Expression of foreign epitopes in P-fimbriae ofEscherichia coli

Die, Irma van; Oosterhout, Joost van; Megen, Ingrid van; Bergmans, Hans; Hoekstra, W. P. M.; Enger-Valk, B.E.; Barteling, S. J.; Mooi, Frits R.

doi:10.1007/bf00633832

Cited by 22 publications

(7 citation statements)

References 36 publications

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“…This construction resulted in the insertion of 27 additional amino acid residues into FasA. A third TGEV epitope could not be added to this site (data not shown), suggesting that successful fimbria export and assembly constrain the length of an inserted segment, as proposed previously (4,7,73,76). Moreover, as observed with the CS31A fimbriae (7,46), the addition of the TGEV A epitope resulted both in decreased fimbria production and in significant cleavage of chimeric subunits.…”

Section: Discussionmentioning

confidence: 62%

Mucosal and Systemic Immune Responses to Chimeric Fimbriae Expressed bySalmonella entericaSerovar Typhimurium Vaccine Strains

Chen

Schifferli

2000

Infect Immun

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Recombinant live oral vaccines expressing pathogen-derived antigens offer a unique set of attractive properties. Among these are the simplicity of administration, the capacity to induce mucosal and systemic immunity, and the advantage of permitting genetic manipulation for optimal antigen presentation. In this study, the benefit of having a heterologous antigen expressed on the surface of a live vector rather than intracellularly was evaluated. Accordingly, the immune response of mice immunized with a Salmonella enterica serovar Typhimurium vaccine strain expressing the Escherichia coli 987P fimbrial antigen on its surface (Fas ؉ ) was compared with the expression in the periplasmic compartment (Fas

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Section: Discussionmentioning

confidence: 62%

Mucosal and Systemic Immune Responses to Chimeric Fimbriae Expressed bySalmonella entericaSerovar Typhimurium Vaccine Strains

Chen

Schifferli

2000

Infect Immun

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“…Several bacterial surface proteins have been successfully fused and found to express foreign proteins. These include PhoE [3], LamB [11], peptidoglycan-associated lipoprotein [15], flagellin [24,27], P-fimbriae [18,40] and TraT [37].…”

Section: Discussionmentioning

confidence: 99%

Expression of Foreign Antigens on the Surface of <i>Escherichia coli</i> by Fusion to the Outer Membrane Protein TraT

Chang¹,

Sheu²,

Lo³

1999

J Biomed Sci

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The traT gene is one of the F factor transfer genes and encodes an outer membrane protein which is involved in interactions between an Escherichia coli and its surroundings. This protein was altered so as to permit the expression of foreign proteins on the outer membrane of E. coli in this study. A 729-bp DNA fragment, including the leader and entire structural gene sequence of traT, was amplified and obtained by PCR. This sequence was then subcloned downstream of the tac promoter of pDR540, resulting in a TraT expression vector, pT2. Here, we report that the expression of TraT protein, fused either with a partial pre-S antigen of hepatitis B virus (60 and 98 amino acids, respectively) or with the snake venom rhodostomin (72 amino acids), was successfully achieved on the outer membrane of E. coli, using the pT2 plasmid. This result was demonstrated using dot blot and immunofluorescence analysis. This finding supports the notion that the pT2 plasmid can be used as an E. coli display system. This system can detect a foreign peptide of about 100 amino acid residues in length on the bacterial surface.

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“…Fimbriae are adhesive surface organelles that enable bacteria to target and colonize specific host tissues. A large variety of fimbrial proteins have been used for surface display, including the FimA and FimH proteins of type 1 fimbriae [34,58], the FelA subunit of type P fimbriae [59] and the major structural subunit of type 4 fimbriae, to name a few [60]. Fimbriae proteins are present at extremely high numbers on the cell surface, which make them attractive for display purposes; however, the major structural proteins of various fimbriae can only accommodate relatively small inserts (10–30 amino acids) without disturbing the organelle structure and surface‐ display efficiency [61].…”

Section: Surface‐display Systems For Bacteriamentioning

confidence: 99%

Biotechnological applications for surface‐engineered bacteria

Wernérus

Ståhl

2004

Biotech and App Biochem

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Display of heterologous proteins on the surface of micro-organisms, enabled by means of recombinant DNA technology, has become an increasingly popular strategy in microbiology, biotechnology and vaccinology. Both Gram-negative and Gram-positive bacteria have been investigated for potential applications. The present review will describe the most commonly used systems for bacterial display, with a focus on the biotechnology applications. Live bacterial vaccine-delivery vehicles have long been investigated through the surface display of foreign antigens and, recently, 'second-generation' vaccine-delivery vehicles have been generated by the addition of mucosal targeting signals, as a means to increase immune responses. Engineered bacteria have also the potential to act as novel microbial biocatalysts with heterologous enzymes immobilized as surface exposed on the bacterial cell surface. They provide the potential for new types of whole-cell diagnostic devices, since single-chain antibodies and other type of tailor-made binding proteins can be displayed on bacteria. Bacteria with increased binding capacity for certain metal ions can be created, and potential environmental or biosensor applications for such recombinant bacteria as biosorbents are being explored. Certain bacteria have also been employed to display various polypeptide libraries for use as devices in in vitro selection applications. Part of the present review has been devoted to a more in-depth description of a promising Gram-positive display system, i.e. Staphylococcus carnosus, and its applications. The review describes the basic principles of the different bacterial display systems and discusses current uses and possible future trends of these emerging technologies.

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Expression of foreign epitopes in P-fimbriae ofEscherichia coli

Cited by 22 publications

References 36 publications

Mucosal and Systemic Immune Responses to Chimeric Fimbriae Expressed bySalmonella entericaSerovar Typhimurium Vaccine Strains

Mucosal and Systemic Immune Responses to Chimeric Fimbriae Expressed bySalmonella entericaSerovar Typhimurium Vaccine Strains

Expression of Foreign Antigens on the Surface of <i>Escherichia coli</i> by Fusion to the Outer Membrane Protein TraT

Biotechnological applications for surface‐engineered bacteria

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