATLL cell line was strongly stained with the anti-CADM1 antibody; however, 55.8% of the CD4 þ CD25 þ cells were stained weakly in comparison with the high level of staining of S1T-ATLL cells (Figure 2a).…”

“…2 A fraction of ATLL cases have been shown to also express forkhead box P3 (FOXP3), which is a master gene for regulatory T cells (T-reg), suggesting that some cases of ATLL may originate from HTLV-1-infected T-reg cells. 5,6 For diagnosis, identification of mono-or oligoclonal provirus integration events by Southern blot analysis is one of the definitive markers for ATLL. In addition to viral integration, ATLL cells with multi-lobulated nuclei (called 'flower cells') have been frequently seen in leukemia cells in the peripheral blood of ATLL patients.…”

Circulating and Liver Resident CD4+CD25+ Regulatory T Cells Actively Influence the Antiviral Immune Response and Disease Progression in Patients with Hepatitis B

“…The probe and primers used for FoxP3 were as follows: FAM-5Ј-CAC AGA TGA AGC CTT GGT CAG TGC CA-3Ј-TAMRA; 5Ј-GAG AAG CTG AGT GCC ATG CA-3Ј; and 5Ј-AGG AGC CCT TGT CGG ATG AT-3Ј. The probe and primers used for GAPDH were as follows: FAM-5Ј-AAG GTG AAG GTC GGA GTC AAC GGA TTT G-3Ј-TAMRA; 5Ј-CCA CAT CGC TCA GAC ACC AT-3Ј; and 5Ј-CCA GGC GCC CAA TAC G-3Ј (41). The one-step RT-PCR kit (Qiagen) was used for RT-PCR amplification.…”

Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia

“…ATL/ATLL cells were initially shown to be positive for mature T-cell surface antigens CD3, CD4, and CD25, and negative for CD8 with rare occasions of double negativity or double positivity for CD4 and CD8 (Hattori et al 1981;Hattori et al 1991). Subsequent studies on the nature of ATL/ATLL cells have shown that they are also positive for regulatory T-cell (Treg) markers, such as FoxP3 (Karube et al 2004;Roncador et al 2005) and the CC chemokine receptor 4 (CCR4) (Yoshie et al 2002). Moreover, several secreted (cytokine) and cell surface-associated (receptor) factors serve as important clues for monitoring the activity and prognosis of patients with ATL/ATLL (Yasuda et al 1988;Yamada et al 1996;Mori and Prager 1998).…”