Expression of four pha genes involved in poly-β-hydroxybutyrate production and accumulation in Rhodobacter sphaeroides FJ1

Chou, Min-En; Chang, Wen-Tuan; Chang, Ya-Chieh; Yang, Ming‐Der

doi:10.1007/s00438-009-0448-4

Cited by 14 publications

(18 citation statements)

References 31 publications

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“…sphaeroides (Wieczorek et al ., ). phaP expression is regulated by its specific regulator, PhaR, during active growth (Chou et al ., ). However, the importance of PHA production for the growth and survival of R .…”

Section: Resultsmentioning

confidence: 97%

Adaptation of the Alphaproteobacterium Rhodobacter sphaeroides to stationary phase

McIntosh

Eisenhardt

Remes

et al. 2019

Environmental Microbiology

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Summary Exhaustion of nutritional resources stimulates bacterial populations to adapt their growth behaviour. General mechanisms are known to facilitate this adaptation by sensing the environmental change and coordinating gene expression. However, the existence of such mechanisms among the Alphaproteobacteria remains unclear. This study focusses on global changes in transcript levels during growth under carbon‐limiting conditions in a model Alphaproteobacterium, Rhodobacter sphaeroides, a metabolically diverse organism capable of multiple modes of growth including aerobic and anaerobic respiration, anaerobic anoxygenic photosynthesis and fermentation. We identified genes that showed changed transcript levels independently of oxygen levels during the adaptation to stationary phase. We selected a subset of these genes and subjected them to mutational analysis, including genes predicted to be involved in manganese uptake, polyhydroxybutyrate production and quorum sensing and an alternative sigma factor. Although these genes have not been previously associated with the adaptation to stationary phase, we found that all were important to varying degrees. We conclude that while R. sphaeroides appears to lack a rpoS‐like master regulator of stationary phase adaptation, this adaptation is nonetheless enabled through the impact of multiple genes, each responding to environmental conditions and contributing to the adaptation to stationary phase.

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Section: Resultsmentioning

confidence: 97%

Adaptation of the Alphaproteobacterium Rhodobacter sphaeroides to stationary phase

McIntosh

Eisenhardt

Remes

et al. 2019

Environmental Microbiology

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“…This could be due to the effects of other molecular mechanisms for PHA degradation. First, PhaR plays a negative role in the regulation of PHA synthesis (12, 28). Excess PhaR can repress the expression of PhaP (Phasin) by binding to upstream DNA regions of phaP and phaR (39).…”

Section: Resultsmentioning

confidence: 99%

Proteomic Responses of <i>Roseobacter litoralis</i> OCh149 to Starvation and Light Regimen

Zong

Jiao

2012

Microb. Environ.

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Roseobacter litoralis OCh149 is a type strain of aerobic anoxygenic phototrophic bacteria in marine Roseobacter clade. Its full genome has been sequenced; however, proteomic research, which will give deeper insights into the environmental stimuli on gene expression networks, has yet to be performed. In the present study, a proteomic approach was employed to analyze the status of R. litoralis OCh149 in carbon starvation during the stationary phase and its responses to a dark/light regimen (12 h:12 h) in both exponential and stationary phases. LC-MS/MS-based analysis of highly abundant proteins under carbon starvation revealed that proteins involved in transport, the transcription/translation process and carbohydrate metabolism were the major functional categories, while poly-β-hydroxyalkanoate (PHA), previously accumulated in cells, was remobilized after stress. Glucose, as the sole carbon source in the defined medium, was broken down by Entner-Doudoroff and reductive pentose phosphate (PP) pathways. Carbohydrate catabolism-related proteins were down-regulated under light regardless of the growth phase, probably due to inhibition of respiration by light. In contrast, responses of amino acid metabolisms to light regimen varied among different proteins during growth phases depending on cellular requirements for proliferation, growth or survival. Fluorescence induction and relaxation measurements suggested that functional absorption cross-sections of the photosynthetic complexes decreased during the dark period and always recovered to about the previous level during the light period. Although the photosynthetic genes in R. litoralis OCh149 are located on the plasmid, these data indicate the regulatory mechanism of photoheterotroph metabolism by both carbon and light availability.

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“…The consensus PhaR‐binding sequence (CTGCN 3−4 GCAG) of R. sphaeroides is also found in the promoter regions of phaZ, phaC , and phaR in addition to that of phaP , and there is evidence to suggest that PhaR regulates all of these genes (Chou et al ., 2009). A close inspection of the PhaR‐binding sequences of these genes revealed a very striking similarity among them.…”

Section: Discussionmentioning

confidence: 99%

“…Although PhaR can bind to the promoter regions of phaP, phaR, phaZ , and phaC , it regulates these genes differently. PhaR represses the expression of phaP, phaR , and phaZ , but not phaC (Chou et al ., 2009). The phaZ and phaC genes are located next to each other in an opposite direction and share the same PhaR‐binding motif.…”

Section: Discussionmentioning

confidence: 99%

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Analyses of binding sequences of the PhaR protein of Rhodobacter sphaeroides FJ1

Chou

Yang

2010

FEMS Microbiology Letters

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The phaC, phaP, phaR, and phaZ genes are involved in the synthesis, accumulation, and degradation of poly-beta-hydroxybutyrate (PHB). These genes encode the PHB synthase, phasin, regulatory protein, and PHB depolymerase, respectively, and are located in the same locus in the genome of Rhodobacter sphaeroides FJ1, a purple nonsulfur bacterium capable of producing PHB. We have previously found that the PhaR protein binds to the promoter regions of phaP, phaR, and phaZ and represses their expression. In this study, we determined that PhaR binds to an 11-bp palindromic sequence, 5'-CTGCN(3)GCAG-3', located at nucleotides -69 to -59 and -97 to -87 relative to the translation start site of phaP. Substitution of the three spacer nucleotides with any three or four nucleotides in this sequence had no effect on PhaR binding, but all other base deletions or substitutions in this sequence abolished its ability to bind PhaR both in vitro and in vivo. These results suggest that PhaR regulates the expression of phaP in R. sphaeroides FJ1.

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Expression of four pha genes involved in poly-β-hydroxybutyrate production and accumulation in Rhodobacter sphaeroides FJ1

Cited by 14 publications

References 31 publications

Adaptation of the Alphaproteobacterium Rhodobacter sphaeroides to stationary phase

Adaptation of the Alphaproteobacterium Rhodobacter sphaeroides to stationary phase

Proteomic Responses of <i>Roseobacter litoralis</i> OCh149 to Starvation and Light Regimen

Analyses of binding sequences of the PhaR protein of Rhodobacter sphaeroides FJ1

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