Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress

Singh, Rajiv Kumar; Singh, Vivek Kumar; Sanagala, Raghavendrarao; Phanindra, Mullapudi Lakshmi Venkata; Raman, K. Venkat; Solanke, Amolkumar U.; Kumar, Polumetla Ananda; Sharma, Tilak Raj

doi:10.1007/s12010-015-1738-4

Cited by 28 publications

(7 citation statements)

References 11 publications

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“…The transgenic plants showed better growth under drought and salinity stress compared to wild-type. In order to understand the mechanisms that enable the plants to cope with drought stress, Singh R. K. et al (2015) established the role of drought-tolerant finger millet EcDehydrin7 protein in transgenic tobacco. They also observed relatively high expression of EcDehydrin7 protein compared to wild type in biochemical assays as well as qRT PCR analyses of transgenic tobacco plants, which clearly envisaged that finger millet EcDehydrin7 protein can be used in other food crops for development of drought-tolerant transgenic.…”

Section: Transgenic Developmentmentioning

confidence: 99%

Gene Discovery and Advances in Finger Millet [Eleusine coracana (L.) Gaertn.] Genomics—An Important Nutri-Cereal of Future

et al. 2016

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The rapid strides in molecular marker technologies followed by genomics, and next generation sequencing advancements in three major crops (rice, maize and wheat) of the world have given opportunities for their use in the orphan, but highly valuable future crops, including finger millet [Eleusine coracana (L.) Gaertn.]. Finger millet has many special agronomic and nutritional characteristics, which make it an indispensable crop in arid, semi-arid, hilly and tribal areas of India and Africa. The crop has proven its adaptability in harsh conditions and has shown resilience to climate change. The adaptability traits of finger millet have shown the advantage over major cereal grains under stress conditions, revealing it as a storehouse of important genomic resources for crop improvement. Although new technologies for genomic studies are now available, progress in identifying and tapping these important alleles or genes is lacking. RAPDs were the default choice for genetic diversity studies in the crop until the last decade, but the subsequent development of SSRs and comparative genomics paved the way for the marker assisted selection in finger millet. Resistance gene homologs from NBS-LRR region of finger millet for blast and sequence variants for nutritional traits from other cereals have been developed and used invariably. Population structure analysis studies exhibit 2–4 sub-populations in the finger millet gene pool with separate grouping of Indian and exotic genotypes. Recently, the omics technologies have been efficiently applied to understand the nutritional variation, drought tolerance and gene mining. Progress has also occurred with respect to transgenics development. This review presents the current biotechnological advancements along with research gaps and future perspective of genomic research in finger millet.

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Section: Transgenic Developmentmentioning

confidence: 99%

Gene Discovery and Advances in Finger Millet [Eleusine coracana (L.) Gaertn.] Genomics—An Important Nutri-Cereal of Future

et al. 2016

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“…A single gene known as -carbonic anhydrase (PgCA) was continuously upregulated in pearl millet exposed to several abiotic stimuli such as drought, salinity, and heat [57]. There are other genes which are known to be involved in drought response or tolerance in millets were EcDehydrin 7 [49], Ec-apx1 [50], EcbHLH57 [51], EcbZIP60 [52], EcGBF3 [53], EcbZIP17 [54], mt1D [55], Metallothionein, Farnesylated protein ATFP6, Farnesyl pyrophosphate synthase and Protein phosphatase 2A, RISBZ4 [56] from finger millet, SiARDP [41], SiCDPK24 [42], SiLTP [43], SiATG8a [44], SiNF-YA1 and SiNFYB8 [45], SiASR1 [46], SiASR4 [47] and SiMYB56…”

Section: Molecular Response Against Water Stress In Milletsmentioning

confidence: 99%

Drought Stress in Millets and Its Response Mechanism

Tiwari¹,

Kesarwani²,

Sharma³

et al. 2022

Advances in Plant Defense Mechanisms

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Drought is a major abiotic stress that diminishes crop yield and weakens global food security, especially in the current emerging situation of climate change as well as increases in the prevalence and severity of stress elements. Millets are nutrient-dense and capable to resist variety of harsh environmental conditions, including lack of moisture. Millet’s crop has evolved dynamically in terms of morphology, physiology, and biochemically that allow them to flee and/or adapt to adverse environmental situations. Drought stress has a significant impact on the vegetative and reproductive phases of plants. Millets generate a very low yield compared to main cereals like wheat and rice, despite their agronomic, nutritional, and health-related benefits. It is necessary to understand how these complex features are regulated and ameliorated the impact of droughts on millet productivity. Keeping this in view, the present work aims to understand the processes used for reducing the negative impacts of droughts in the production of millets varieties using advanced agronomic management strategies (use of information technology) and the biotechnology (improvements in crop genetics).

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“…Drought stress is one of the most important abiotic factors affecting plant growth and productivity. Singh R.K. et al (2015) made an effort to characterize the drought-responsive gene EcDehydrin7 of finger millet by isolating and overexpressing it in tobacco. Tobacco plants overexpressing EcDehydrin7 conferred tolerance to drought.…”

Section: Functional Characterization Of Key Genesmentioning

confidence: 99%

Finger Millet [Eleusine coracana (L.) Gaertn.] Improvement: Current Status and Future Interventions of Whole Genome Sequence

Ceasar

Maharajan²,

Krishna³

et al. 2018

Front. Plant Sci.

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The whole genome sequence (WGS) of the much awaited, nutrient rich and climate resilient crop, finger millet (Eleusine coracana (L.) Gaertn.) has been released recently. While possessing superior mineral nutrients and excellent shelf life as compared to other major cereals, multiploidy nature of the genome and relatively small plantation acreage in less developed countries hampered the genome sequencing of finger millet, disposing it as one of the lastly sequenced genomes in cereals. The genomic information available for this crop is very little when compared to other major cereals like rice, maize and barley. As a result, only a limited number of genetic and genomic studies has been undertaken for the improvement of this crop. Finger millet is known especially for its superior calcium content, but the high-throughput studies are yet to be performed to understand the mechanisms behind calcium transport and grain filling. The WGS of finger millet is expected to help to understand this and other important molecular mechanisms in finger millet, which may be harnessed for the nutrient fortification of other cereals. In this review, we discuss various efforts made so far on the improvement of finger millet including genetic improvement, transcriptome analysis, mapping of quantitative trait loci (QTLs) for traits, etc. We also discuss the pitfalls of modern genetic studies and provide insights for accelerating the finger millet improvement with the interventions of WGS in near future. Advanced genetic and genomic studies aided by WGS may help to improve the finger millet, which will be helpful to strengthen the nutritional security in addition to food security in the developing countries of Asia and Africa.

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Expression of Finger Millet EcDehydrin7 in Transgenic Tobacco Confers Tolerance to Drought Stress

Cited by 28 publications

References 11 publications

Gene Discovery and Advances in Finger Millet [Eleusine coracana (L.) Gaertn.] Genomics—An Important Nutri-Cereal of Future

Gene Discovery and Advances in Finger Millet [Eleusine coracana (L.) Gaertn.] Genomics—An Important Nutri-Cereal of Future

Drought Stress in Millets and Its Response Mechanism

Finger Millet [Eleusine coracana (L.) Gaertn.] Improvement: Current Status and Future Interventions of Whole Genome Sequence

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