Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle

Ortiz-Rey, J.A.; Suárez‐Peñaranda, José Manuel; Barús, José Ignacio Muñoz; Álvarez, Carlos; Miguel, P. San; Rodriguez‐Calvo, María Sol; Concheiro-Carro, L.

doi:10.1007/s00414-003-0403-6

Cited by 21 publications

(24 citation statements)

References 24 publications

(33 reference statements)

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“…Immunohistochemical studies on macrophage phenotypes (Betz et al 1995), fibronectin and tenascin (Ortiz-Rey et al 2003), and cytochemical analysis of mast cells (Bonelli et al 2003) have been performed. The accurate interpretation of bruises is essential at autopsy, and it is also considered that aging of bruises is an important component of many child abuse assessments.…”

Section: Introductionmentioning

confidence: 78%

Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation

et al. 2004

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We investigated mRNA expression of tissue-type plasminogen activator (tPA) and inflammatory cell dynamics for wound age estimation of bruises in mice. Neutrophils were detected from 1 h post-injury. Up to 8 h, they accumulated in subcutaneous tissue and the lower part of the dermis, and thereafter they extended to all the layers. Macrophages became detectable 3 h post-injury, and moderate infiltration of lymphocytes was seen from 144 h. In addition, epidermal thickening was also seen from 72 h. tPA mRNA expression peaked at 1 h, and increased slightly at 72 h post-injury. tPA mRNA was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, from 3 h in neutrophils and from 72 h in macrophages, respectively. This study presents the time-dependent expression of tPA mRNA in bruises in relation to temporal histologic characteristics during wound healing, which was considered to be useful for wound age estimation. Furthermore, it is suggested that tPA plays an important role in the first step of tissue remodeling.

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Section: Introductionmentioning

confidence: 78%

Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation

et al. 2004

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“…By histochemistry, the expression of vascular endothelial growth factor was found to be significantly increased above baseline only after more than 12 h upon wounding in humans [21], so it seems to be of little use for shorter survival times. The expression of fibronectin and tenascin in the dermis may be of help at early time points (15 min or more) in the rat [26], but only fibronectin appears to be of help for humans at similar time points, although with limitations in terms of sensitivity and pattern of staining [27,28]. Tenascin, on the contrary, may give information only for survival times of at least a couple of days [29].…”

Section: Discussionmentioning

confidence: 99%

Early increase in TNF-alpha-containing mast cells in skin lesions

et al. 2005

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We have evaluated the numbers and immunohistochemical positivity for tumor necrosis factor (TNF) alpha of the mast cells in (a) 40 skin samples collected at autopsy from subjects who had survived for a few seconds to 1 h, (b) 10 samples of post-mortem skin lesions and (c) 10 surgical biopsies of healthy skin. Sections were treated with fluoresceinated avidin, to tag mast cell granules, followed by indirect immunohistochemistry for TNF-alpha with polyclonal primary and rhodaminated secondary antibodies. We could confirm a progressive increase in mast cell numbers, which became significant 1 h after trauma. More important, the fraction of mast cells positive for TNF-alpha increased progressively in the same time period and became significantly higher than controls in specimens collected more than 15 min after trauma. Samples from post-mortem lesions had significantly fewer mast cells and fewer TNF-alpha-positive cells than any other group of samples. The results suggest that mast cells are quickly recruited to an injured site in response to trauma and upregulate their TNF-alpha content, which can play an early role in directing tissue response to injury. The forensic pathologist can take advantage from this behavior of mast cells for the evaluation of the timing of early vital lesions.

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“…Recently it has been shown that since certain reactions do not occur in postmortem wounds, while in life there is a complex and organized sequence of events linked to tissue repair and restoration of the function of injured organs, making it possible to characterize, step by step, morphological and functional stages, and enabling the creation of a model that could be applied to many problems in legal or forensic medicine (Oehmichen and Kirchner 1996;Ortiz-Rey et al, 2002, 2003Oehmichen, 2004;Kondo, 2007).…”

Section: Introductionmentioning

confidence: 99%

Histological analysis of short-term vital reactions in skin wounds: potential applications in forensic work

et al. 2011

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In forensic medical work, in cases with homicide suspicion, it is important to be able to determine with the greatest possible precision when injuries occurred and whether during vital, or post-mortem conditions. Although several markers of vitality can be employed, it has been attested that components of the extra-cellular matrix, such as fibrin, are among the earliest to be evidenced. In the present study, the histological-histochemical Mallory's Trichrome staining method, previously selected was tested to determine the presence of vital reaction in Wistar rats through fibrin accumulation by testing three short reaction time intervals: 15, 30 and 60 minutes after the skin wound infliction. For all time intervals tested, including the shortest (15 minutes), the presence of fibrin at the edges of the skin wound was evidenced. The accumulation of fibrin was, nevertheless, more pronounced at 30 and 60 minutes after the wound. It could be concluded that fibrin is a good marker for vital reaction and that it can be detected very early, within a few minutes after the injury. It is proposed that histological method coupled to the histochemical staining technique here tested can be incorporated into routine forensic work as a tool for evidencing the existence or not of vital reaction.Keywords: vital reaction, skin wound, histological analysis, fibrin.Análise histológica da reação vital em ferimentos da pele em curto intervalo de tempo: aplicações potenciais em trabalho forense ResumoNo trabalho médico-forense, um aspecto importante em casos de suspeita de homicídios é a determinação, com a maior precisão possível, de quando ocorreram os ferimentos e se em condições de plena vitalidade ou se post-mortem. Embora diversos marcadores possam ser utilizados para o diagnóstico de vitalidade das lesões, alguns componentes da matriz extracelular, como a fibrina, podem ser os primeiros sinais de reação vital a serem evidenciados. No presente estudo, o método de coloração histológica-histoquímica Tricrômio de Mallory, previamente selecionado, foi testado para determinar a presença de reação vital em ratos Whistar por meio do acúmulo da fibrina, testando-se três tempos curtos de reação: 15 , 30 e 60 minutos após a realização de ferimento na pele. Para todos os tempos testados, inclusive no tempo mais curto (15 minutos) foi evidenciado o acúmulo da fibrina na região próxima à borda do ferimento. O acúmulo de fibrina foi, no entanto, mais intenso 30 e 60 minutos após a ocorrência da lesão. Pôde-se concluir que a fibrina é um bom marcador para a reação vital, podendo ser detectada muito cedo, poucos minutos após a ocorrência do ferimento. Propõe-se que a técnica histológica acoplada à técnica histoquímica da coloração pelo Tricrômio de Mallory poderia ser facilmente incorporada à rotina do trabalho médico forense, como ferramenta para evidenciar a existência ou não de reação vital.Palavras-chave: reação vital, ferimento da pele, análise histológica, fibrina.

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Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle

Cited by 21 publications

References 24 publications

Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation

Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation

Early increase in TNF-alpha-containing mast cells in skin lesions

Histological analysis of short-term vital reactions in skin wounds: potential applications in forensic work

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