Expression of Floral Specific Genes in Tomato Hypocotyls in Liquid Culture

Pitto, Letizia; Giorgetti, Lucia; Miarelli, C.; Luccarini, G.; Colella, C.; Ronchi, Vittoria Nuti

doi:10.1007/978-94-011-0307-7_51

Cited by 1 publication

(2 citation statements)

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“…A tangible DNA loss was shown by microdensitomeric measurements of DNA content per nucleus along the different culture timepoints; a partial recovery was evident at the torpedo and plantlet stages (Nuti Ronchi et al 1992a, 1992b, Giorgetti et al 1995, Nuti Ronchi 1995. So far, all these findings, also ascertained in several other plant species (Helianthus, Prunus, Malus;Pitto et al 1994;Nuti Ronchi et al 1992c, 1992dBlando et al 1991), suggest the hypothesis that the in vitro condition induces, as a primary stress effect, the activation of a reproductive meristem, leading to the expression of floral and reproductive genes.…”

Section: Introductionmentioning

confidence: 74%

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Genome plasticity during the acquisition of embryogenic competence

Geri¹,

Turrini²,

Giorgetti³

et al. 1999

Genome

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Hypocotyl explants from carrot and other species experience concomitant segregation events and differentiation of homeotic structures during the first 20 days of culture on 2,4-dichlorophenoxyacetic acid (2,4-D). In addition to these cyto-morphological changes, significant amounts of nuclear DNA are lost, the molecular details of which we investigate in this paper. We have developed a slot-blot analysis assay to study the DNA content of a series of carrot samples; besides the leaves, this survey ranged over different culture timepoints: hypocotyls, cell lines, and somatic embryo stages. We carried on to study the relationship between this DNA loss and sequence complexity modulation. Results from probing sequences that correspond to different degrees of complexity, such as medium repetitive and unique sequences as well as sequences belonging to both classes (ribosomal cistrons, ubiquitin, actin, and chalcone synthase), consistently manifested a reduction in DNA levels during the acquisition of embryogenic competence. In some cases, the cultured cells would contain only 10% of the gene copies observed in the reference tissues. Modulation trends also showed that DNA levels of most sequences recover at the torpedo-plantlet stage, which again correlates DNA modulation and the acquisition of embryogenic competence. These results suggest that similar DNA variations may occur in plants in vivo during meiosis, possibly so that meiotic division may be properly completed.

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Section: Introductionmentioning

confidence: 74%

“…In situ hybridization with specific floral probes (Pitto et al 1994) buttress these implications. It has been seen that the tissue culture techniques induce the expression of genes belonging to floral and reproductive phases, including meiosis (Nuti Ronchi 1995).…”

Section: Discussionmentioning

confidence: 99%