Expression of Follicle-Stimulating Hormone Receptor Variants During the Estrous Cycle of the Ewe.

Sullivan, Rachael R.; Eborn, D.R.; Faris, Brian R.; Grieger, David M.; Rozell, Timothy G.

doi:10.1093/biolreprod/81.s1.346

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But FSH-FSH-R3 interaction evidently plays a crucial role during neo-oogenesis and primordial follicle assembly. Sullvian et al [27] have also reported that alternatively spliced Fsh-r3 is the pre-dominant transcript of FSHR in sheep. Babu et al [26] have earlier reported a similar two-fold up-regulation of Fsh-r3 compared to Fsh-r1 after PMSG treatment in mice.…”

Section: Discussionmentioning

confidence: 99%

“…Quantitative RT-PCR studies on sheep granulosa cells collected from follicles in different development stages show that R3 transcript is highly regulated and is 20–50 fold more abundant than R1 in small to medium sized follicles, whereas in pre-ovulatory follicles R3 is 5-fold more expressed than R1 [27]. Interestingly R3 is also more regulated during follicular development after PMSG treatment compared to R1 in mice [26,28].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RETRACTED ARTICLE: Follicle stimulating hormone modulates ovarian stem cells through alternately spliced receptor variant FSH-R3

et al. 2013

View full text Add to dashboard Cite

BackgroundWe have earlier reported that follicle stimulating hormone (FSH) modulates ovarian stem cells which include pluripotent, very small embryonic-like stem cells (VSELs) and their immediate descendants ‘progenitors’ termed ovarian germ stem cells (OGSCs), lodged in adult mammalian ovarian surface epithelium (OSE). FSH may exert pleiotropic actions through its alternatively spliced receptor isoforms. Four isoforms of FSH receptors (FSHR) are reported in literature of which FSH-R1 and FSH-R3 have biological activity. Present study was undertaken to identify FSHR isoforms mediating FSH action on ovarian stem cells, using sheep OSE cells culture as the study model.MethodsCultures of sheep OSE cells (a mix of epithelial cells, VSELs, OGSCs and few contaminating red blood cells) were established with and without FSH 5IU/ml treatment. Effect of FSH treatment on self-renewal of VSELs and their differentiation into OGSCs was studied after 15 hrs by qRT-PCR using markers specific for VSELs (Oct-4A, Sox-2) and OGSCs (Oct-4). FSH receptors and its specific transcripts (R1 and R3) were studied after 3 and 15 hrs of FSH treatment by immunolocalization, in situ hybridization and qRT-PCR. FSHR and OCT-4 were also immuno-localized on sheep ovarian sections, in vitro matured follicles and early embryos.ResultsFSH treatment resulted in increased stem cells self-renewal and clonal expansion evident by the appearance of stem cell clusters. FSH receptors were expressed on ovarian stem cells whereas the epithelial cells were distinctly negative. An increase in R3 mRNA transcripts was noted after 3 hrs of FSH treatment and was reduced to basal levels by 15 hrs, whereas R1 transcript expression remained unaffected. Both FSHR and OCT-4 were immuno-localized in nuclei of stem cells, showed nuclear or ooplasmic localization in oocytes of primordial follicles and in cytoplasm of granulosa cells in growing follicles.ConclusionsFSH modulates ovarian stem cells via FSH-R3 to undergo potential self-renewal, clonal expansion as ‘cysts’ and differentiation into oocytes. OCT-4 and FSHR proteins (required initially to maintain pluripotent state of VSELs and for FSH action respectively) gradually shift from nuclei to cytoplasm of developing oocytes and are later possibly removed by surrounding granulosa cells as the oocyte prepares itself for fertilization.

show abstract

Section: Discussionmentioning

confidence: 99%